Screening survey samples for the presence of barley yellow dwarf viruses
In Purdue University laboratory, enzyme-linked immunosorbent assay (ELISA) is the most successful procedure for routine surveys of the ocurrence of barley yellow dwarf virus (BYDV). Polyclonal antisera are used but the potential of monoclonal antibodies with defined reactivity is being considered. Handling and extraction procedures for samples are described. Some host variables that have to be considered in sample analysis are: different hosts of BYDV differ considerably in virus content; some resistant cereal cultivars contain much smaller amounts of virus than susceptible ones; virus content varies with age at infection and with incubation time; roots usually contain more virus than leaves; virus yields depend on sample condition and storage; virus content can vary among different leaves of the same plant. In surveys, ELISA activity survives well in leaf tissue even if it is frozen or dried, a condition that makes it feasible to transport samples for testing. However, information on the reduction of virus reactivity or cross-reactivity to BYDV antisera is needed for samples after transportation. Transfer of dry leaf tissue presents no quarantine hazard. (EN)
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México, D.F. (México)
1984
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Subjects: | DIAGNOSTICO, HORDEUM VULGARE, VIRUS ENANISMO AMARILLO DE CEBADA, VIROSIS, ELISA, AISLAMIENTO (TECNICA), TECNICAS INMUNOENZIMATICAS, ESTADOS UNIDOS DE AMERICA, |
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KOHA-OAI-BVE:431182020-02-03T21:06:35ZScreening survey samples for the presence of barley yellow dwarf virusesBarley yellow dwarf; a proceedings of the workshop 87005 Lister, R.M. 58027 Clement, D. 119628 Skaria, M. 52048 Burnett, P.A. 60314 Cuellar, E. eds. 5241 CIMMYT, México, D.F. (México) 34214 Barley Yellow Dwarf Workshop México, D.F. (México) 6-8 Dic 1983 México, D.F. (México)1984In Purdue University laboratory, enzyme-linked immunosorbent assay (ELISA) is the most successful procedure for routine surveys of the ocurrence of barley yellow dwarf virus (BYDV). Polyclonal antisera are used but the potential of monoclonal antibodies with defined reactivity is being considered. Handling and extraction procedures for samples are described. Some host variables that have to be considered in sample analysis are: different hosts of BYDV differ considerably in virus content; some resistant cereal cultivars contain much smaller amounts of virus than susceptible ones; virus content varies with age at infection and with incubation time; roots usually contain more virus than leaves; virus yields depend on sample condition and storage; virus content can vary among different leaves of the same plant. In surveys, ELISA activity survives well in leaf tissue even if it is frozen or dried, a condition that makes it feasible to transport samples for testing. However, information on the reduction of virus reactivity or cross-reactivity to BYDV antisera is needed for samples after transportation. Transfer of dry leaf tissue presents no quarantine hazard. (EN)In Purdue University laboratory, enzyme-linked immunosorbent assay (ELISA) is the most successful procedure for routine surveys of the ocurrence of barley yellow dwarf virus (BYDV). Polyclonal antisera are used but the potential of monoclonal antibodies with defined reactivity is being considered. Handling and extraction procedures for samples are described. Some host variables that have to be considered in sample analysis are: different hosts of BYDV differ considerably in virus content; some resistant cereal cultivars contain much smaller amounts of virus than susceptible ones; virus content varies with age at infection and with incubation time; roots usually contain more virus than leaves; virus yields depend on sample condition and storage; virus content can vary among different leaves of the same plant. In surveys, ELISA activity survives well in leaf tissue even if it is frozen or dried, a condition that makes it feasible to transport samples for testing. However, information on the reduction of virus reactivity or cross-reactivity to BYDV antisera is needed for samples after transportation. Transfer of dry leaf tissue presents no quarantine hazard. (EN)DIAGNOSTICOHORDEUM VULGAREVIRUS ENANISMO AMARILLO DE CEBADAVIROSISELISAAISLAMIENTO (TECNICA)TECNICAS INMUNOENZIMATICASESTADOS UNIDOS DE AMERICA |
