Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

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In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with diferent feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same fve dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with diferent relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifcally in genes coding for debranching- and oligosaccharide-degrading enzymes. These fndings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specifcity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50°C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.

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Main Authors: Romero Victorica, Matías, Soria, Marcelo Abel, Batista García, Ramón Alberto, Ceja Navarro, Javier A., Vikram, Surendra, Ortiz, Maximiliano, Ontañon, Ornella, Ghio, Silvina
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Subjects:METAGENÓMICA, SECUENCIACIÓN DE PRÓXIMA GENERACIÓN,
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id KOHA-OAI-AGRO:53882
record_format koha
institution UBA FA
collection Koha
country Argentina
countrycode AR
component Bibliográfico
access En linea
En linea
databasecode cat-ceiba
tag biblioteca
region America del Sur
libraryname Biblioteca Central FAUBA
language eng
topic METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
spellingShingle METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
Romero Victorica, Matías
Soria, Marcelo Abel
Batista García, Ramón Alberto
Ceja Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
description In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with diferent feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same fve dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with diferent relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifcally in genes coding for debranching- and oligosaccharide-degrading enzymes. These fndings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specifcity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50°C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.
format Texto
topic_facet METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
author Romero Victorica, Matías
Soria, Marcelo Abel
Batista García, Ramón Alberto
Ceja Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
author_facet Romero Victorica, Matías
Soria, Marcelo Abel
Batista García, Ramón Alberto
Ceja Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
author_sort Romero Victorica, Matías
title Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_short Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_full Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_fullStr Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_full_unstemmed Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_sort neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
url http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=53882
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http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
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spelling KOHA-OAI-AGRO:538822023-11-23T14:52:05Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=53882http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGNeotropical termite microbiomes as sources of novel plant cell wall degrading enzymesRomero Victorica, MatíasSoria, Marcelo AbelBatista García, Ramón AlbertoCeja Navarro, Javier A.Vikram, SurendraOrtiz, MaximilianoOntañon, OrnellaGhio, Silvinatextengapplication/pdfIn this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with diferent feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same fve dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with diferent relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifcally in genes coding for debranching- and oligosaccharide-degrading enzymes. These fndings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specifcity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50°C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with diferent feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same fve dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with diferent relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifcally in genes coding for debranching- and oligosaccharide-degrading enzymes. These fndings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specifcity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50°C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.METAGENÓMICASECUENCIACIÓN DE PRÓXIMA GENERACIÓNScientific Reports

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