RFLP marker-assisted breeding for maize virus resistance
The use of restriction fragment length polymorphisms (RFLPs) has been proposed as a means of enhancing the success of plant breeding. This paper discusses the rationale involved in deciding whether or not to use RFLPs to assist the improvement of yield and of host plant resistance to two maize viral pathogens. A present, a cost-effective argument probably cannot be made for the use of RFLPs to assist selection for yield. For maize dwarf mosaic virus (MDMV), a tightly bracketed major gene for resistance has been mapped to the short arm of chromosome 6. However, there is not a compelling argument to use RFLPs to assist in the selection for this trait because artificial inoculation is effective, resistance is largely dominant, and modifiers apparently control the expression of resistance in different susceptible genetic backgrounds. No resistance factors have been described for resistance to maize chlorotic dwarf virus (MCDV). Because of the effort and expense involved in screening for MCDV resistance, it may be more cost effective to establish and use RFLP markers tightly linked to loci conferring MCDV resistance in breeding MCDV-resistant lines
| Main Authors: | , , , , , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Published: |
Ibadan (Nigeria) IITA
1992
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| Summary: | The use of restriction fragment length polymorphisms (RFLPs) has been proposed as a means of enhancing the success of plant breeding. This paper discusses the rationale involved in deciding whether or not to use RFLPs to assist the improvement of yield and of host plant resistance to two maize viral pathogens. A present, a cost-effective argument probably cannot be made for the use of RFLPs to assist selection for yield. For maize dwarf mosaic virus (MDMV), a tightly bracketed major gene for resistance has been mapped to the short arm of chromosome 6. However, there is not a compelling argument to use RFLPs to assist in the selection for this trait because artificial inoculation is effective, resistance is largely dominant, and modifiers apparently control the expression of resistance in different susceptible genetic backgrounds. No resistance factors have been described for resistance to maize chlorotic dwarf virus (MCDV). Because of the effort and expense involved in screening for MCDV resistance, it may be more cost effective to establish and use RFLP markers tightly linked to loci conferring MCDV resistance in breeding MCDV-resistant lines |
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