Nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiation
Antioxidant enzymes play a key role in plant tolerance to different types of stress, including ultraviolet-B [UV-B] radiation. Here we report that nitric oxide [NO] enhances antioxidant enzymes gene expression and increases the activity of specific isoforms protecting against UV-B radiation. Pre-treatments with sodium nitroprussiate [SNP], a NO-donor, prevented lipid peroxidation, ion leakage and H2O2 and superoxide anion accumulation in leaves of UV-B-treated soybean plants. Transcripts levels of superoxide dismutase [SOD], catalase [CAT] and ascorbate peroxidase [APX] were significantly induced by SNP. These data correlated with the enhancement of particular antioxidant enzyme isoforms, such as one CAT isoform and two APX isoforms. Moreover, SNP induced the expression of three new isoforms of SOD, identified as Mn-SOD subclass. Further results showed that total activities of SOD, CAT and APX significantly increased by 2.2-, 1.8- and 2.1-fold in SNP-treated plants compared to controls, respectively. The protective effect of SNP against UV-B radiation was negated by addition of the specific NO scavenger cPTIO, indicating that NO released by SNP mediates the enhancement of antioxidant enzymes activities. In conclusion, NO is involved in the signaling pathway that up-regulates specific isoforms of antioxidant enzymes protecting against UV-B-induced oxidative stress.
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| Subjects: | ASCORBATE PEROXIDASE, CATALASE, CONTROLLED STUDY, ENZYME ACTIVATION, ENZYME ACTIVITY, ENZYME INDUCTION, HYDROGEN PEROXIDE, ION, ION TRANSPORT, LIPID, LIPID PEROXIDATION, MANGANESE SUPEROXIDE DISMUTASE, NITRIC OXIDE, NITROPRUSSIDE SODIUM, NONHUMAN, PEROXIDASE, PLANT DEFENSE, PLANT LEAF, RADIATION PROTECTION, SOYBEAN, SUPEROXIDE, SUPEROXIDE DISMUTASE, ULTRAVIOLET B RADIATION, |
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KOHA-OAI-AGRO:471332022-05-04T11:19:55Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47133http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGNitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiationSanta Cruz, Diego MarioPacienza, NataliaZilli, Carla G.Tomaro, María L.Balestrasse, Karina BeatrizYannarelli, Gustavo G.textspaapplication/pdfAntioxidant enzymes play a key role in plant tolerance to different types of stress, including ultraviolet-B [UV-B] radiation. Here we report that nitric oxide [NO] enhances antioxidant enzymes gene expression and increases the activity of specific isoforms protecting against UV-B radiation. Pre-treatments with sodium nitroprussiate [SNP], a NO-donor, prevented lipid peroxidation, ion leakage and H2O2 and superoxide anion accumulation in leaves of UV-B-treated soybean plants. Transcripts levels of superoxide dismutase [SOD], catalase [CAT] and ascorbate peroxidase [APX] were significantly induced by SNP. These data correlated with the enhancement of particular antioxidant enzyme isoforms, such as one CAT isoform and two APX isoforms. Moreover, SNP induced the expression of three new isoforms of SOD, identified as Mn-SOD subclass. Further results showed that total activities of SOD, CAT and APX significantly increased by 2.2-, 1.8- and 2.1-fold in SNP-treated plants compared to controls, respectively. The protective effect of SNP against UV-B radiation was negated by addition of the specific NO scavenger cPTIO, indicating that NO released by SNP mediates the enhancement of antioxidant enzymes activities. In conclusion, NO is involved in the signaling pathway that up-regulates specific isoforms of antioxidant enzymes protecting against UV-B-induced oxidative stress.Antioxidant enzymes play a key role in plant tolerance to different types of stress, including ultraviolet-B [UV-B] radiation. Here we report that nitric oxide [NO] enhances antioxidant enzymes gene expression and increases the activity of specific isoforms protecting against UV-B radiation. Pre-treatments with sodium nitroprussiate [SNP], a NO-donor, prevented lipid peroxidation, ion leakage and H2O2 and superoxide anion accumulation in leaves of UV-B-treated soybean plants. Transcripts levels of superoxide dismutase [SOD], catalase [CAT] and ascorbate peroxidase [APX] were significantly induced by SNP. These data correlated with the enhancement of particular antioxidant enzyme isoforms, such as one CAT isoform and two APX isoforms. Moreover, SNP induced the expression of three new isoforms of SOD, identified as Mn-SOD subclass. Further results showed that total activities of SOD, CAT and APX significantly increased by 2.2-, 1.8- and 2.1-fold in SNP-treated plants compared to controls, respectively. The protective effect of SNP against UV-B radiation was negated by addition of the specific NO scavenger cPTIO, indicating that NO released by SNP mediates the enhancement of antioxidant enzymes activities. In conclusion, NO is involved in the signaling pathway that up-regulates specific isoforms of antioxidant enzymes protecting against UV-B-induced oxidative stress.ASCORBATE PEROXIDASECATALASECONTROLLED STUDYENZYME ACTIVATIONENZYME ACTIVITYENZYME INDUCTIONHYDROGEN PEROXIDEIONION TRANSPORTLIPIDLIPID PEROXIDATIONMANGANESE SUPEROXIDE DISMUTASENITRIC OXIDENITROPRUSSIDE SODIUMNONHUMANPEROXIDASEPLANT DEFENSEPLANT LEAFRADIATION PROTECTIONSOYBEANSUPEROXIDESUPEROXIDE DISMUTASEULTRAVIOLET B RADIATIONJournal of Photochemistry and Photobiology B: Biology |
