Serologic diagnosis of bovine tuberculosis :

The passive hemagglutination (PHA) test, hemolytic modification (HL), kaolin agglutination (KA) test, and complement-fixation (CF) test were studied for the diagnosis of bovine tuberculosis. The KA test was the most reliable in detecting tuberculous cattle, whereas PHA, HL, and CF tests did not give sharp distinction between titers from tuberculin reactors without lesions, tuberculous cattle and healthy cattle. On the other hand, in instances of KA testing, nonspecific KA was observed in some healthy cattle from tuberculosis-free districts. The nonspecific agglutinin was found in the 19S fraction of serum and could be eliminated by adsorption with thick kaolin suspension (1 mg/ml) before the KA test was done. Specificity of the 19S antibody against phosphatide from tubercle bacilli seemed better than that of the 19S polysaccharide antibody and of the 7S antibody against phosphatide and polysaccharide fractions. Serum samples from both artificially and naturally infected cattle prepared from blood collected 1 to 2 weeks after tuberculin testing contained fourfold or greater quantities of antibody than serum prepared from blood collected before tuberculin testing

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Main Authors: 132726 Yugi, H., 99857 Nozaki, C.
Format: biblioteca
Language:eng
Published: Jul
Subjects:DIAGNOSTICO DE LABORATORIO, INMUNODIAGNOSTICO, TUBERCULOSIS BOVINA, PRUEBA TUBERCULINICA, TECNICAS INMUNOLOGICAS, PRUEBA DE HEMAGLUTINACION, PRUEBA DE FIJACION DEL COMPLEMENTO, REACCIONES DE AGLUTINACION, ESPECIFICIDAD, REACCION ANTIGENO-ANTICUERPO, ANTICUERPOS, GANADO BOVINO,
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spelling KOHA-OAI-BVE:426402020-02-03T21:06:00ZSerologic diagnosis of bovine tuberculosis : 132726 Yugi, H. 99857 Nozaki, C. Jul 1972engThe passive hemagglutination (PHA) test, hemolytic modification (HL), kaolin agglutination (KA) test, and complement-fixation (CF) test were studied for the diagnosis of bovine tuberculosis. The KA test was the most reliable in detecting tuberculous cattle, whereas PHA, HL, and CF tests did not give sharp distinction between titers from tuberculin reactors without lesions, tuberculous cattle and healthy cattle. On the other hand, in instances of KA testing, nonspecific KA was observed in some healthy cattle from tuberculosis-free districts. The nonspecific agglutinin was found in the 19S fraction of serum and could be eliminated by adsorption with thick kaolin suspension (1 mg/ml) before the KA test was done. Specificity of the 19S antibody against phosphatide from tubercle bacilli seemed better than that of the 19S polysaccharide antibody and of the 7S antibody against phosphatide and polysaccharide fractions. Serum samples from both artificially and naturally infected cattle prepared from blood collected 1 to 2 weeks after tuberculin testing contained fourfold or greater quantities of antibody than serum prepared from blood collected before tuberculin testingThe passive hemagglutination (PHA) test, hemolytic modification (HL), kaolin agglutination (KA) test, and complement-fixation (CF) test were studied for the diagnosis of bovine tuberculosis. The KA test was the most reliable in detecting tuberculous cattle, whereas PHA, HL, and CF tests did not give sharp distinction between titers from tuberculin reactors without lesions, tuberculous cattle and healthy cattle. On the other hand, in instances of KA testing, nonspecific KA was observed in some healthy cattle from tuberculosis-free districts. The nonspecific agglutinin was found in the 19S fraction of serum and could be eliminated by adsorption with thick kaolin suspension (1 mg/ml) before the KA test was done. Specificity of the 19S antibody against phosphatide from tubercle bacilli seemed better than that of the 19S polysaccharide antibody and of the 7S antibody against phosphatide and polysaccharide fractions. Serum samples from both artificially and naturally infected cattle prepared from blood collected 1 to 2 weeks after tuberculin testing contained fourfold or greater quantities of antibody than serum prepared from blood collected before tuberculin testingDIAGNOSTICO DE LABORATORIOINMUNODIAGNOSTICOTUBERCULOSIS BOVINAPRUEBA TUBERCULINICATECNICAS INMUNOLOGICASPRUEBA DE HEMAGLUTINACIONPRUEBA DE FIJACION DEL COMPLEMENTOREACCIONES DE AGLUTINACIONESPECIFICIDADREACCION ANTIGENO-ANTICUERPOANTICUERPOSGANADO BOVINOAmerican Journal of Veterinary Research (EUA)
