Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana
Long-distance signaling via phloem tissues is an important mechanism for inter-organ communication. Such communication allows plants to integrate environmental information into physiological and developmental responses. Grafting has provided persuasive evidence of long-distance signaling involved in various processes, including flowering, tuberization, nodulation, shoot branching, post-transcriptional gene silencing, and disease resistance. A micro-grafting technique to generate two-shoot grafts is available for young seedlings of Arabidopsis thaliana and was adapted for use in the study of flowering. Histological analysis using transgenic plants expressing b-glucuronidase (GUS) in phloem tissues showed that phloem continuity between a stock and a scion was established between 7 and 10 days after grafting. Experiments using tracer dyes and enhanced green fluorescent protein (EGFP) showed that the phloem connection was functional and capable of effecting macromolecular transmission. Successful grafts can be obtained at high frequency (10–30%) and selected after 2–3 weeks of post-surgery growth. This method was applied successfully to the study of flowering, one of the important events regulated by long-distance signaling. This grafting technique will facilitate the study of the long-distance action of genes involved in various aspects of growth and development, and in transport of signal molecules.
Main Authors: | , , , |
---|---|
Format: | biblioteca |
Language: | | 0 |
Published: |
Japón: The Botanical Society of Japan, Springer,
2009
|
Subjects: | ARABIDOPSIS THALIANA, INJERTO, PLANTULAS, FLORACION, FLOEMA, PLANTAS TRANSGENICAS, RESPUESTA FISIOLOGICA, FISIOLOGIA VEGETAL, DESARROLLO BIOLOGICO, |
Online Access: | https://doi.org/10.1007/s10265-008-0209-1 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
KOHA-OAI-BVE:125414 |
---|---|
record_format |
koha |
spelling |
KOHA-OAI-BVE:1254142020-12-07T16:49:30ZAdaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana 99759 Notaguchi, M. 60997 Daimon, Y. 39614 Abe, M. 43363 Araki, T. Japón: The Botanical Society of Japan, Springer, 2009| 0pdfLong-distance signaling via phloem tissues is an important mechanism for inter-organ communication. Such communication allows plants to integrate environmental information into physiological and developmental responses. Grafting has provided persuasive evidence of long-distance signaling involved in various processes, including flowering, tuberization, nodulation, shoot branching, post-transcriptional gene silencing, and disease resistance. A micro-grafting technique to generate two-shoot grafts is available for young seedlings of Arabidopsis thaliana and was adapted for use in the study of flowering. Histological analysis using transgenic plants expressing b-glucuronidase (GUS) in phloem tissues showed that phloem continuity between a stock and a scion was established between 7 and 10 days after grafting. Experiments using tracer dyes and enhanced green fluorescent protein (EGFP) showed that the phloem connection was functional and capable of effecting macromolecular transmission. Successful grafts can be obtained at high frequency (10–30%) and selected after 2–3 weeks of post-surgery growth. This method was applied successfully to the study of flowering, one of the important events regulated by long-distance signaling. This grafting technique will facilitate the study of the long-distance action of genes involved in various aspects of growth and development, and in transport of signal molecules. Incluye 50 referencias bibliográficas en las páginas 213-214Long-distance signaling via phloem tissues is an important mechanism for inter-organ communication. Such communication allows plants to integrate environmental information into physiological and developmental responses. Grafting has provided persuasive evidence of long-distance signaling involved in various processes, including flowering, tuberization, nodulation, shoot branching, post-transcriptional gene silencing, and disease resistance. A micro-grafting technique to generate two-shoot grafts is available for young seedlings of Arabidopsis thaliana and was adapted for use in the study of flowering. Histological analysis using transgenic plants expressing b-glucuronidase (GUS) in phloem tissues showed that phloem continuity between a stock and a scion was established between 7 and 10 days after grafting. Experiments using tracer dyes and enhanced green fluorescent protein (EGFP) showed that the phloem connection was functional and capable of effecting macromolecular transmission. Successful grafts can be obtained at high frequency (10–30%) and selected after 2–3 weeks of post-surgery growth. This method was applied successfully to the study of flowering, one of the important events regulated by long-distance signaling. This grafting technique will facilitate the study of the long-distance action of genes involved in various aspects of growth and development, and in transport of signal molecules. ARABIDOPSIS THALIANAINJERTOPLANTULASFLORACIONFLOEMAPLANTAS TRANSGENICASRESPUESTA FISIOLOGICAFISIOLOGIA VEGETALDESARROLLO BIOLOGICOJournal of Plant Research (Japan)https://doi.org/10.1007/s10265-008-0209-1 |
