Phytochrome a antagonizes PHYTOCHROME INTERACTING FACTOR 1 to prevent over - activation of photomorphogenesis
Phytochrome A [phyA] is crucial to initiate the early steps of the transition between skoto- and photomorphogenesis upon light exposure and to complete this process under far-red light [typical of dense vegetation canopies]. However, under prolonged red or white light, phyA mutants are hyper-photomorphogenic in many respects. To investigate this issue, we analyzed the late response of the transcriptome of the phyA mutant to red light. Compared to the wild-type [WT], hyper-responsive genes outnumbered the genes showing reduced response to red light in phyA. A network analysis revealed the co-expression of PHYTOCHROME INTERACTING FACTOR 1 [PIF1] with those genes showing hyper-promotion by red light in phyA. The enhanced responses of gene expression, cotyledon unfolding, hypocotyl growth, and greening observed in the phyA mutant compared to the WT were absent in the phyA pif1 double mutant compared to pif1, indicating that the hyper-photomorphogenic phenotype of phyA requires PIF1. PIF1 directly binds to gene promoters that displayed PIF1-mediated enhanced response to red light. Expression of mutant PIF1 deficient in interactions with phyA and phyB enhanced the long-term growth response to red light but reduced the expression of selected genes in response to red light. We propose that phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis during early seedling development.
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| Subjects: | SEEDLING, RADIATION RESPONSE, PROTEOLYSIS, PROTEIN TERTIARY STRUCTURE, PROTEIN STRUCTURE, TERTIARY, PROTEIN STABILITY, PROTEIN DEGRADATION, PIF1 PROTEIN, ARABIDOPSIS, PIF1, PHYTOCHROME B, PHYTOCHROME A, PHYA, MUTATION, METABOLISM, LIGHT [GROWTH], LIGHT [GENE EXPRESSION], LIGHT, GROWTH, DEVELOPMENT AND AGING, GENETICS, GENE EXPRESSION REGULATION, PLANT, GENE EXPRESSION REGULATION, DE-ETIOLATION, CHEMISTRY, BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS, ARABIDOPSIS PROTEINS, ARABIDOPSIS, , |
| Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47214 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
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KOHA-OAI-AGRO:472142022-10-26T11:23:39Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47214http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGPhytochrome a antagonizes PHYTOCHROME INTERACTING FACTOR 1 to prevent over - activation of photomorphogenesisKrzymuski, MartínCerdán, Pablo D.Zhu, L.Vinh, A.Chory, J.Huq, E.Casal, Jorge Josétextspaapplication/pdfPhytochrome A [phyA] is crucial to initiate the early steps of the transition between skoto- and photomorphogenesis upon light exposure and to complete this process under far-red light [typical of dense vegetation canopies]. However, under prolonged red or white light, phyA mutants are hyper-photomorphogenic in many respects. To investigate this issue, we analyzed the late response of the transcriptome of the phyA mutant to red light. Compared to the wild-type [WT], hyper-responsive genes outnumbered the genes showing reduced response to red light in phyA. A network analysis revealed the co-expression of PHYTOCHROME INTERACTING FACTOR 1 [PIF1] with those genes showing hyper-promotion by red light in phyA. The enhanced responses of gene expression, cotyledon unfolding, hypocotyl growth, and greening observed in the phyA mutant compared to the WT were absent in the phyA pif1 double mutant compared to pif1, indicating that the hyper-photomorphogenic phenotype of phyA requires PIF1. PIF1 directly binds to gene promoters that displayed PIF1-mediated enhanced response to red light. Expression of mutant PIF1 deficient in interactions