Evaluation of indigenous bacterial strains for biocontrol of the frogeye leaf spot of soya bean caused by Cercospora sojina

Aims: Assessment of biological control of Cercospora sojina, causal agent of frogeye leaf spot [FLS] of soya bean, using three indigenous bacterial strains, BNM297 [Pseudomonas fluorescens], BNM340 and BNM122 [Bacillus amyloliquefaciens]. Methods and Results: From cultures of each bacterial strain, cell suspensions and cell-free supernatants were obtained and assayed to determine their antifungal activity against C. sojina. Both mycelial growth and spore germination in vitro were more strongly inhibited by bacterial cell suspensions than by cell-free supernatants. The Bacillus strains BNM122 and BNM340 inhibited the fungal growth to a similar degree [I ~52-53 percent], while cells from P. fluorescens BNM297 caused a lesser reduction [I ~32-34 percent] in the fungus colony diameter. The foliar application of the two Bacillus strains on soya bean seedlings, under greenhouse conditions, significantly reduced the disease severity with respect to control soya bean seedlings and those sprayed with BNM297. This last bacterial strain was not effective in controlling FLS in vivo. Conclusions: Our data demonstrate that the application of antagonistic bacteria may be a promising and environmentally friendly alternative to control the FLS of soya bean. Significance and Impact of the Study: To our knowledge, this is the first report of biological control of C. sojina by using native Bacillus strains.

Bibliographic Details

Main Authors:	Simonetti, Ester, Carmona, Marcelo Aníbal, Scandiani, María Mercedes, García, A. F., Luque, A. G., Correa, Olga Susana, Balestrasse, Karina Beatriz
Format:	Texto biblioteca
Language:	eng
Subjects:	BIOLOGICAL CONTROL, FROGEYE LEAF SPOT, ANTAGONISM, ANTIMICROBIAL ACTIVITY, BACTERIUM, FUNGAL DISEASE, GREENHOUSE ECOSYSTEM, INHIBITION, SEEDLING, ANTIFUNGAL ACTIVITY, BACTERIAL CELL, BACTERIAL STRAIN, BACTERIUM CULTURE, CELL SUSPENSION, CONTROLLED STUDY, DISEASE SEVERITY, FUNGAL SPORE GERMINATION, FUNGUS, GREENHOUSE, IN VITRO STUDY, LEAF LITTER, LEAF SPOT, MYCELIAL GROWTH, NONHUMAN, RHIZOSPHERE FUNGUS, SOYBEAN, SUPERNATANT, ASCOMYCOTA, BACILLUS, PLANT DISEASES, PLANT LEAVES, PSEUDOMONAS FLUORESCENS, SOYBEANS, BACILLUS [BACTERIUM], BACILLUS AMYLOLIQUEFACIENS, BACTERIA [MICROORGANISMS], CERCOSPORA SOJINA, FUNGI,
Online Access:	http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=46802
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