Molecular and structural characterization of expansins modulated by fungal endophytes in the Antarctic Colobanthus quitensis [Kunth] Bartl. Exposed to drought stress

Molecular and structural characterization of expansins modulated by fungal endophytes in the Antarctic Colobanthus quitensis [Kunth] Bartl. Exposed to drought stress

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Expansins are proteins involved in cell wall metabolism that play an important role in plant growth, development, fruit ripening and abiotic stress tolerance. In the present study, we analyzed putative expansins that respond to drought stress. Five expansin genes were identified in cDNA libraries isolated from Colobanthus quitensis gown either with or without endophytic fungi under hydric stress. A differential transcript abundance was observed by qPCR analysis upon drought stress. To compare these expansin genes, and to suggest a possible mechanism of action at the molecular level, the structural model of the deduced proteins was obtained by comparative modeling methodology. The structures showed two domains and an open groove on the surface of the proteins was observed in the five structural models. The proteins were evaluated in terms of their proteinligand interactions using four different ligands. The results suggested differences in their mode of proteinligand interaction, in particular concerning the residues involved in the protein-ligand interaction. The presented evidence supports the participation of some members of the expansin multiprotein family in the response to drought stress in C. quitensis and suggest that the response is modulated by endophytic fungi.

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Main Authors: Morales Quintana, Luis, Barrera, Andrea, Hereme, Rasme, Jara, Karla, Rivera Mora, Claudia Marisol, Valenzuela Riffo, Felipe, Gundel, Pedro Emilio, Pollmann, Stephan, Ramos, Patricio
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Subjects:CELL WALL, CLIMATE CHANGE, DROUGHT STRESS, FUNGAL ENDOPHYTES, MOLECULAR RESPONSE, ,
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spelling KOHA-OAI-AGRO:554972023-10-06T10:17:10Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=55497http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGMolecular and structural characterization of expansins modulated by fungal endophytes in the Antarctic Colobanthus quitensis [Kunth] Bartl. Exposed to drought stressMorales Quintana, LuisBarrera, AndreaHereme, RasmeJara, KarlaRivera Mora, Claudia MarisolValenzuela Riffo, FelipeGundel, Pedro EmilioPollmann, StephanRamos, Patriciotextengapplication/pdfapplication/pdfExpansins are proteins involved in cell wall metabolism that play an important role in plant growth, development, fruit ripening and abiotic stress tolerance. In the present study, we analyzed putative expansins that respond to drought stress. Five expansin genes were identified in cDNA libraries isolated from Colobanthus quitensis gown either with or without endophytic fungi under hydric stress. A differential transcript abundance was observed by qPCR analysis upon drought stress. To compare these expansin genes, and to suggest a possible mechanism of action at the molecular level, the structural model of the deduced proteins was obtained by comparative modeling methodology. The structures showed two domains and an open groove on the surface of the proteins was observed in the five structural models. The proteins were evaluated in terms of their proteinligand interactions using four different ligands. The results suggested differences in their mode of proteinligand interaction, in particular concerning the residues involved in the protein-ligand interaction. The presented evidence supports the participation of some members of the expansin multiprotein family in the response to drought stress in C. quitensis and suggest that the response is modulated by endophytic fungi.Expansins are proteins involved in cell wall metabolism that play an important role in plant growth, development, fruit ripening and abiotic stress tolerance. In the present study, we analyzed putative expansins that respond to drought stress. Five expansin genes were identified in cDNA libraries isolated from Colobanthus quitensis gown either with or without endophytic fungi under hydric stress. A differential transcript abundance was observed by qPCR analysis upon drought stress. To compare these expansin genes, and to suggest a possible mechanism of action at the molecular level, the structural model of the deduced proteins was obtained by comparative modeling methodology. The structures showed two domains and an open groove on the surface of the proteins was observed in the five structural models. The proteins were evaluated in terms of their proteinligand interactions using four different ligands. The results suggested differences in their mode of proteinligand interaction, in particular concerning the residues involved in the protein-ligand interaction. The presented evidence supports the participation of some members of the expansin multiprotein family in the response to drought stress in C. quitensis and suggest that the response is modulated by endophytic fungi.CELL WALLCLIMATE CHANGEDROUGHT STRESSFUNGAL ENDOPHYTESMOLECULAR RESPONSEPlant physiology and biochemistry
institution UBA FA
collection Koha
country Argentina
countrycode AR
component Bibliográfico
access En linea
En linea
databasecode cat-ceiba
tag biblioteca
region America del Sur
libraryname Biblioteca Central FAUBA
language eng
topic CELL WALL
CLIMATE CHANGE
DROUGHT STRESS
FUNGAL ENDOPHYTES
MOLECULAR RESPONSE

