Non - target - site glyphosate resistance in Echinochloa colona from Western Australia
An Echinochloa colona population from Western Australia has evolved resistance to glyphosate. This current study investigates the physiological, molecular and biochemical basis of glyphosate resistance in this population. To minimise genetic differences the susceptible (S) and resistance (R) phenotypes were isolated from within this resistant population. The S phenotype was found to accumulate significantly more shikimate in the leaf tissue than the R phenotype following glyphosate treatment. Target - site EPSPS gene sequencing revealed no resistance mutations and EPSPS gene expression was similar between the S and R phenotypes. Thus, glyphosate resistance in this population is unlikely target - site based. Similarly, there were no significant differences between the S and R phenotypes in glyphosate leaf uptake and translocation at the whole plant level. It is also unlikely that the resistance is associated with glyphosate metabolism as no major glyphosate metabolites were detected in leaf tissue of the S and R phenotypes. Despite much effort the exact glyphosate resistance mechanisms in this R population remain unclear, and novel resistance mechanisms are to be determined.
Main Authors: | , , , , , |
---|---|
Format: | Texto biblioteca |
Language: | eng |
Subjects: | ECHINOCHLOA COLONA, EPSPS, GLYPHOSATE, NON - TARGET - SITE RESISTANCE, |
Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45826 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
KOHA-OAI-AGRO:45826 |
---|---|
record_format |
koha |
spelling |
KOHA-OAI-AGRO:458262023-08-11T15:21:54Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45826http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGNon - target - site glyphosate resistance in Echinochloa colona from Western AustraliaGoh, Sou ShengYu, QinHan, HepingVila Aiub, Martín MiguelBusi, RobertoPowles, Stephen B.textengapplication/pdfAn Echinochloa colona population from Western Australia has evolved resistance to glyphosate. This current study investigates the physiological, molecular and biochemical basis of glyphosate resistance in this population. To minimise genetic differences the susceptible (S) and resistance (R) phenotypes were isolated from within this resistant population. The S phenotype was found to accumulate significantly more shikimate in the leaf tissue than the R phenotype following glyphosate treatment. Target - site EPSPS gene sequencing revealed no resistance mutations and EPSPS gene expression was similar between the S and R phenotypes. Thus, glyphosate resistance in this population is unlikely target - site based. Similarly, there were no significant differences between the S and R phenotypes in glyphosate leaf uptake and translocation at the whole plant level. It is also unlikely that the resistance is associated with glyphosate metabolism as no major glyphosate metabolites were detected in leaf tissue of the S and R phenotypes. Despite much effort the exact glyphosate resistance mechanisms in this R population remain unclear, and novel resistance mechanisms are to be determined.An Echinochloa colona population from Western Australia has evolved resistance to glyphosate. This current study investigates the physiological, molecular and biochemical basis of glyphosate resistance in this population. To minimise genetic differences the susceptible (S) and resistance (R) phenotypes were isolated from within this resistant population. The S phenotype was found to accumulate significantly more shikimate in the leaf tissue than the R phenotype following glyphosate treatment. Target - site EPSPS gene sequencing revealed no resistance mutations and EPSPS gene expression was similar between the S and R phenotypes. Thus, glyphosate resistance in this population is unlikely target - site based. Similarly, there were no significant differences between the S and R phenotypes in glyphosate leaf uptake and translocation at the whole plant level. It is also unlikely that the resistance is associated with glyphosate metabolism as no major glyphosate metabolites were detected in leaf tissue of the S and R phenotypes. Despite much effort the exact glyphosate resistance mechanisms in this R population remain unclear, and novel resistance mechanisms are to be determined.ECHINOCHLOA COLONAEPSPSGLYPHOSATENON - TARGET - SITE RESISTANCECrop Protection |
institution |
UBA FA |
collection |
Koha |
country |
Argentina |
countrycode |
AR |
component |
Bibliográfico |
access |
En linea En linea |
databasecode |
cat-ceiba |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Biblioteca Central FAUBA |
language |
eng |
topic |
ECHINOCHLOA COLONA EPSPS GLYPHOSATE NON - TARGET - SITE RESISTANCE ECHINOCHLOA COLONA EPSPS GLYPHOSATE NON - TARGET - SITE RESISTANCE |
spellingShingle |
ECHINOCHLOA COLONA EPSPS GLYPHOSATE NON - TARGET - SITE RESISTANCE ECHINOCHLOA COLONA EPSPS GLYPHOSATE NON - TARGET - SITE RESISTANCE Goh, Sou Sheng Yu, Qin Han, Heping Vila Aiub, Martín Miguel Busi, Roberto Powles, Stephen B. Non - target - site glyphosate resistance in Echinochloa colona from Western Australia |
description |
An Echinochloa colona population from Western Australia has evolved resistance to glyphosate. This current study investigates the physiological, molecular and biochemical basis of glyphosate resistance in this population. To minimise genetic differences the susceptible (S) and resistance (R) phenotypes were isolated from within this resistant population. The S phenotype was found to accumulate significantly more shikimate in the leaf tissue than the R phenotype following glyphosate treatment. Target - site EPSPS gene sequencing revealed no resistance mutations and EPSPS gene expression was similar between the S and R phenotypes. Thus, glyphosate resistance in this population is unlikely target - site based. Similarly, there were no significant differences between the S and R phenotypes in glyphosate leaf uptake and translocation at the whole plant level. It is also unlikely that the resistance is associated with glyphosate metabolism as no major glyphosate metabolites were detected in leaf tissue of the S and R phenotypes. Despite much effort the exact glyphosate resistance mechanisms in this R population remain unclear, and novel resistance mechanisms are to be determined. |
format |
Texto |
topic_facet |
ECHINOCHLOA COLONA EPSPS GLYPHOSATE NON - TARGET - SITE RESISTANCE |
author |
Goh, Sou Sheng Yu, Qin Han, Heping Vila Aiub, Martín Miguel Busi, Roberto Powles, Stephen B. |
author_facet |
Goh, Sou Sheng Yu, Qin Han, Heping Vila Aiub, Martín Miguel Busi, Roberto Powles, Stephen B. |
author_sort |
Goh, Sou Sheng |
title |
Non - target - site glyphosate resistance in Echinochloa colona from Western Australia |
title_short |
Non - target - site glyphosate resistance in Echinochloa colona from Western Australia |
title_full |
Non - target - site glyphosate resistance in Echinochloa colona from Western Australia |
title_fullStr |
Non - target - site glyphosate resistance in Echinochloa colona from Western Australia |
title_full_unstemmed |
Non - target - site glyphosate resistance in Echinochloa colona from Western Australia |
title_sort |
non - target - site glyphosate resistance in echinochloa colona from western australia |
url |
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45826 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
work_keys_str_mv |
AT gohsousheng nontargetsiteglyphosateresistanceinechinochloacolonafromwesternaustralia AT yuqin nontargetsiteglyphosateresistanceinechinochloacolonafromwesternaustralia AT hanheping nontargetsiteglyphosateresistanceinechinochloacolonafromwesternaustralia AT vilaaiubmartinmiguel nontargetsiteglyphosateresistanceinechinochloacolonafromwesternaustralia AT busiroberto nontargetsiteglyphosateresistanceinechinochloacolonafromwesternaustralia AT powlesstephenb nontargetsiteglyphosateresistanceinechinochloacolonafromwesternaustralia |
_version_ |
1774396328631074816 |