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ASCORBATE PEROXIDASE CATALASE CONTROLLED STUDY ENZYME ACTIVATION ENZYME ACTIVITY ENZYME INDUCTION HYDROGEN PEROXIDE ION ION TRANSPORT LIPID LIPID PEROXIDATION MANGANESE SUPEROXIDE DISMUTASE NITRIC OXIDE NITROPRUSSIDE SODIUM NONHUMAN PEROXIDASE PLANT DEFENSE PLANT LEAF RADIATION PROTECTION SOYBEAN SUPEROXIDE SUPEROXIDE DISMUTASE ULTRAVIOLET B RADIATION ASCORBATE PEROXIDASE CATALASE CONTROLLED STUDY ENZYME ACTIVATION ENZYME ACTIVITY ENZYME INDUCTION HYDROGEN PEROXIDE ION ION TRANSPORT LIPID LIPID PEROXIDATION MANGANESE SUPEROXIDE DISMUTASE NITRIC OXIDE NITROPRUSSIDE SODIUM NONHUMAN PEROXIDASE PLANT DEFENSE PLANT LEAF RADIATION PROTECTION SOYBEAN SUPEROXIDE SUPEROXIDE DISMUTASE ULTRAVIOLET B RADIATION |
| spellingShingle |
ASCORBATE PEROXIDASE CATALASE CONTROLLED STUDY ENZYME ACTIVATION ENZYME ACTIVITY ENZYME INDUCTION HYDROGEN PEROXIDE ION ION TRANSPORT LIPID LIPID PEROXIDATION MANGANESE SUPEROXIDE DISMUTASE NITRIC OXIDE NITROPRUSSIDE SODIUM NONHUMAN PEROXIDASE PLANT DEFENSE PLANT LEAF RADIATION PROTECTION SOYBEAN SUPEROXIDE SUPEROXIDE DISMUTASE ULTRAVIOLET B RADIATION ASCORBATE PEROXIDASE CATALASE CONTROLLED STUDY ENZYME ACTIVATION ENZYME ACTIVITY ENZYME INDUCTION HYDROGEN PEROXIDE ION ION TRANSPORT LIPID LIPID PEROXIDATION MANGANESE SUPEROXIDE DISMUTASE NITRIC OXIDE NITROPRUSSIDE SODIUM NONHUMAN PEROXIDASE PLANT DEFENSE PLANT LEAF RADIATION PROTECTION SOYBEAN SUPEROXIDE SUPEROXIDE DISMUTASE ULTRAVIOLET B RADIATION Santa Cruz, Diego Mario Pacienza, Natalia Zilli, Carla G. Tomaro, María L. Balestrasse, Karina Beatriz Yannarelli, Gustavo G. Nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiation |
| description |
Antioxidant enzymes play a key role in plant tolerance to different types of stress, including ultraviolet-B [UV-B] radiation. Here we report that nitric oxide [NO] enhances antioxidant enzymes gene expression and increases the activity of specific isoforms protecting against UV-B radiation. Pre-treatments with sodium nitroprussiate [SNP], a NO-donor, prevented lipid peroxidation, ion leakage and H2O2 and superoxide anion accumulation in leaves of UV-B-treated soybean plants. Transcripts levels of superoxide dismutase [SOD], catalase [CAT] and ascorbate peroxidase [APX] were significantly induced by SNP. These data correlated with the enhancement of particular antioxidant enzyme isoforms, such as one CAT isoform and two APX isoforms. Moreover, SNP induced the expression of three new isoforms of SOD, identified as Mn-SOD subclass. Further results showed that total activities of SOD, CAT and APX significantly increased by 2.2-, 1.8- and 2.1-fold in SNP-treated plants compared to controls, respectively. The protective effect of SNP against UV-B radiation was negated by addition of the specific NO scavenger cPTIO, indicating that NO released by SNP mediates the enhancement of antioxidant enzymes activities. In conclusion, NO is involved in the signaling pathway that up-regulates specific isoforms of antioxidant enzymes protecting against UV-B-induced oxidative stress. |
| format |
Texto |
| topic_facet |
ASCORBATE PEROXIDASE CATALASE CONTROLLED STUDY ENZYME ACTIVATION ENZYME ACTIVITY ENZYME INDUCTION HYDROGEN PEROXIDE ION ION TRANSPORT LIPID LIPID PEROXIDATION MANGANESE SUPEROXIDE DISMUTASE NITRIC OXIDE NITROPRUSSIDE SODIUM NONHUMAN PEROXIDASE PLANT DEFENSE PLANT LEAF RADIATION PROTECTION SOYBEAN SUPEROXIDE SUPEROXIDE DISMUTASE ULTRAVIOLET B RADIATION |
| author |
Santa Cruz, Diego Mario Pacienza, Natalia Zilli, Carla G. Tomaro, María L. Balestrasse, Karina Beatriz Yannarelli, Gustavo G. |
| author_facet |
Santa Cruz, Diego Mario Pacienza, Natalia Zilli, Carla G. Tomaro, María L. Balestrasse, Karina Beatriz Yannarelli, Gustavo G. |
| author_sort |
Santa Cruz, Diego Mario |
| title |
Nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiation |
| title_short |
Nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiation |
| title_full |
Nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiation |
| title_fullStr |
Nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiation |
| title_full_unstemmed |
Nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiation |
| title_sort |
nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - b radiation |
| url |
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47133 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
| work_keys_str_mv |
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| _version_ |
1756046736136077312 |