Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis
Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change grather than 2.5 and less than -2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis.
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KOHA-OAI-AGRO:46469 |
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record_format |
koha |
institution |
UBA FA |
collection |
Koha |
country |
Argentina |
countrycode |
AR |
component |
Bibliográfico |
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En linea En linea |
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cat-ceiba |
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biblioteca |
region |
America del Sur |
libraryname |
Biblioteca Central FAUBA |
language |
eng |
topic |
AMPHIREGULIN B BIOLOGICAL MARKER CALGRANULIN B CD14 ANTIGEN CHEMOKINE RECEPTOR CXCR2 CXCL9 CHEMOKINE FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR GELATINASE B HYALURONAN SYNTHASE 2 INTERLEUKIN 1 RECEPTOR INTERLEUKIN 13 RECEPTOR ALPHA1 INTERLEUKIN 22 LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN MANNOSE RECEPTOR C TYPE 1 MATRIX METALLOPROTEINASE 19 MONOCYTE CHEMOTACTIC PROTEIN 2 NEUROPILIN 1 RESISTIN RNA SH3 DOMAIN BINDING PROTEIN 4 THROMBOCYTE FACTOR 4 TRANSCRIPTION FACTOR TRANSCRIPTION FACTOR FYVE TRANSCRIPTION FACTOR GZMA TRANSCRIPTION FACTOR THBS1 TRANSFORMING GROWTH FACTOR BETA TRANSGLUTAMINASE 3 UNCLASSIFIED DRUG UROKINASE RECEPTOR VERY LOW DENSITY LIPOPROTEIN RECEPTOR ADAM8 GENE ADAMTSL4 GENE ANIMAL CELL ANIMAL EXPERIMENT ANIMAL MODEL ANIMAL TISSUE APOPTOSIS AREGB GENE ARHGAP21 GENE BOVINE TUBERCULOSIS CATTLE DISEASE CCL2 GENE CCL8 GENE CCR1 GENE CD14 GENE CELL TRANSPORT CSF3R GENE CXCL10 GENE CXCL9 GENE CXCR2 GENE DEFBIO GENE DOWN REGULATION ECM1 GENE ENDOCYTOSIS FCAR GENE FCGR1A GENE FYVE GENE GENE GENE EXPRESSION PROFILING GENETIC VARIABILITY GZMA GENE HAS2 GENE IL 18 GENE IL 1R GENE IL 6 GENE IL13RA1 GENE IL1R1 GENE IL1RN GENE IL22 GENE IMMUNE RESPONSE INFLAMMATION LOC508666 GENE LOC533894 GENE MAPK13 GENE MAPK14 GENE MICROARRAY ANALYSIS MMP19 GENE MMP3 GENE MMP9 GENE MRC1 GENE MYCOBACTERIUM BOVIS MYD88 GENE NFAM1 GENE NONHUMAN NRP1 GENE PERIPHERAL BLOOD MONONUCLEAR CELL PF4 GENE PLAUR GENE PROTEIN DEGRADATION PYCARD GENE QUANTITATIVE ANALYSIS RETN GENE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION S100A9 GENE SASH1 GENE SH3BP4 GENE TGFBI GENE TGM3 GENE THBS1 GENE TREM1 GENE TYROBP GENE UPREGULATION VLDLR GENE ANIMALS BIOLOGICAL MARKERS CATTLE ENDOCYTOSIS GENE EXPRESSION PROFILING GENE EXPRESSION REGULATION, BACTERIAL INFLAMMATION LEUKOCYTES, MONONUCLEAR MODELS, GENETIC MYCOBACTERIUM BOVIS OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS TRANSCRIPTION, GENETIC TUBERCULOSIS, BOVINE BOS BOVINAE MYCOBACTERIUM BOVIS AMPHIREGULIN B BIOLOGICAL MARKER CALGRANULIN B CD14 ANTIGEN CHEMOKINE RECEPTOR CXCR2 CXCL9 CHEMOKINE FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR GELATINASE B HYALURONAN SYNTHASE 2 INTERLEUKIN 1 RECEPTOR