Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5
Fusaric acid is produced by pathogenic fungi of the genus Fusarium, and is toxic to plants and rhizobacteria. Many fluorescent pseudomonads can prevent wilt diseases caused by these fungi. This study was undertaken to evaluate the effect of fusaric acid on P. protegens Pf-5 and elucidate the mechanisms that enable the bacterium to survive in the presence of the mycotoxin. The results confirm that fusaric acid negatively affects growth and motility of P. protegens. Moreover, a notable increase in secretion of the siderophore pyoverdine was observed when P. protegens was grown in the presence of fusaric acid. Concomitantly, levels of enzymes involved in the biosynthesis of pyoverdine and enantio-pyochelin, the second siderophore encoded by P. protegens, increased markedly. Moreover, while similar levels of resistance to fusaric acid were observed for P. protegens mutants unable to synthesize either pyoverdine or enanto-pyochelin and the wild type strain, a double mutant unable to synthesize both kinds of siderophores showed a dramatically reduced resistance to this compound. This reduced resistance was not observed when this mutant was grown under conditions of iron excess. Spectrophotometric titrations revealed that fusaric acid binds not only Fe2+ and Fe3+, but also Zn2+, Mn2+ and Cu2+, with high affinity. Our results demonstrate that iron sequestration accounts at least in part for the deleterious effect of the mycotoxin on P. protegens.
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KOHA-OAI-AGRO:471852022-02-08T13:56:06Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47185http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGProduction of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5Ruiz, J. A.Bernar, E. M.Jung, K.textspaapplication/pdfFusaric acid is produced by pathogenic fungi of the genus Fusarium, and is toxic to plants and rhizobacteria. Many fluorescent pseudomonads can prevent wilt diseases caused by these fungi. This study was undertaken to evaluate the effect of fusaric acid on P. protegens Pf-5 and elucidate the mechanisms that enable the bacterium to survive in the presence of the mycotoxin. The results confirm that fusaric acid negatively affects growth and motility of P. protegens. Moreover, a notable increase in secretion of the siderophore pyoverdine was observed when P. protegens was grown in the presence of fusaric acid. Concomitantly, levels of enzymes involved in the biosynthesis of pyoverdine and enantio-pyochelin, the second siderophore encoded by P. protegens, increased markedly. Moreover, while similar levels of resistance to fusaric acid were observed for P. protegens mutants unable to synthesize either pyoverdine or enanto-pyochelin and the wild type strain, a double mutant unable to synthesize both kinds of siderophores showed a dramatically reduced resistance to this compound. This reduced resistance was not observed when this mutant was grown under conditions of iron excess. Spectrophotometric titrations revealed that fusaric acid binds not only Fe2+ and Fe3+, but also Zn2+, Mn2+ and Cu2+, with high affinity. Our results demonstrate that iron sequestration accounts at least in part for the deleterious effect of the mycotoxin on P. protegens.Fusaric acid is produced by pathogenic fungi of the genus Fusarium, and is toxic to plants and rhizobacteria. Many fluorescent pseudomonads can prevent wilt diseases caused by these fungi. This study was undertaken to evaluate the effect of fusaric acid on P. protegens Pf-5 and elucidate the mechanisms that enable the bacterium to survive in the presence of the mycotoxin. The results confirm that fusaric acid negatively affects growth and motility of P. protegens. Moreover, a notable increase in secretion of the siderophore pyoverdine was observed when P. protegens was grown in the presence of fusaric acid. Concomitantly, levels of enzymes involved in the biosynthesis