with phyA and phyB enhanced the long-term growth response to red light but reduced the expression of selected genes in response to red light. We propose that phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis during early seedling development.Phytochrome A [phyA] is crucial to initiate the early steps of the transition between skoto- and photomorphogenesis upon light exposure and to complete this process under far-red light [typical of dense vegetation canopies]. However, under prolonged red or white light, phyA mutants are hyper-photomorphogenic in many respects. To investigate this issue, we analyzed the late response of the transcriptome of the phyA mutant to red light. Compared to the wild-type [WT], hyper-responsive genes outnumbered the genes showing reduced response to red light in phyA. A network analysis revealed the co-expression of PHYTOCHROME INTERACTING FACTOR 1 [PIF1] with those genes showing hyper-promotion by red light in phyA. The enhanced responses of gene expression, cotyledon unfolding, hypocotyl growth, and greening observed in the phyA mutant compared to the WT were absent in the phyA pif1 double mutant compared to pif1, indicating that the hyper-photomorphogenic phenotype of phyA requires PIF1. PIF1 directly binds to gene promoters that displayed PIF1-mediated enhanced response to red light. Expression of mutant PIF1 deficient in interactions with phyA and phyB enhanced the long-term growth response to red light but reduced the expression of selected genes in response to red light. We propose that phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis during early seedling development.SEEDLINGRADIATION RESPONSEPROTEOLYSISPROTEIN TERTIARY STRUCTUREPROTEIN STRUCTURE, TERTIARYPROTEIN STABILITYPROTEIN DEGRADATIONPIF1 PROTEIN, ARABIDOPSISPIF1PHYTOCHROME BPHYTOCHROME APHYAMUTATIONMETABOLISMLIGHT [GROWTH]LIGHT [GENE EXPRESSION]LIGHTGROWTH, DEVELOPMENT AND AGINGGENETICSGENE EXPRESSION REGULATION, PLANTGENE EXPRESSION REGULATIONDE-ETIOLATIONCHEMISTRYBASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORSARABIDOPSIS PROTEINSARABIDOPSISMolecular Plant |
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SEEDLING RADIATION RESPONSE PROTEOLYSIS PROTEIN TERTIARY STRUCTURE PROTEIN STRUCTURE, TERTIARY PROTEIN STABILITY PROTEIN DEGRADATION PIF1 PROTEIN, ARABIDOPSIS PIF1 PHYTOCHROME B PHYTOCHROME A PHYA MUTATION METABOLISM LIGHT [GROWTH] LIGHT [GENE EXPRESSION] LIGHT GROWTH, DEVELOPMENT AND AGING GENETICS GENE EXPRESSION REGULATION, PLANT GENE EXPRESSION REGULATION DE-ETIOLATION CHEMISTRY BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS ARABIDOPSIS PROTEINS ARABIDOPSIS SEEDLING RADIATION RESPONSE PROTEOLYSIS PROTEIN TERTIARY STRUCTURE PROTEIN STRUCTURE, TERTIARY PROTEIN STABILITY PROTEIN DEGRADATION PIF1 PROTEIN, ARABIDOPSIS PIF1 PHYTOCHROME B PHYTOCHROME A PHYA MUTATION METABOLISM LIGHT [GROWTH] LIGHT [GENE EXPRESSION] LIGHT GROWTH, DEVELOPMENT AND AGING GENETICS GENE EXPRESSION REGULATION, PLANT GENE EXPRESSION REGULATION DE-ETIOLATION CHEMISTRY BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS ARABIDOPSIS PROTEINS ARABIDOPSIS |
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SEEDLING RADIATION RESPONSE PROTEOLYSIS PROTEIN TERTIARY STRUCTURE PROTEIN STRUCTURE, TERTIARY PROTEIN STABILITY PROTEIN DEGRADATION PIF1 PROTEIN, ARABIDOPSIS PIF1 PHYTOCHROME B PHYTOCHROME A PHYA MUTATION METABOLISM LIGHT [GROWTH] LIGHT [GENE EXPRESSION] LIGHT GROWTH, DEVELOPMENT AND AGING GENETICS GENE EXPRESSION REGULATION, PLANT GENE EXPRESSION REGULATION DE-ETIOLATION CHEMISTRY BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS ARABIDOPSIS PROTEINS ARABIDOPSIS SEEDLING RADIATION RESPONSE PROTEOLYSIS PROTEIN TERTIARY STRUCTURE PROTEIN STRUCTURE, TERTIARY