INTERLEUKIN 13 RECEPTOR ALPHA1 INTERLEUKIN 22 LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN MANNOSE RECEPTOR C TYPE 1 MATRIX METALLOPROTEINASE 19 MONOCYTE CHEMOTACTIC PROTEIN 2 NEUROPILIN 1 RESISTIN RNA SH3 DOMAIN BINDING PROTEIN 4 THROMBOCYTE FACTOR 4 TRANSCRIPTION FACTOR TRANSCRIPTION FACTOR FYVE TRANSCRIPTION FACTOR GZMA TRANSCRIPTION FACTOR THBS1 TRANSFORMING GROWTH FACTOR BETA TRANSGLUTAMINASE 3 UNCLASSIFIED DRUG UROKINASE RECEPTOR VERY LOW DENSITY LIPOPROTEIN RECEPTOR ADAM8 GENE ADAMTSL4 GENE ANIMAL CELL ANIMAL EXPERIMENT ANIMAL MODEL ANIMAL TISSUE APOPTOSIS AREGB GENE ARHGAP21 GENE BOVINE TUBERCULOSIS CATTLE DISEASE CCL2 GENE CCL8 GENE CCR1 GENE CD14 GENE CELL TRANSPORT CSF3R GENE CXCL10 GENE CXCL9 GENE CXCR2 GENE DEFBIO GENE DOWN REGULATION ECM1 GENE ENDOCYTOSIS FCAR GENE FCGR1A GENE FYVE GENE GENE GENE EXPRESSION PROFILING GENETIC VARIABILITY GZMA GENE HAS2 GENE IL 18 GENE IL 1R GENE IL 6 GENE IL13RA1 GENE IL1R1 GENE IL1RN GENE IL22 GENE IMMUNE RESPONSE INFLAMMATION LOC508666 GENE LOC533894 GENE MAPK13 GENE MAPK14 GENE MICROARRAY ANALYSIS MMP19 GENE MMP3 GENE MMP9 GENE MRC1 GENE MYCOBACTERIUM BOVIS MYD88 GENE NFAM1 GENE NONHUMAN NRP1 GENE PERIPHERAL BLOOD MONONUCLEAR CELL PF4 GENE PLAUR GENE PROTEIN DEGRADATION PYCARD GENE QUANTITATIVE ANALYSIS RETN GENE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION S100A9 GENE SASH1 GENE SH3BP4 GENE TGFBI GENE TGM3 GENE THBS1 GENE TREM1 GENE TYROBP GENE UPREGULATION VLDLR GENE ANIMALS BIOLOGICAL MARKERS CATTLE ENDOCYTOSIS GENE EXPRESSION PROFILING GENE EXPRESSION REGULATION, BACTERIAL INFLAMMATION LEUKOCYTES, MONONUCLEAR MODELS, GENETIC MYCOBACTERIUM BOVIS OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS TRANSCRIPTION, GENETIC TUBERCULOSIS, BOVINE BOS BOVINAE MYCOBACTERIUM BOVIS |
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AMPHIREGULIN B BIOLOGICAL MARKER CALGRANULIN B CD14 ANTIGEN CHEMOKINE RECEPTOR CXCR2 CXCL9 CHEMOKINE FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR GELATINASE B HYALURONAN SYNTHASE 2 INTERLEUKIN 1 RECEPTOR INTERLEUKIN 13 RECEPTOR ALPHA1 INTERLEUKIN 22 LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN MANNOSE RECEPTOR C TYPE 1 MATRIX METALLOPROTEINASE 19 MONOCYTE CHEMOTACTIC PROTEIN 2 NEUROPILIN 1 RESISTIN RNA SH3 DOMAIN BINDING PROTEIN 4 THROMBOCYTE FACTOR 4 TRANSCRIPTION FACTOR TRANSCRIPTION FACTOR FYVE TRANSCRIPTION FACTOR GZMA TRANSCRIPTION FACTOR THBS1 TRANSFORMING GROWTH FACTOR BETA TRANSGLUTAMINASE 3 UNCLASSIFIED DRUG UROKINASE RECEPTOR VERY LOW DENSITY LIPOPROTEIN RECEPTOR ADAM8 GENE ADAMTSL4 GENE ANIMAL CELL ANIMAL EXPERIMENT ANIMAL MODEL ANIMAL TISSUE APOPTOSIS AREGB GENE ARHGAP21 GENE BOVINE TUBERCULOSIS CATTLE DISEASE CCL2 GENE CCL8 GENE CCR1 GENE CD14 GENE CELL TRANSPORT CSF3R GENE CXCL10 GENE CXCL9 GENE CXCR2 GENE DEFBIO GENE DOWN REGULATION ECM1 GENE ENDOCYTOSIS FCAR GENE FCGR1A GENE FYVE GENE GENE GENE EXPRESSION PROFILING GENETIC VARIABILITY GZMA GENE HAS2 GENE IL 18 GENE IL 1R GENE IL 6 GENE