Biochemical performance of bean seedlings under water restriction at the beginning of development
The present work aimed to evaluate the performance of bean cultivars submitted to water deficit in the initial development of plants. The experiment was conducted at the Federal University of Pelotas. The seeds used were from the cultivars BRS Esteio and IPR Tuiuú, both cultivars from the black group. Water restriction was imposed using PEG-6000 as an osmotic potential reducer to perform the germination test. On the tenth day of germination, seedlings were collected for the evaluation of the following tests: levels of proline, hydrogen peroxide, lipid peroxidation and antioxidant enzymes that were evaluated through the specific activity of the enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). The design used was completely randomized, in the factorial scheme two cultivars for four osmotic potentials. The data were subjected to analysis of variance and the F values when significant were compared using the Tukey test at 5% probability. The results demonstrate that water restriction, according to the osmotic potential, affects CAT, SOD, APX enzymes in a different way. Which can result in plants less adapted to water stress.
| Main Authors: | , , , , , , , |
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| Format: | Digital revista |
| Language: | por |
| Published: |
Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata
2021
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| Online Access: | https://revistas.unlp.edu.ar/revagro/article/view/12244 |
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| Summary: | The present work aimed to evaluate the performance of bean cultivars submitted to water deficit in the initial development of plants. The experiment was conducted at the Federal University of Pelotas. The seeds used were from the cultivars BRS Esteio and IPR Tuiuú, both cultivars from the black group. Water restriction was imposed using PEG-6000 as an osmotic potential reducer to perform the germination test. On the tenth day of germination, seedlings were collected for the evaluation of the following tests: levels of proline, hydrogen peroxide, lipid peroxidation and antioxidant enzymes that were evaluated through the specific activity of the enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). The design used was completely randomized, in the factorial scheme two cultivars for four osmotic potentials. The data were subjected to analysis of variance and the F values when significant were compared using the Tukey test at 5% probability. The results demonstrate that water restriction, according to the osmotic potential, affects CAT, SOD, APX enzymes in a different way. Which can result in plants less adapted to water stress. |
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