Characterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses

Characterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses

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The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized midgut microbiota of stinkbugs collected from soybean crops, different secondary plant hosts or insects at diapause on Eucalyptus trees. Our study demonstrated that while more than 54% of N. viridula adults collected in the field had no detectable bacteria in the V1-V3 midgut ventricles, the guts of the rest of stinkbugs were colonized by non-transient microbiota (NTM) and transient microbiota not present in stinkbugs at diapause. While transient microbiota Bacillus sp., Micrococcus sp., Streptomyces sp., Staphylococcus sp. and others had low abundance, NTM microbiota was represented by Yokenella sp., Pantoea sp. and Enterococcus sp. isolates. We found some isolates that showed in vitro ß-glucosidase and raffinase activities plus the ability to degrade isoflavonoids and deactivate soybean protease inhibitors. Our results suggest that the stinkbugs NTM microbiota may impact on nutrition, detoxification and deactivation of chemical defenses, and Enterococcus sp., Yokenella sp. and Pantoea sp. strains might help stinkbugs to feed on soybean developing seeds in spite of its chemical defenses.

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Main Authors: Medina, Virginia, Sardoy, Pedro Miguel, Soria, Marcelo Abel, Vay, Carlos A., Gutkind, Gabriel O., Zavala, Jorge Alberto
Format: Texto biblioteca
Language:eng
Subjects:BACTERIAL ENZYME, BETA GLUCOSIDASE, RAFFINASE, UNCLASSIFIED DRUG, BACILLUS, BACTERIUM, BACTERIUM ISOLATE, CONTROLLED STUDY, INSECT, NEZARA VIRIDULA, MICROCOCCUS,
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spelling KOHA-OAI-AGRO:459592023-11-23T15:02:03Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45959http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGCharacterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defensesMedina, VirginiaSardoy, Pedro MiguelSoria, Marcelo AbelVay, Carlos A.Gutkind, Gabriel O.Zavala, Jorge Albertotextengapplication/pdfThe Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized midgut microbiota of stinkbugs collected from soybean crops, different secondary plant hosts or insects at diapause on Eucalyptus trees. Our study demonstrated that while more than 54% of N. viridula adults collected in the field had no detectable bacteria in the V1-V3 midgut ventricles, the guts of the rest of stinkbugs were colonized by non-transient microbiota (NTM) and transient microbiota not present in stinkbugs at diapause. While transient microbiota Bacillus sp., Micrococcus sp., Streptomyces sp., Staphylococcus sp. and others had low abundance, NTM microbiota was represented by Yokenella sp., Pantoea sp. and Enterococcus sp. isolates. We found some isolates that showed in vitro ß-glucosidase and raffinase activities plus the ability to degrade isoflavonoids and deactivate soybean protease inhibitors. Our results suggest that the stinkbugs NTM microbiota may impact on nutrition, detoxification and deactivation of chemical defenses, and Enterococcus sp., Yokenella sp. and Pantoea sp. strains might help stinkbugs to feed on soybean developing seeds in spite of its chemical defenses.The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized midgut microbiota of stinkbugs collected from soybean crops, different secondary plant hosts or insects at diapause on Eucalyptus trees. Our study demonstrated that while more than 54% of N. viridula adults collected in the field had no detectable bacteria in the V1-V3 midgut ventricles, the guts of the rest of stinkbugs were colonized by non-transient microbiota (NTM) and transient microbiota not present in stinkbugs at diapause. While transient microbiota Bacillus sp., Micrococcus sp., Streptomyces sp., Staphylococcus sp. and others had low abundance, NTM microbiota was represented by Yokenella sp., Pantoea