institution IICA
collection Koha
country Costa Rica
countrycode CR
component Bibliográfico
access En linea
databasecode cat-sibiica
tag biblioteca
region America Central
libraryname Sistema de Bibliotecas IICA/CATIE
language eng
topic DIAGNOSTICO DE LABORATORIO
INMUNODIAGNOSTICO
TUBERCULOSIS BOVINA
PRUEBA TUBERCULINICA
TECNICAS INMUNOLOGICAS
PRUEBA DE HEMAGLUTINACION
PRUEBA DE FIJACION DEL COMPLEMENTO
REACCIONES DE AGLUTINACION
ESPECIFICIDAD
REACCION ANTIGENO-ANTICUERPO
ANTICUERPOS
GANADO BOVINO
DIAGNOSTICO DE LABORATORIO
INMUNODIAGNOSTICO
TUBERCULOSIS BOVINA
PRUEBA TUBERCULINICA
TECNICAS INMUNOLOGICAS
PRUEBA DE HEMAGLUTINACION
PRUEBA DE FIJACION DEL COMPLEMENTO
REACCIONES DE AGLUTINACION
ESPECIFICIDAD
REACCION ANTIGENO-ANTICUERPO
ANTICUERPOS
GANADO BOVINO
spellingShingle DIAGNOSTICO DE LABORATORIO
INMUNODIAGNOSTICO
TUBERCULOSIS BOVINA
PRUEBA TUBERCULINICA
TECNICAS INMUNOLOGICAS
PRUEBA DE HEMAGLUTINACION
PRUEBA DE FIJACION DEL COMPLEMENTO
REACCIONES DE AGLUTINACION
ESPECIFICIDAD
REACCION ANTIGENO-ANTICUERPO
ANTICUERPOS
GANADO BOVINO
DIAGNOSTICO DE LABORATORIO
INMUNODIAGNOSTICO
TUBERCULOSIS BOVINA
PRUEBA TUBERCULINICA
TECNICAS INMUNOLOGICAS
PRUEBA DE HEMAGLUTINACION
PRUEBA DE FIJACION DEL COMPLEMENTO
REACCIONES DE AGLUTINACION
ESPECIFICIDAD
REACCION ANTIGENO-ANTICUERPO
ANTICUERPOS
GANADO BOVINO
132726 Yugi, H.
99857 Nozaki, C.
Serologic diagnosis of bovine tuberculosis :
description The passive hemagglutination (PHA) test, hemolytic modification (HL), kaolin agglutination (KA) test, and complement-fixation (CF) test were studied for the diagnosis of bovine tuberculosis. The KA test was the most reliable in detecting tuberculous cattle, whereas PHA, HL, and CF tests did not give sharp distinction between titers from tuberculin reactors without lesions, tuberculous cattle and healthy cattle. On the other hand, in instances of KA testing, nonspecific KA was observed in some healthy cattle from tuberculosis-free districts. The nonspecific agglutinin was found in the 19S fraction of serum and could be eliminated by adsorption with thick kaolin suspension (1 mg/ml) before the KA test was done. Specificity of the 19S antibody against phosphatide from tubercle bacilli seemed better than that of the 19S polysaccharide antibody and of the 7S antibody against phosphatide and polysaccharide fractions. Serum samples from both artificially and naturally infected cattle prepared from blood collected 1 to 2 weeks after tuberculin testing contained fourfold or greater quantities of antibody than serum prepared from blood collected before tuberculin testing
format
topic_facet DIAGNOSTICO DE LABORATORIO
INMUNODIAGNOSTICO
TUBERCULOSIS BOVINA
PRUEBA TUBERCULINICA
TECNICAS INMUNOLOGICAS
PRUEBA DE HEMAGLUTINACION
PRUEBA DE FIJACION DEL COMPLEMENTO
REACCIONES DE AGLUTINACION
ESPECIFICIDAD
REACCION ANTIGENO-ANTICUERPO
ANTICUERPOS
GANADO BOVINO
author 132726 Yugi, H.
99857 Nozaki, C.
author_facet 132726 Yugi, H.
99857 Nozaki, C.
author_sort 132726 Yugi, H.
title Serologic diagnosis of bovine tuberculosis :
title_short Serologic diagnosis of bovine tuberculosis :
title_full Serologic diagnosis of bovine tuberculosis :
title_fullStr Serologic diagnosis of bovine tuberculosis :
title_full_unstemmed Serologic diagnosis of bovine tuberculosis :
title_sort serologic diagnosis of bovine tuberculosis :
publishDate Jul
work_keys_str_mv AT 132726yugih serologicdiagnosisofbovinetuberculosis
AT 99857nozakic serologicdiagnosisofbovinetuberculosis
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