Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities  application to estimate the absolute electron transport rate [ETR]

Jerez, Celia G.; García, Carolina B.; Rearte, Tomás Agustín; Figueroa, Félix L.

Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [ETR]

In order to estimate microalgal carbon assimilation or production of Chlorella fusca cultures based on electron transport rate [ETR] as in vivo chlorophyll a fluorescence, it is necessary to determine the photosynthetic yield and the absorbed quanta by measuring the incident irradiance and the fraction of absorbed light, i.e., absorptance or absorption coefficient in the photosynthetic active radiation [PAR] region of the spectra. Due to difficulties associated with the determination of light absorption, ETR is commonly expressed as relative units [rETR] although this is not a good estimator of the photosynthetic production since photobiological responses depend on the absorbed light. The quantitative filter technique [QFT] is commonly used to measure the absorbed quanta of cells retained on a filter [AbQf] as estimator of the absorbed quanta of cell suspensions [AbQs] determined by using integrating spheres. In this study, light attenuation of thin-layer cell suspensions is determined by using a measuring system designed to reduce the scattering. The light attenuation is related to the absorptance as the fraction of absorbed light by both indoor and outdoor C. fusca cultures of different cell densities. A linear relation between AbQf and AbQs [R2=0.9902, p minor to 0.01] was observed, AbQf=1.98×AbQs, being 1.98 an amplification factor to convert AbQs values into AbQf ones. On the other hand, depending on the culture system, the convenience of the use of the absorptance, light absorption or specific light absorption coefficient expressed per area thinlayer cascade or flat panel cultivators], volume [cylindrical and tubular photobioreactors], or chlorophyll units [any type of cultivation system] is discussed. The procedure for the measurement of light absorption presented in this study for C. fusca could be applied in other phytoplankton groups. The absorbed quanta as determined in this study can be used to express absolute ETR instead of relative ETR, since the first one provides much more relevant photobiological information of microalgae culture systems.

