Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4
Soybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic stress tolerance is commercially available. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield, and was evaluated in the greenhouse and in 27 field trials in different environments in Argentina. In greenhouse experiments, transgenic plants showed increased seed yield under stress conditions together with greater epicotyl diameter, larger xylem area, and increased water use efficiency compared with controls. They also exhibited enhanced seed yield in warm and dry field conditions. This response was accompanied by an increase in seed number that was not compensated by a decrease in individual seed weight. Transcriptome analysis of plants from a field trial with maximum difference in seed yield between genotypes indicated the induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have a reduced seed yield penalty when cultivated in warm and dry conditions, which constitute the best target environments for this technology.
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KOHA-OAI-AGRO:539272022-08-10T12:56:50Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=53927http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGSuccessful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4Ribichich, Karina FabianaChiozza, MarianaÁvalos Britez, SelvaCabello, Julieta V.Arce, Augustin L.Watson, GerónimoArias, ClaudiaPortapila, MargaritaTrucco, FedericoOtegui, María ElenaChan, Raquel Líatextengapplication/pdfSoybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic stress tolerance is commercially available. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield, and was evaluated in the greenhouse and in 27 field trials in different environments in Argentina. In greenhouse experiments, transgenic plants showed increased seed yield under stress conditions together with greater epicotyl diameter, larger xylem area, and increased water use efficiency compared with controls. They also exhibited enhanced seed yield in warm and dry field conditions. This response was accompanied by an increase in seed number that was not compensated by a decrease in individual seed weight. Transcriptome analysis of plants from a field trial with maximum difference in seed yield between genotypes indicated the induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have a reduced seed yield penalty when cultivated in warm and dry conditions, which constitute the best target environments for this technology.Soybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic stress tolerance is commercially available. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield, and was evaluated in the greenhouse and in 27 field trials in different environments in Argentina. In greenhouse experiments, transgenic plants showed increased seed yield under stress conditions together with greater epicotyl diameter, larger xylem area, and increased water use efficiency compared with controls. They also exhibited enhanced seed yield in warm and dry field conditions. This response was accompanied by an increase in seed number that was not compensated by a decrease in individual seed weight. Transcriptome analysis of plants from a field trial with maximum difference in seed yield between genotypes indicated the induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have a reduced seed yield penalty when cultivated in warm and dry conditions, which constitute the best target environments for this technology.DROUGHT TOLERANCEHAHB4PHOTOSYNTHESIS RATESEED YIELD DETERMINATIONSOYBEAN FIELD TRIALSSUNFLOWER TRANSCRIPTION FACTORTRANSGENIC SOYBEANWATER USE EFFICIENCYJournal of experimental botany |
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UBA FA |
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Koha |
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Argentina |
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AR |
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Bibliográfico |
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En linea En linea |
| databasecode |
cat-ceiba |
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biblioteca |
| region |
America del Sur |
| libraryname |
Biblioteca Central FAUBA |
| language |
eng |
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DROUGHT TOLERANCE HAHB4 PHOTOSYNTHESIS RATE SEED YIELD DETERMINATION SOYBEAN FIELD TRIALS SUNFLOWER TRANSCRIPTION FACTOR TRANSGENIC SOYBEAN WATER USE EFFICIENCY DROUGHT TOLERANCE HAHB4 PHOTOSYNTHESIS RATE SEED YIELD DETERMINATION SOYBEAN FIELD TRIALS SUNFLOWER TRANSCRIPTION FACTOR TRANSGENIC SOYBEAN WATER USE EFFICIENCY |
| spellingShingle |
DROUGHT TOLERANCE HAHB4 PHOTOSYNTHESIS RATE SEED YIELD DETERMINATION SOYBEAN FIELD TRIALS SUNFLOWER TRANSCRIPTION FACTOR TRANSGENIC SOYBEAN WATER USE EFFICIENCY DROUGHT TOLERANCE HAHB4 PHOTOSYNTHESIS RATE SEED YIELD DETERMINATION SOYBEAN FIELD TRIALS SUNFLOWER TRANSCRIPTION FACTOR TRANSGENIC SOYBEAN WATER USE EFFICIENCY Ribichich, Karina Fabiana Chiozza, Mariana Ávalos Britez, Selva Cabello, Julieta V. Arce, Augustin L. Watson, Gerónimo Arias, Claudia Portapila, Margarita Trucco, Federico Otegui, María Elena Chan, Raquel Lía Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4 |
| description |
Soybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic stress tolerance is commercially available. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield, and was evaluated in the greenhouse and in 27 field trials in different environments in Argentina. In greenhouse experiments, transgenic plants showed increased seed yield under stress conditions together with greater epicotyl diameter, larger xylem area, and increased water use efficiency compared with controls. They also exhibited enhanced seed yield in warm and dry field conditions. This response was accompanied by an increase in seed number that was not compensated by a decrease in individual seed weight. Transcriptome analysis of plants from a field trial with maximum difference in seed yield between genotypes indicated the induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have a reduced seed yield penalty when cultivated in warm and dry conditions, which constitute the best target environments for this technology. |
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Texto |
| topic_facet |
DROUGHT TOLERANCE HAHB4 PHOTOSYNTHESIS RATE SEED YIELD DETERMINATION SOYBEAN FIELD TRIALS SUNFLOWER TRANSCRIPTION FACTOR TRANSGENIC SOYBEAN WATER USE EFFICIENCY |
| author |
Ribichich, Karina Fabiana Chiozza, Mariana Ávalos Britez, Selva Cabello, Julieta V. Arce, Augustin L. Watson, Gerónimo Arias, Claudia Portapila, Margarita Trucco, Federico Otegui, María Elena Chan, Raquel Lía |
| author_facet |
Ribichich, Karina Fabiana Chiozza, Mariana Ávalos Britez, Selva Cabello, Julieta V. Arce, Augustin L. Watson, Gerónimo Arias, Claudia Portapila, Margarita Trucco, Federico Otegui, María Elena Chan, Raquel Lía |
| author_sort |
Ribichich, Karina Fabiana |
| title |
Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4 |
| title_short |
Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4 |
| title_full |
Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4 |
| title_fullStr |
Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4 |
| title_full_unstemmed |
Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4 |
| title_sort |
successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor hb4 |
| url |
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=53927 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
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