institution |
IICA |
collection |
Koha |
country |
Costa Rica |
countrycode |
CR |
component |
Bibliográfico |
access |
En linea En linea |
databasecode |
cat-sibiica |
tag |
biblioteca |
region |
America Central |
libraryname |
Sistema de Bibliotecas IICA/CATIE |
language |
| 0 |
topic |
ARABIDOPSIS THALIANA INJERTO PLANTULAS FLORACION FLOEMA PLANTAS TRANSGENICAS RESPUESTA FISIOLOGICA FISIOLOGIA VEGETAL DESARROLLO BIOLOGICO ARABIDOPSIS THALIANA INJERTO PLANTULAS FLORACION FLOEMA PLANTAS TRANSGENICAS RESPUESTA FISIOLOGICA FISIOLOGIA VEGETAL DESARROLLO BIOLOGICO |
spellingShingle |
ARABIDOPSIS THALIANA INJERTO PLANTULAS FLORACION FLOEMA PLANTAS TRANSGENICAS RESPUESTA FISIOLOGICA FISIOLOGIA VEGETAL DESARROLLO BIOLOGICO ARABIDOPSIS THALIANA INJERTO PLANTULAS FLORACION FLOEMA PLANTAS TRANSGENICAS RESPUESTA FISIOLOGICA FISIOLOGIA VEGETAL DESARROLLO BIOLOGICO 99759 Notaguchi, M. 60997 Daimon, Y. 39614 Abe, M. 43363 Araki, T. Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana |
description |
Long-distance signaling via phloem tissues is an important mechanism for inter-organ communication. Such communication allows plants to integrate environmental information into physiological and developmental responses. Grafting has provided persuasive evidence of long-distance signaling involved in various processes, including flowering, tuberization, nodulation, shoot branching, post-transcriptional gene silencing, and disease resistance. A micro-grafting technique to generate two-shoot grafts is available for young seedlings of Arabidopsis thaliana and was adapted for use in the study of flowering. Histological analysis using transgenic plants expressing b-glucuronidase (GUS) in phloem tissues showed that phloem continuity between a stock and a scion was
established between 7 and 10 days after grafting. Experiments using tracer dyes and enhanced green fluorescent protein (EGFP) showed that the phloem connection was functional and capable of effecting macromolecular transmission. Successful grafts can be obtained at high frequency (10–30%) and selected after 2–3 weeks of post-surgery growth. This method was applied successfully to the study of flowering, one of the important events regulated by long-distance signaling. This grafting technique will facilitate the study of the long-distance action of genes involved in various aspects of growth and development, and in transport of signal molecules.
|
format |
|
topic_facet |
ARABIDOPSIS THALIANA INJERTO PLANTULAS FLORACION FLOEMA PLANTAS TRANSGENICAS RESPUESTA FISIOLOGICA FISIOLOGIA VEGETAL DESARROLLO BIOLOGICO |
author |
99759 Notaguchi, M. 60997 Daimon, Y. 39614 Abe, M. 43363 Araki, T. |
author_facet |
99759 Notaguchi, M. 60997 Daimon, Y. 39614 Abe, M. 43363 Araki, T. |
author_sort |
99759 Notaguchi, M. |
title |
Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana |
title_short |
Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana |
title_full |
Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana |
title_fullStr |
Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana |
title_full_unstemmed |
Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana |
title_sort |
adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of arabidopsis thaliana |
publisher |
Japón: The Botanical Society of Japan, Springer, |
publishDate |
2009 |
url |
https://doi.org/10.1007/s10265-008-0209-1 |
work_keys_str_mv |
AT 99759notaguchim adaptationofaseedlingmicrograftingtechniquetothestudyoflongdistancesignalinginfloweringofarabidopsisthaliana AT 60997daimony adaptationofaseedlingmicrograftingtechniquetothestudyoflongdistancesignalinginfloweringofarabidopsisthaliana AT 39614abem adaptationofaseedlingmicrograftingtechniquetothestudyoflongdistancesignalinginfloweringofarabidopsisthaliana AT 43363arakit adaptationofaseedlingmicrograftingtechniquetothestudyoflongdistancesignalinginfloweringofarabidopsisthaliana |
_version_ |
1756064788962607105 |