of pyoverdine and enantio-pyochelin, the second siderophore encoded by P. protegens, increased markedly. Moreover, while similar levels of resistance to fusaric acid were observed for P. protegens mutants unable to synthesize either pyoverdine or enanto-pyochelin and the wild type strain, a double mutant unable to synthesize both kinds of siderophores showed a dramatically reduced resistance to this compound. This reduced resistance was not observed when this mutant was grown under conditions of iron excess. Spectrophotometric titrations revealed that fusaric acid binds not only Fe2+ and Fe3+, but also Zn2+, Mn2+ and Cu2+, with high affinity. Our results demonstrate that iron sequestration accounts at least in part for the deleterious effect of the mycotoxin on P. protegens.ZINC IONWILD TYPEUNCLASSIFIED DRUGSPECTROPHOTOMETRYSIDEROPHORERHIZOBIALESPYOVERDINEPSEUDOMONAS PROTEGENSPSEUDOMONAS FLUORESCENS GROUPPSEUDOMONASPROTEIN EXPRESSIONNONHUMANMULTIDRUG RESISTANCE PROTEINMINIMUM INHIBITORY CONCENTRATIONMICROBIAL PRODUCTS NOT CLASSIFIED ELSEWHEREMANGANESEGROWTH INHIBITIONGENE EXPRESSIONFUSARIUMFUSARIC ACIDFUS GENEFUNGIFERROUS IONFERRIC IONENANTIO PYOCHELINDRUG BINDINGCOPPER IONCONTROLLED STUDYCONCENTRATION RESPONSECELL MOTILITYBIOSYNTHESISBIOFILMBINDING KINETICSBINDING AFFINITYBACTERIUM MUTANTBACTERIUM CULTUREBACTERIAL SURVIVALBACTERIAL STRAINBACTERIAL SECRETION SYSTEMBACTERIAL GROWTHBACTERIAL GENEBACTERIA [MICROORGANISMS]ANTIBIOTIC RESISTANCEANTIBACTERIAL ACTIVITYPlos One |
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ZINC ION WILD TYPE UNCLASSIFIED DRUG SPECTROPHOTOMETRY SIDEROPHORE RHIZOBIALES PYOVERDINE PSEUDOMONAS PROTEGENS PSEUDOMONAS FLUORESCENS GROUP PSEUDOMONAS PROTEIN EXPRESSION NONHUMAN MULTIDRUG RESISTANCE PROTEIN MINIMUM INHIBITORY CONCENTRATION MICROBIAL PRODUCTS NOT CLASSIFIED ELSEWHERE MANGANESE GROWTH INHIBITION GENE EXPRESSION FUSARIUM FUSARIC ACID FUS GENE FUNGI FERROUS ION FERRIC ION ENANTIO PYOCHELIN DRUG BINDING COPPER ION CONTROLLED STUDY CONCENTRATION RESPONSE CELL MOTILITY BIOSYNTHESIS BIOFILM BINDING KINETICS BINDING AFFINITY BACTERIUM MUTANT BACTERIUM CULTURE BACTERIAL SURVIVAL BACTERIAL STRAIN BACTERIAL SECRETION SYSTEM BACTERIAL GROWTH BACTERIAL GENE BACTERIA [MICROORGANISMS] ANTIBIOTIC RESISTANCE ANTIBACTERIAL ACTIVITY ZINC ION WILD TYPE UNCLASSIFIED DRUG SPECTROPHOTOMETRY SIDEROPHORE RHIZOBIALES PYOVERDINE PSEUDOMONAS PROTEGENS PSEUDOMONAS FLUORESCENS GROUP PSEUDOMONAS PROTEIN EXPRESSION NONHUMAN MULTIDRUG RESISTANCE PROTEIN MINIMUM INHIBITORY CONCENTRATION MICROBIAL PRODUCTS NOT CLASSIFIED ELSEWHERE MANGANESE GROWTH INHIBITION GENE EXPRESSION FUSARIUM FUSARIC ACID FUS GENE FUNGI FERROUS ION FERRIC ION ENANTIO PYOCHELIN DRUG BINDING COPPER ION CONTROLLED STUDY CONCENTRATION RESPONSE CELL MOTILITY BIOSYNTHESIS BIOFILM BINDING KINETICS BINDING AFFINITY BACTERIUM MUTANT BACTERIUM CULTURE BACTERIAL SURVIVAL BACTERIAL STRAIN BACTERIAL SECRETION SYSTEM BACTERIAL GROWTH BACTERIAL GENE BACTERIA [MICROORGANISMS] ANTIBIOTIC RESISTANCE ANTIBACTERIAL ACTIVITY |
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ZINC ION WILD TYPE UNCLASSIFIED DRUG SPECTROPHOTOMETRY SIDEROPHORE RHIZOBIALES PYOVERDINE PSEUDOMONAS PROTEGENS PSEUDOMONAS FLUORESCENS GROUP PSEUDOMONAS PROTEIN EXPRESSION NONHUMAN MULTIDRUG RESISTANCE PROTEIN MINIMUM INHIBITORY CONCENTRATION MICROBIAL PRODUCTS NOT CLASSIFIED ELSEWHERE MANGANESE GROWTH INHIBITION GENE EXPRESSION FUSARIUM FUSARIC ACID FUS GENE FUNGI FERROUS ION FERRIC ION ENANTIO PYOCHELIN DRUG BINDING COPPER ION CONTROLLED STUDY CONCENTRATION RESPONSE CELL MOTILITY BIOSYNTHESIS BIOFILM BINDING KINETICS BINDING AFFINITY BACTERIUM MUTANT BACTERIUM CULTURE BACTERIAL SURVIVAL BACTERIAL STRAIN BACTERIAL SECRETION SYSTEM BACTERIAL GROWTH BACTERIAL GENE BACTERIA [MICROORGANISMS] ANTIBIOTIC RESISTANCE ANTIBACTERIAL ACTIVITY ZINC ION WILD TYPE UNCLASSIFIED DRUG SPECTROPHOTOMETRY SIDEROPHORE RHIZOBIALES PYOVERDINE PSEUDOMONAS PROTEGENS PSEUDOMONAS FLUORESCENS GROUP PSEUDOMONAS