PROTEIN STABILITY PROTEIN DEGRADATION PIF1 PROTEIN, ARABIDOPSIS PIF1 PHYTOCHROME B PHYTOCHROME A PHYA MUTATION METABOLISM LIGHT [GROWTH] LIGHT [GENE EXPRESSION] LIGHT GROWTH, DEVELOPMENT AND AGING GENETICS GENE EXPRESSION REGULATION, PLANT GENE EXPRESSION REGULATION DE-ETIOLATION CHEMISTRY BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS ARABIDOPSIS PROTEINS ARABIDOPSIS Krzymuski, Martín Cerdán, Pablo D. Zhu, L. Vinh, A. Chory, J. Huq, E. Casal, Jorge José Phytochrome a antagonizes PHYTOCHROME INTERACTING FACTOR 1 to prevent over - activation of photomorphogenesis |
| description |
Phytochrome A [phyA] is crucial to initiate the early steps of the transition between skoto- and photomorphogenesis upon light exposure and to complete this process under far-red light [typical of dense vegetation canopies]. However, under prolonged red or white light, phyA mutants are hyper-photomorphogenic in many respects. To investigate this issue, we analyzed the late response of the transcriptome of the phyA mutant to red light. Compared to the wild-type [WT], hyper-responsive genes outnumbered the genes showing reduced response to red light in phyA. A network analysis revealed the co-expression of PHYTOCHROME INTERACTING FACTOR 1 [PIF1] with those genes showing hyper-promotion by red light in phyA. The enhanced responses of gene expression, cotyledon unfolding, hypocotyl growth, and greening observed in the phyA mutant compared to the WT were absent in the phyA pif1 double mutant compared to pif1, indicating that the hyper-photomorphogenic phenotype of phyA requires PIF1. PIF1 directly binds to gene promoters that displayed PIF1-mediated enhanced response to red light. Expression of mutant PIF1 deficient in interactions with phyA and phyB enhanced the long-term growth response to red light but reduced the expression of selected genes in response to red light. We propose that phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis during early seedling development. |
| format |
Texto |
| topic_facet |
SEEDLING RADIATION RESPONSE PROTEOLYSIS PROTEIN TERTIARY STRUCTURE PROTEIN STRUCTURE, TERTIARY PROTEIN STABILITY PROTEIN DEGRADATION PIF1 PROTEIN, ARABIDOPSIS PIF1 PHYTOCHROME B PHYTOCHROME A PHYA MUTATION METABOLISM LIGHT [GROWTH] LIGHT [GENE EXPRESSION] LIGHT GROWTH, DEVELOPMENT AND AGING GENETICS GENE EXPRESSION REGULATION, PLANT GENE EXPRESSION REGULATION DE-ETIOLATION CHEMISTRY BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS ARABIDOPSIS PROTEINS ARABIDOPSIS |
| author |
Krzymuski, Martín Cerdán, Pablo D. Zhu, L. Vinh, A. Chory, J. Huq, E. Casal, Jorge José |
| author_facet |
Krzymuski, Martín Cerdán, Pablo D. Zhu, L. Vinh, A. Chory, J. Huq, E. Casal, Jorge José |
| author_sort |
Krzymuski, Martín |
| title |
Phytochrome a antagonizes PHYTOCHROME INTERACTING FACTOR 1 to prevent over - activation of photomorphogenesis |
| title_short |
Phytochrome a antagonizes PHYTOCHROME INTERACTING FACTOR 1 to prevent over - activation of photomorphogenesis |
| title_full |
Phytochrome a antagonizes PHYTOCHROME INTERACTING FACTOR 1 to prevent over - activation of photomorphogenesis |
| title_fullStr |
Phytochrome a antagonizes PHYTOCHROME INTERACTING FACTOR 1 to prevent over - activation of photomorphogenesis |
| title_full_unstemmed |
Phytochrome a antagonizes PHYTOCHROME INTERACTING FACTOR 1 to prevent over - activation of photomorphogenesis |
| title_sort |
phytochrome a antagonizes phytochrome interacting factor 1 to prevent over - activation of photomorphogenesis |
| url |
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47214 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
| work_keys_str_mv |
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| _version_ |
1756046747268808704 |