sp. and Enterococcus sp. isolates. We found some isolates that showed in vitro ß-glucosidase and raffinase activities plus the ability to degrade isoflavonoids and deactivate soybean protease inhibitors. Our results suggest that the stinkbugs NTM microbiota may impact on nutrition, detoxification and deactivation of chemical defenses, and Enterococcus sp., Yokenella sp. and Pantoea sp. strains might help stinkbugs to feed on soybean developing seeds in spite of its chemical defenses.BACTERIAL ENZYMEBETA GLUCOSIDASERAFFINASEUNCLASSIFIED DRUGBACILLUSBACTERIUMBACTERIUM ISOLATECONTROLLED STUDYINSECTNEZARA VIRIDULAMICROCOCCUSPlos One
institution UBA FA
collection Koha
country Argentina
countrycode AR
component Bibliográfico
access En linea
En linea
databasecode cat-ceiba
tag biblioteca
region America del Sur
libraryname Biblioteca Central FAUBA
language eng
topic BACTERIAL ENZYME
BETA GLUCOSIDASE
RAFFINASE
UNCLASSIFIED DRUG
BACILLUS
BACTERIUM
BACTERIUM ISOLATE
CONTROLLED STUDY
INSECT
NEZARA VIRIDULA
MICROCOCCUS
BACTERIAL ENZYME
BETA GLUCOSIDASE
RAFFINASE
UNCLASSIFIED DRUG
BACILLUS
BACTERIUM
BACTERIUM ISOLATE
CONTROLLED STUDY
INSECT
NEZARA VIRIDULA
MICROCOCCUS
spellingShingle BACTERIAL ENZYME
BETA GLUCOSIDASE
RAFFINASE
UNCLASSIFIED DRUG
BACILLUS
BACTERIUM
BACTERIUM ISOLATE
CONTROLLED STUDY
INSECT
NEZARA VIRIDULA
MICROCOCCUS
BACTERIAL ENZYME
BETA GLUCOSIDASE
RAFFINASE
UNCLASSIFIED DRUG
BACILLUS
BACTERIUM
BACTERIUM ISOLATE
CONTROLLED STUDY
INSECT
NEZARA VIRIDULA
MICROCOCCUS
Medina, Virginia
Sardoy, Pedro Miguel
Soria, Marcelo Abel
Vay, Carlos A.
Gutkind, Gabriel O.
Zavala, Jorge Alberto
Characterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses
description The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized midgut microbiota of stinkbugs collected from soybean crops, different secondary plant hosts or insects at diapause on Eucalyptus trees. Our study demonstrated that while more than 54% of N. viridula adults collected in the field had no detectable bacteria in the V1-V3 midgut ventricles, the guts of the rest of stinkbugs were colonized by non-transient microbiota (NTM) and transient microbiota not present in stinkbugs at diapause. While transient microbiota Bacillus sp., Micrococcus sp., Streptomyces sp., Staphylococcus sp. and others had low abundance, NTM microbiota was represented by Yokenella sp., Pantoea sp. and Enterococcus sp. isolates. We found some isolates that showed in vitro ß-glucosidase and raffinase activities plus the ability to degrade isoflavonoids and deactivate soybean protease inhibitors. Our results suggest that the stinkbugs NTM microbiota may impact on nutrition, detoxification and deactivation of chemical defenses, and Enterococcus sp., Yokenella sp. and Pantoea sp. strains might help stinkbugs to feed on soybean developing seeds in spite of its chemical defenses.
format Texto
topic_facet BACTERIAL ENZYME
BETA GLUCOSIDASE
RAFFINASE
UNCLASSIFIED DRUG
BACILLUS
BACTERIUM
BACTERIUM ISOLATE
CONTROLLED STUDY
INSECT
NEZARA VIRIDULA
MICROCOCCUS
author Medina, Virginia
Sardoy, Pedro Miguel
Soria, Marcelo Abel
Vay, Carlos A.
Gutkind, Gabriel O.
Zavala, Jorge Alberto
author_facet Medina, Virginia
Sardoy, Pedro Miguel
Soria, Marcelo Abel
Vay, Carlos A.
Gutkind, Gabriel O.
Zavala, Jorge Alberto
author_sort Medina, Virginia
title Characterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses
title_short Characterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses
title_full Characterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses
title_fullStr Characterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses
title_full_unstemmed Characterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses
title_sort characterized non - transient microbiota from stinkbug (nezara viridula) midgut deactivates soybean chemical defenses
url http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45959
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
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