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DIAGNOSTICO HORDEUM VULGARE VIRUS ENANISMO AMARILLO DE CEBADA VIROSIS ELISA AISLAMIENTO (TECNICA) TECNICAS INMUNOENZIMATICAS ESTADOS UNIDOS DE AMERICA DIAGNOSTICO HORDEUM VULGARE VIRUS ENANISMO AMARILLO DE CEBADA VIROSIS ELISA AISLAMIENTO (TECNICA) TECNICAS INMUNOENZIMATICAS ESTADOS UNIDOS DE AMERICA |
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DIAGNOSTICO HORDEUM VULGARE VIRUS ENANISMO AMARILLO DE CEBADA VIROSIS ELISA AISLAMIENTO (TECNICA) TECNICAS INMUNOENZIMATICAS ESTADOS UNIDOS DE AMERICA DIAGNOSTICO HORDEUM VULGARE VIRUS ENANISMO AMARILLO DE CEBADA VIROSIS ELISA AISLAMIENTO (TECNICA) TECNICAS INMUNOENZIMATICAS ESTADOS UNIDOS DE AMERICA 87005 Lister, R.M. 58027 Clement, D. 119628 Skaria, M. 52048 Burnett, P.A. 60314 Cuellar, E. eds. 5241 CIMMYT, México, D.F. (México) 34214 Barley Yellow Dwarf Workshop México, D.F. (México) 6-8 Dic 1983 Screening survey samples for the presence of barley yellow dwarf viruses |
description |
In Purdue University laboratory, enzyme-linked immunosorbent assay (ELISA) is the most successful procedure for routine surveys of the ocurrence of barley yellow dwarf virus (BYDV). Polyclonal antisera are used but the potential of monoclonal antibodies with defined reactivity is being considered. Handling and extraction procedures for samples are described. Some host variables that have to be considered in sample analysis are: different hosts of BYDV differ considerably in virus content; some resistant cereal cultivars contain much smaller amounts of virus than susceptible ones; virus content varies with age at infection and with incubation time; roots usually contain more virus than leaves; virus yields depend on sample condition and storage; virus content can vary among different leaves of the same plant. In surveys, ELISA activity survives well in leaf tissue even if it is frozen or dried, a condition that makes it feasible to transport samples for testing. However, information on the reduction of virus reactivity or cross-reactivity to BYDV antisera is needed for samples after transportation. Transfer of dry leaf tissue presents no quarantine hazard. (EN) |
format |
|
topic_facet |
DIAGNOSTICO HORDEUM VULGARE VIRUS ENANISMO AMARILLO DE CEBADA VIROSIS ELISA AISLAMIENTO (TECNICA) TECNICAS INMUNOENZIMATICAS ESTADOS UNIDOS DE AMERICA |
author |
87005 Lister, R.M. 58027 Clement, D. 119628 Skaria, M. 52048 Burnett, P.A. 60314 Cuellar, E. eds. 5241 CIMMYT, México, D.F. (México) 34214 Barley Yellow Dwarf Workshop México, D.F. (México) 6-8 Dic 1983 |
author_facet |
87005 Lister, R.M. 58027 Clement, D. 119628 Skaria, M. 52048 Burnett, P.A. 60314 Cuellar, E. eds. 5241 CIMMYT, México, D.F. (México) 34214 Barley Yellow Dwarf Workshop México, D.F. (México) 6-8 Dic 1983 |
author_sort |
87005 Lister, R.M. |
title |
Screening survey samples for the presence of barley yellow dwarf viruses |
title_short |
Screening survey samples for the presence of barley yellow dwarf viruses |
title_full |
Screening survey samples for the presence of barley yellow dwarf viruses |
title_fullStr |
Screening survey samples for the presence of barley yellow dwarf viruses |
title_full_unstemmed |
Screening survey samples for the presence of barley yellow dwarf viruses |
title_sort |
screening survey samples for the presence of barley yellow dwarf viruses |
publisher |
México, D.F. (México) |
publishDate |
1984 |
work_keys_str_mv |
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1756053670885064705 |