PROTEIN EXPRESSION NONHUMAN MULTIDRUG RESISTANCE PROTEIN MINIMUM INHIBITORY CONCENTRATION MICROBIAL PRODUCTS NOT CLASSIFIED ELSEWHERE MANGANESE GROWTH INHIBITION GENE EXPRESSION FUSARIUM FUSARIC ACID FUS GENE FUNGI FERROUS ION FERRIC ION ENANTIO PYOCHELIN DRUG BINDING COPPER ION CONTROLLED STUDY CONCENTRATION RESPONSE CELL MOTILITY BIOSYNTHESIS BIOFILM BINDING KINETICS BINDING AFFINITY BACTERIUM MUTANT BACTERIUM CULTURE BACTERIAL SURVIVAL BACTERIAL STRAIN BACTERIAL SECRETION SYSTEM BACTERIAL GROWTH BACTERIAL GENE BACTERIA [MICROORGANISMS] ANTIBIOTIC RESISTANCE ANTIBACTERIAL ACTIVITY Ruiz, J. A. Bernar, E. M. Jung, K. Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| description |
Fusaric acid is produced by pathogenic fungi of the genus Fusarium, and is toxic to plants and rhizobacteria. Many fluorescent pseudomonads can prevent wilt diseases caused by these fungi. This study was undertaken to evaluate the effect of fusaric acid on P. protegens Pf-5 and elucidate the mechanisms that enable the bacterium to survive in the presence of the mycotoxin. The results confirm that fusaric acid negatively affects growth and motility of P. protegens. Moreover, a notable increase in secretion of the siderophore pyoverdine was observed when P. protegens was grown in the presence of fusaric acid. Concomitantly, levels of enzymes involved in the biosynthesis of pyoverdine and enantio-pyochelin, the second siderophore encoded by P. protegens, increased markedly. Moreover, while similar levels of resistance to fusaric acid were observed for P. protegens mutants unable to synthesize either pyoverdine or enanto-pyochelin and the wild type strain, a double mutant unable to synthesize both kinds of siderophores showed a dramatically reduced resistance to this compound. This reduced resistance was not observed when this mutant was grown under conditions of iron excess. Spectrophotometric titrations revealed that fusaric acid binds not only Fe2+ and Fe3+, but also Zn2+, Mn2+ and Cu2+, with high affinity. Our results demonstrate that iron sequestration accounts at least in part for the deleterious effect of the mycotoxin on P. protegens. |
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ZINC ION WILD TYPE UNCLASSIFIED DRUG SPECTROPHOTOMETRY SIDEROPHORE RHIZOBIALES PYOVERDINE PSEUDOMONAS PROTEGENS PSEUDOMONAS FLUORESCENS GROUP PSEUDOMONAS PROTEIN EXPRESSION NONHUMAN MULTIDRUG RESISTANCE PROTEIN MINIMUM INHIBITORY CONCENTRATION MICROBIAL PRODUCTS NOT CLASSIFIED ELSEWHERE MANGANESE GROWTH INHIBITION GENE EXPRESSION FUSARIUM FUSARIC ACID FUS GENE FUNGI FERROUS ION FERRIC ION ENANTIO PYOCHELIN DRUG BINDING COPPER ION CONTROLLED STUDY CONCENTRATION RESPONSE CELL MOTILITY BIOSYNTHESIS BIOFILM BINDING KINETICS BINDING AFFINITY BACTERIUM MUTANT BACTERIUM CULTURE BACTERIAL SURVIVAL BACTERIAL STRAIN BACTERIAL SECRETION SYSTEM BACTERIAL GROWTH BACTERIAL GENE BACTERIA [MICROORGANISMS] ANTIBIOTIC RESISTANCE ANTIBACTERIAL ACTIVITY |
| author |
Ruiz, J. A. Bernar, E. M. Jung, K. |
| author_facet |
Ruiz, J. A. Bernar, E. M. Jung, K. |
| author_sort |
Ruiz, J. A. |
| title |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_short |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_full |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_fullStr |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_full_unstemmed |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_sort |
production of siderophores increases resistance to fusaric acid in pseudomonas protegens pf-5 |
| url |
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47185 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
| work_keys_str_mv |
AT ruizja productionofsiderophoresincreasesresistancetofusaricacidinpseudomonasprotegenspf5 AT bernarem productionofsiderophoresincreasesresistancetofusaricacidinpseudomonasprotegenspf5 AT jungk productionofsiderophoresincreasesresistancetofusaricacidinpseudomonasprotegenspf5 |
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