IL13RA1 GENE IL1R1 GENE IL1RN GENE IL22 GENE IMMUNE RESPONSE INFLAMMATION LOC508666 GENE LOC533894 GENE MAPK13 GENE MAPK14 GENE MICROARRAY ANALYSIS MMP19 GENE MMP3 GENE MMP9 GENE MRC1 GENE MYCOBACTERIUM BOVIS MYD88 GENE NFAM1 GENE NONHUMAN NRP1 GENE PERIPHERAL BLOOD MONONUCLEAR CELL PF4 GENE PLAUR GENE PROTEIN DEGRADATION PYCARD GENE QUANTITATIVE ANALYSIS RETN GENE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION S100A9 GENE SASH1 GENE SH3BP4 GENE TGFBI GENE TGM3 GENE THBS1 GENE TREM1 GENE TYROBP GENE UPREGULATION VLDLR GENE ANIMALS BIOLOGICAL MARKERS CATTLE ENDOCYTOSIS GENE EXPRESSION PROFILING GENE EXPRESSION REGULATION, BACTERIAL INFLAMMATION LEUKOCYTES, MONONUCLEAR MODELS, GENETIC MYCOBACTERIUM BOVIS OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS TRANSCRIPTION, GENETIC TUBERCULOSIS, BOVINE BOS BOVINAE MYCOBACTERIUM BOVIS AMPHIREGULIN B BIOLOGICAL MARKER CALGRANULIN B CD14 ANTIGEN CHEMOKINE RECEPTOR CXCR2 CXCL9 CHEMOKINE FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR GELATINASE B HYALURONAN SYNTHASE 2 INTERLEUKIN 1 RECEPTOR INTERLEUKIN 13 RECEPTOR ALPHA1 INTERLEUKIN 22 LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN MANNOSE RECEPTOR C TYPE 1 MATRIX METALLOPROTEINASE 19 MONOCYTE CHEMOTACTIC PROTEIN 2 NEUROPILIN 1 RESISTIN RNA SH3 DOMAIN BINDING PROTEIN 4 THROMBOCYTE FACTOR 4 TRANSCRIPTION FACTOR TRANSCRIPTION FACTOR FYVE TRANSCRIPTION FACTOR GZMA TRANSCRIPTION FACTOR THBS1 TRANSFORMING GROWTH FACTOR BETA TRANSGLUTAMINASE 3 UNCLASSIFIED DRUG UROKINASE RECEPTOR VERY LOW DENSITY LIPOPROTEIN RECEPTOR ADAM8 GENE ADAMTSL4 GENE ANIMAL CELL ANIMAL EXPERIMENT ANIMAL MODEL ANIMAL TISSUE APOPTOSIS AREGB GENE ARHGAP21 GENE BOVINE TUBERCULOSIS CATTLE DISEASE CCL2 GENE CCL8 GENE CCR1 GENE CD14 GENE CELL TRANSPORT CSF3R GENE CXCL10 GENE CXCL9 GENE CXCR2 GENE DEFBIO GENE DOWN REGULATION ECM1 GENE ENDOCYTOSIS FCAR GENE FCGR1A GENE FYVE GENE GENE GENE EXPRESSION PROFILING GENETIC VARIABILITY GZMA GENE HAS2 GENE IL 18 GENE IL 1R GENE IL 6 GENE IL13RA1 GENE IL1R1 GENE IL1RN GENE IL22 GENE IMMUNE RESPONSE INFLAMMATION LOC508666 GENE LOC533894 GENE MAPK13 GENE MAPK14 GENE MICROARRAY ANALYSIS MMP19 GENE MMP3 GENE MMP9 GENE MRC1 GENE MYCOBACTERIUM BOVIS MYD88 GENE NFAM1 GENE NONHUMAN NRP1 GENE PERIPHERAL BLOOD MONONUCLEAR CELL PF4 GENE PLAUR GENE PROTEIN DEGRADATION PYCARD GENE QUANTITATIVE ANALYSIS RETN GENE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION S100A9 GENE SASH1 GENE SH3BP4 GENE TGFBI GENE TGM3 GENE THBS1 GENE TREM1 GENE TYROBP GENE UPREGULATION VLDLR GENE ANIMALS BIOLOGICAL MARKERS CATTLE ENDOCYTOSIS GENE EXPRESSION PROFILING GENE EXPRESSION REGULATION, BACTERIAL INFLAMMATION LEUKOCYTES, MONONUCLEAR MODELS, GENETIC MYCOBACTERIUM BOVIS OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS TRANSCRIPTION, GENETIC TUBERCULOSIS, BOVINE BOS BOVINAE MYCOBACTERIUM BOVIS Blanco, Federico Carlos Soria, M. A. Bianco, María Verónica Bigi, Fabiana Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis |
description |
Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change grather than 2.5 and less than -2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis. |
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topic_facet |
AMPHIREGULIN B BIOLOGICAL MARKER CALGRANULIN B CD14 ANTIGEN CHEMOKINE RECEPTOR CXCR2 CXCL9 CHEMOKINE FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR GELATINASE B HYALURONAN SYNTHASE 2 INTERLEUKIN 1 RECEPTOR INTERLEUKIN 13 RECEPTOR ALPHA1 INTERLEUKIN 22 LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN MANNOSE RECEPTOR C TYPE 1 MATRIX METALLOPROTEINASE 19 MONOCYTE CHEMOTACTIC PROTEIN 2 NEUROPILIN 1 RESISTIN RNA SH3 DOMAIN BINDING PROTEIN 4 THROMBOCYTE FACTOR 4 TRANSCRIPTION FACTOR TRANSCRIPTION FACTOR FYVE TRANSCRIPTION FACTOR GZMA TRANSCRIPTION FACTOR THBS1 TRANSFORMING GROWTH FACTOR BETA TRANSGLUTAMINASE 3 UNCLASSIFIED DRUG UROKINASE RECEPTOR VERY LOW DENSITY LIPOPROTEIN RECEPTOR ADAM8 GENE ADAMTSL4 GENE ANIMAL CELL ANIMAL EXPERIMENT ANIMAL MODEL ANIMAL TISSUE APOPTOSIS AREGB GENE ARHGAP21 GENE BOVINE TUBERCULOSIS CATTLE DISEASE CCL2 GENE CCL8 GENE CCR1 GENE CD14 GENE CELL TRANSPORT CSF3R GENE CXCL10 GENE CXCL9 GENE CXCR2 GENE DEFBIO GENE DOWN REGULATION ECM1 GENE ENDOCYTOSIS FCAR GENE FCGR1A GENE FYVE GENE GENE GENE EXPRESSION PROFILING GENETIC VARIABILITY GZMA GENE HAS2 GENE IL 18 GENE IL 1R GENE IL 6 GENE IL13RA1 GENE IL1R1 GENE IL1RN GENE IL22 GENE IMMUNE RESPONSE INFLAMMATION LOC508666 GENE LOC533894 GENE MAPK13 GENE MAPK14 GENE MICROARRAY ANALYSIS MMP19 GENE MMP3 GENE MMP9 GENE MRC1 GENE MYCOBACTERIUM BOVIS MYD88 GENE NFAM1 GENE NONHUMAN NRP1 GENE PERIPHERAL BLOOD MONONUCLEAR CELL PF4 GENE PLAUR GENE PROTEIN DEGRADATION PYCARD GENE QUANTITATIVE ANALYSIS RETN GENE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION S100A9 GENE SASH1 GENE SH3BP4 GENE TGFBI GENE TGM3 GENE THBS1 GENE TREM1 GENE TYROBP GENE UPREGULATION VLDLR GENE ANIMALS BIOLOGICAL MARKERS CATTLE ENDOCYTOSIS GENE EXPRESSION PROFILING GENE EXPRESSION REGULATION, BACTERIAL INFLAMMATION LEUKOCYTES, MONONUCLEAR MODELS, GENETIC MYCOBACTERIUM BOVIS OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS TRANSCRIPTION, GENETIC TUBERCULOSIS, BOVINE BOS BOVINAE MYCOBACTERIUM BOVIS |
author |
Blanco, Federico Carlos Soria, M. A. Bianco, María Verónica Bigi, Fabiana |
author_facet |
Blanco, Federico Carlos Soria, M. A. Bianco, María Verónica Bigi, Fabiana |
author_sort |
Blanco, Federico Carlos |
title |
Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis |
title_short |
Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis |
title_full |
Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis |
title_fullStr |
Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis |
title_full_unstemmed |
Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis |
title_sort |
transcriptional response of peripheral blood mononuclear cells from cattle infected with mycobacterium bovis |
url |
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=46469 |