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Bibliographic Details
Main Authors:	Jerez, Celia G., García, Carolina B., Rearte, Tomás Agustín, Figueroa, Félix L.
Format:	Texto biblioteca
Language:	eng
Subjects:	ABSORPTANCE, CHLORELLA FUSCA, CHLOROPHYCEAE, ELECTRON TRANSPORT RAT, IN VIVO ABSERPTION COEFFICIENT, LIGHT ATTENUATION COEFFICIENT, ,
Online Access:	http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47340 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
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id	KOHA-OAI-AGRO:47340
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institution	UBA FA
collection	Koha
country	Argentina
countrycode	AR
component	Bibliográfico
access	En linea En linea
databasecode	cat-ceiba
tag	biblioteca
region	America del Sur
libraryname	Biblioteca Central FAUBA
language	eng
topic	ABSORPTANCE CHLORELLA FUSCA CHLOROPHYCEAE ELECTRON TRANSPORT RAT IN VIVO ABSERPTION COEFFICIENT LIGHT ATTENUATION COEFFICIENT ABSORPTANCE CHLORELLA FUSCA CHLOROPHYCEAE ELECTRON TRANSPORT RAT IN VIVO ABSERPTION COEFFICIENT LIGHT ATTENUATION COEFFICIENT
spellingShingle	ABSORPTANCE CHLORELLA FUSCA CHLOROPHYCEAE ELECTRON TRANSPORT RAT IN VIVO ABSERPTION COEFFICIENT LIGHT ATTENUATION COEFFICIENT ABSORPTANCE CHLORELLA FUSCA CHLOROPHYCEAE ELECTRON TRANSPORT RAT IN VIVO ABSERPTION COEFFICIENT LIGHT ATTENUATION COEFFICIENT Jerez, Celia G. García, Carolina B. Rearte, Tomás Agustín Figueroa, Félix L. Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [ETR]
description	In order to estimate microalgal carbon assimilation or production of Chlorella fusca cultures based on electron transport rate [ETR] as in vivo chlorophyll a fluorescence, it is necessary to determine the photosynthetic yield and the absorbed quanta by measuring the incident irradiance and the fraction of absorbed light, i.e., absorptance or absorption coefficient in the photosynthetic active radiation [PAR] region of the spectra. Due to difficulties associated with the determination of light absorption, ETR is commonly expressed as relative units [rETR] although this is not a good estimator of the photosynthetic production since photobiological responses depend on the absorbed light. The quantitative filter technique [QFT] is commonly used to measure the absorbed quanta of cells retained on a filter [AbQf] as estimator of the absorbed quanta of cell suspensions [AbQs] determined by using integrating spheres. In this study, light attenuation of thin-layer cell suspensions is determined by using a measuring system designed to reduce the scattering. The light attenuation is related to the absorptance as the fraction of absorbed light by both indoor and outdoor C. fusca cultures of different cell densities. A linear relation between AbQf and AbQs [R2=0.9902, p minor to 0.01] was observed, AbQf=1.98×AbQs, being 1.98 an amplification factor to convert AbQs values into AbQf ones. On the other hand, depending on the culture system, the convenience of the use of the absorptance, light absorption or specific light absorption coefficient expressed per area thinlayer cascade or flat panel cultivators], volume [cylindrical and tubular photobioreactors], or chlorophyll units [any type of cultivation system] is discussed. The procedure for the measurement of light absorption presented in this study for C. fusca could be applied in other phytoplankton groups. The absorbed quanta as determined in this study can be used to express absolute ETR instead of relative ETR, since the first one provides much more relevant photobiological information of microalgae culture systems.
format	Texto
topic_facet	ABSORPTANCE CHLORELLA FUSCA CHLOROPHYCEAE ELECTRON TRANSPORT RAT IN VIVO ABSERPTION COEFFICIENT LIGHT ATTENUATION COEFFICIENT
author	Jerez, Celia G. García, Carolina B. Rearte, Tomás Agustín Figueroa, Félix L.
author_facet	Jerez, Celia G. García, Carolina B. Rearte, Tomás Agustín Figueroa, Félix L.
author_sort	Jerez, Celia G.
title	Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [ETR]
title_short	Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [ETR]
title_full	Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [ETR]
title_fullStr	Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [ETR]
title_full_unstemmed	Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [ETR]
title_sort	relation between light absorption measured by the quantitative filter technique and attenuation of chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [etr]
url	http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47340 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
work_keys_str_mv	AT jerezceliag relationbetweenlightabsorptionmeasuredbythequantitativefiltertechniqueandattenuationofchlorellafuscaculturesofdifferentcelldensitiesapplicationtoestimatetheabsoluteelectrontransportrateetr AT garciacarolinab relationbetweenlightabsorptionmeasuredbythequantitativefiltertechniqueandattenuationofchlorellafuscaculturesofdifferentcelldensitiesapplicationtoestimatetheabsoluteelectrontransportrateetr AT reartetomasagustin relationbetweenlightabsorptionmeasuredbythequantitativefiltertechniqueandattenuationofchlorellafuscaculturesofdifferentcelldensitiesapplicationtoestimatetheabsoluteelectrontransportrateetr AT figueroafelixl relationbetweenlightabsorptionmeasuredbythequantitativefiltertechniqueandattenuationofchlorellafuscaculturesofdifferentcelldensitiesapplicationtoestimatetheabsoluteelectrontransportrateetr
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spelling	KOHA-OAI-AGRO:473402023-03-30T14:26:57Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47340http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGRelation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [ETR]Jerez, Celia G.García, Carolina B.Rearte, Tomás AgustínFigueroa, Félix L.textengapplication/pdfIn order to estimate microalgal carbon assimilation or production of Chlorella fusca cultures based on electron transport rate [ETR] as in vivo chlorophyll a fluorescence, it is necessary to determine the photosynthetic yield and the absorbed quanta by measuring the incident irradiance and the fraction of absorbed light, i.e., absorptance or absorption coefficient in the photosynthetic active radiation [PAR] region of the spectra. Due to difficulties associated with the determination of light absorption, ETR is commonly expressed as relative units [rETR] although this is not a good estimator of the photosynthetic production since photobiological responses depend on the absorbed light. The quantitative filter technique [QFT] is commonly used to measure the absorbed quanta of cells retained on a filter [AbQf] as estimator of the absorbed quanta of cell suspensions [AbQs] determined by using integrating spheres. In this study, light attenuation of thin-layer cell suspensions is determined by using a measuring system designed to reduce the scattering. The light attenuation is related to the absorptance as the fraction of absorbed light by both indoor and outdoor C. fusca cultures of different cell densities. A linear relation between AbQf and AbQs [R2=0.9902, p minor to 0.01] was observed, AbQf=1.98×AbQs, being 1.98 an amplification factor to convert AbQs values into AbQf ones. On the other hand, depending on the culture system, the convenience of the use of the absorptance, light absorption or specific light absorption coefficient expressed per area thinlayer cascade or flat panel cultivators], volume [cylindrical and tubular photobioreactors], or chlorophyll units [any type of cultivation system] is discussed. The procedure for the measurement of light absorption presented in this study for C. fusca could be applied in other phytoplankton groups. The absorbed quanta as determined in this study can be used to express absolute ETR instead of relative ETR, since the first one provides much more relevant photobiological information of microalgae culture systems.In order to estimate microalgal carbon assimilation or production of Chlorella fusca cultures based on electron transport rate [ETR] as in vivo chlorophyll a fluorescence, it is necessary to determine the photosynthetic yield and the absorbed quanta by measuring the incident irradiance and the fraction of absorbed light, i.e., absorptance or absorption coefficient in the photosynthetic active radiation [PAR] region of the spectra. Due to difficulties associated with the determination of light absorption, ETR is commonly expressed as relative units [rETR] although this is not a good estimator of the photosynthetic production since photobiological responses depend on the absorbed light. The quantitative filter technique [QFT] is commonly used to measure the absorbed quanta of cells retained on a filter [AbQf] as estimator of the absorbed quanta of cell suspensions [AbQs] determined by using integrating spheres. In this study, light attenuation of thin-layer cell suspensions is determined by using a measuring system designed to reduce the scattering. The light attenuation is related to the absorptance as the fraction of absorbed light by both indoor and outdoor C. fusca cultures of different cell densities. A linear relation between AbQf and AbQs [R2=0.9902, p minor to 0.01] was observed, AbQf=1.98×AbQs, being 1.98 an amplification factor to convert AbQs values into AbQf ones. On the other hand, depending on the culture system, the convenience of the use of the absorptance, light absorption or specific light absorption coefficient expressed per area thinlayer cascade or flat panel cultivators], volume [cylindrical and tubular photobioreactors], or chlorophyll units [any type of cultivation system] is discussed. The procedure for the measurement of light absorption presented in this study for C. fusca could be applied in other phytoplankton groups. The absorbed quanta as determined in this study can be used to express absolute ETR instead of relative ETR, since the first one provides much more relevant photobiological information of microalgae culture systems.ABSORPTANCECHLORELLA FUSCACHLOROPHYCEAEELECTRON TRANSPORT RATIN VIVO ABSERPTION COEFFICIENTLIGHT ATTENUATION COEFFICIENTJournal of Applied Phycology

Relation between light absorption measured by the quantitative filter technique and attenuation of Chlorella fusca cultures of different cell densities application to estimate the absolute electron transport rate [ETR]

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