CELL WALL
CLIMATE CHANGE
DROUGHT STRESS
FUNGAL ENDOPHYTES
MOLECULAR RESPONSE
spellingShingle CELL WALL
CLIMATE CHANGE
DROUGHT STRESS
FUNGAL ENDOPHYTES
MOLECULAR RESPONSE

CELL WALL
CLIMATE CHANGE
DROUGHT STRESS
FUNGAL ENDOPHYTES
MOLECULAR RESPONSE
Morales Quintana, Luis
Barrera, Andrea
Hereme, Rasme
Jara, Karla
Rivera Mora, Claudia Marisol
Valenzuela Riffo, Felipe
Gundel, Pedro Emilio
Pollmann, Stephan
Ramos, Patricio
Molecular and structural characterization of expansins modulated by fungal endophytes in the Antarctic Colobanthus quitensis [Kunth] Bartl. Exposed to drought stress
description Expansins are proteins involved in cell wall metabolism that play an important role in plant growth, development, fruit ripening and abiotic stress tolerance. In the present study, we analyzed putative expansins that respond to drought stress. Five expansin genes were identified in cDNA libraries isolated from Colobanthus quitensis gown either with or without endophytic fungi under hydric stress. A differential transcript abundance was observed by qPCR analysis upon drought stress. To compare these expansin genes, and to suggest a possible mechanism of action at the molecular level, the structural model of the deduced proteins was obtained by comparative modeling methodology. The structures showed two domains and an open groove on the surface of the proteins was observed in the five structural models. The proteins were evaluated in terms of their proteinligand interactions using four different ligands. The results suggested differences in their mode of proteinligand interaction, in particular concerning the residues involved in the protein-ligand interaction. The presented evidence supports the participation of some members of the expansin multiprotein family in the response to drought stress in C. quitensis and suggest that the response is modulated by endophytic fungi.
format Texto
topic_facet
CELL WALL
CLIMATE CHANGE
DROUGHT STRESS
FUNGAL ENDOPHYTES
MOLECULAR RESPONSE
author Morales Quintana, Luis
Barrera, Andrea
Hereme, Rasme
Jara, Karla
Rivera Mora, Claudia Marisol
Valenzuela Riffo, Felipe
Gundel, Pedro Emilio
Pollmann, Stephan
Ramos, Patricio
author_facet Morales Quintana, Luis
Barrera, Andrea
Hereme, Rasme
Jara, Karla
Rivera Mora, Claudia Marisol
Valenzuela Riffo, Felipe
Gundel, Pedro Emilio
Pollmann, Stephan
Ramos, Patricio
author_sort Morales Quintana, Luis
title Molecular and structural characterization of expansins modulated by fungal endophytes in the Antarctic Colobanthus quitensis [Kunth] Bartl. Exposed to drought stress
title_short Molecular and structural characterization of expansins modulated by fungal endophytes in the Antarctic Colobanthus quitensis [Kunth] Bartl. Exposed to drought stress
title_full Molecular and structural characterization of expansins modulated by fungal endophytes in the Antarctic Colobanthus quitensis [Kunth] Bartl. Exposed to drought stress
title_fullStr Molecular and structural characterization of expansins modulated by fungal endophytes in the Antarctic Colobanthus quitensis [Kunth] Bartl. Exposed to drought stress
title_full_unstemmed Molecular and structural characterization of expansins modulated by fungal endophytes in the Antarctic Colobanthus quitensis [Kunth] Bartl. Exposed to drought stress
title_sort molecular and structural characterization of expansins modulated by fungal endophytes in the antarctic colobanthus quitensis [kunth] bartl. exposed to drought stress
url http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=55497
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
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http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
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