work_keys_str_mv |
AT blancofedericocarlos transcriptionalresponseofperipheralbloodmononuclearcellsfromcattleinfectedwithmycobacteriumbovis AT soriama transcriptionalresponseofperipheralbloodmononuclearcellsfromcattleinfectedwithmycobacteriumbovis AT biancomariaveronica transcriptionalresponseofperipheralbloodmononuclearcellsfromcattleinfectedwithmycobacteriumbovis AT bigifabiana transcriptionalresponseofperipheralbloodmononuclearcellsfromcattleinfectedwithmycobacteriumbovis |
_version_ |
1763176938348740608 |
spelling |
KOHA-OAI-AGRO:464692023-04-05T12:38:55Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=46469AAGTranscriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovisBlanco, Federico CarlosSoria, M. A.Bianco, María VerónicaBigi, Fabianatextengapplication/pdfMycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change grather than 2.5 and less than -2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis.Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change grather than 2.5 and less than -2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis.AMPHIREGULIN BBIOLOGICAL MARKERCALGRANULIN BCD14 ANTIGENCHEMOKINE RECEPTOR CXCR2CXCL9 CHEMOKINEFC FRAGMENT IMMUNOGLOBULIN A RECEPTORGELATINASE BHYALURONAN SYNTHASE 2INTERLEUKIN 1 RECEPTORINTERLEUKIN 13 RECEPTOR ALPHA1INTERLEUKIN 22LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEINMANNOSE RECEPTOR C TYPE 1MATRIX METALLOPROTEINASE 19MONOCYTE CHEMOTACTIC PROTEIN 2NEUROPILIN 1RESISTINRNASH3 DOMAIN BINDING PROTEIN 4THROMBOCYTE FACTOR 4TRANSCRIPTION FACTORTRANSCRIPTION FACTOR FYVETRANSCRIPTION FACTOR GZMATRANSCRIPTION FACTOR THBS1TRANSFORMING GROWTH FACTOR BETATRANSGLUTAMINASE 3UNCLASSIFIED DRUGUROKINASE RECEPTORVERY LOW DENSITY LIPOPROTEIN RECEPTORADAM8 GENEADAMTSL4 GENEANIMAL CELLANIMAL EXPERIMENTANIMAL MODELANIMAL TISSUEAPOPTOSISAREGB GENEARHGAP21 GENEBOVINE TUBERCULOSISCATTLE DISEASECCL2 GENECCL8 GENECCR1 GENECD14 GENECELL TRANSPORTCSF3R GENECXCL10 GENECXCL9 GENECXCR2 GENEDEFBIO GENEDOWN REGULATIONECM1 GENEENDOCYTOSISFCAR GENEFCGR1A GENEFYVE GENEGENEGENE EXPRESSION PROFILINGGENETIC VARIABILITYGZMA GENEHAS2 GENEIL 18 GENEIL 1R GENEIL 6 GENEIL13RA1 GENEIL1R1 GENEIL1RN GENEIL22 GENEIMMUNE RESPONSEINFLAMMATIONLOC508666 GENELOC533894 GENEMAPK13 GENEMAPK14 GENEMICROARRAY ANALYSISMMP19 GENEMMP3 GENEMMP9 GENEMRC1 GENEMYCOBACTERIUM BOVISMYD88 GENENFAM1 GENENONHUMANNRP1 GENEPERIPHERAL BLOOD MONONUCLEAR CELLPF4 GENEPLAUR GENEPROTEIN DEGRADATIONPYCARD GENEQUANTITATIVE ANALYSISRETN GENEREVERSE TRANSCRIPTION POLYMERASE CHAIN REACTIONS100A9 GENESASH1 GENESH3BP4 GENETGFBI GENETGM3 GENETHBS1 GENETREM1 GENETYROBP GENEUPREGULATIONVLDLR GENEANIMALSBIOLOGICAL MARKERSCATTLEENDOCYTOSISGENE EXPRESSION PROFILINGGENE EXPRESSION REGULATION, BACTERIALINFLAMMATIONLEUKOCYTES, MONONUCLEARMODELS, GENETICMYCOBACTERIUM BOVISOLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSISTRANSCRIPTION, GENETICTUBERCULOSIS, BOVINEBOSBOVINAEMYCOBACTERIUM BOVISPlos One |