Cell-surface translational dynamics of nicotinic acetylcholine receptors

Abstract: Synapse efficacy heavily relies on the number of neurotransmitter receptors available at a given time. In addition to the equilibrium between the biosynthetic production, exocytic delivery and recycling of receptors on the one hand, and the endocytic internalization on the other, lateral diffusion and clustering of receptors at the cell membrane play key roles in determining the amount of active receptors at the synapse. Mobile receptors traffic between reservoir compartments and the synapse by thermally driven Brownian motion, and become immobilized at the peri-synaptic region or the synapse by: (a) clustering mediated by homotropic inter-molecular receptor-receptor associations; (b) heterotropic associations with non-receptor scaffolding proteins or the subjacent cytoskeletal meshwork, leading to diffusional "trapping," and (c) protein-lipid interactions, particularly with the neutral lipid cholesterol. This review assesses the contribution of some of these mechanisms to the supramolecular organization and dynamics of the paradigm neurotransmitter receptor of muscle and neuronal cells -the nicotinic acetylcholine receptor (nAChR). Currently available information stemming from various complementary biophysical techniques commonly used to interrogate the dynamics of cell-surface components is critically discussed. The translational mobility of nAChRs at the cell surface differs between muscle and neuronal receptors in terms of diffusion coefficients and residence intervals at the synapse, which cover an ample range of time regimes. A peculiar feature of brain α7 nAChR is its ability to spend much of its time confined peri-synaptically, vicinal to glutamatergic (excitatory) and GABAergic (inhibitory) synapses. An important function of the α7 nAChR may thus be visiting the territories of other neurotransmitter receptors, differentially regulating the dynamic equilibrium between excitation and inhibition, depending on its residence time in each domain.

Saved in:
Bibliographic Details
Main Author: Barrantes, Francisco José
Format: Artículo biblioteca
Language:eng
Published: Frontiers 2014
Subjects:MEDICINA, COLESTEROL, RECEPTORES, NEUROTRANSMISORES, MEMBRANAS CELULARES,
Online Access:https://repositorio.uca.edu.ar/handle/123456789/8750
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:ucacris:123456789-8750
record_format koha
spelling oai:ucacris:123456789-87502019-09-18T04:14:18Z Cell-surface translational dynamics of nicotinic acetylcholine receptors Barrantes, Francisco José MEDICINA COLESTEROL RECEPTORES NEUROTRANSMISORES MEMBRANAS CELULARES Abstract: Synapse efficacy heavily relies on the number of neurotransmitter receptors available at a given time. In addition to the equilibrium between the biosynthetic production, exocytic delivery and recycling of receptors on the one hand, and the endocytic internalization on the other, lateral diffusion and clustering of receptors at the cell membrane play key roles in determining the amount of active receptors at the synapse. Mobile receptors traffic between reservoir compartments and the synapse by thermally driven Brownian motion, and become immobilized at the peri-synaptic region or the synapse by: (a) clustering mediated by homotropic inter-molecular receptor-receptor associations; (b) heterotropic associations with non-receptor scaffolding proteins or the subjacent cytoskeletal meshwork, leading to diffusional "trapping," and (c) protein-lipid interactions, particularly with the neutral lipid cholesterol. This review assesses the contribution of some of these mechanisms to the supramolecular organization and dynamics of the paradigm neurotransmitter receptor of muscle and neuronal cells -the nicotinic acetylcholine receptor (nAChR). Currently available information stemming from various complementary biophysical techniques commonly used to interrogate the dynamics of cell-surface components is critically discussed. The translational mobility of nAChRs at the cell surface differs between muscle and neuronal receptors in terms of diffusion coefficients and residence intervals at the synapse, which cover an ample range of time regimes. A peculiar feature of brain α7 nAChR is its ability to spend much of its time confined peri-synaptically, vicinal to glutamatergic (excitatory) and GABAergic (inhibitory) synapses. An important function of the α7 nAChR may thus be visiting the territories of other neurotransmitter receptors, differentially regulating the dynamic equilibrium between excitation and inhibition, depending on its residence time in each domain. 2019-09-17T12:33:44Z 2019-09-17T12:33:44Z 2014 Artículo Barrantes FJ (2014). Cell-surface translational dynamics of nicotinic acetyl-choline receptors. Front. Synaptic Neurosci. 6:25. doi:10.3389/fnsyn.2014.00025. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8750 1663-3563 (online) https://repositorio.uca.edu.ar/handle/123456789/8750 10.3389/fnsyn.2014.00025 eng Acceso abierto http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Frontiers Frontiers in Synaptic Neuroscience Vol. 6, N° 25, 2014
institution UCA
collection DSpace
country Argentina
countrycode AR
component Bibliográfico
access En linea
databasecode dig-uca
tag biblioteca
region America del Sur
libraryname Sistema de bibliotecas de la UCA
language eng
topic MEDICINA
COLESTEROL
RECEPTORES
NEUROTRANSMISORES
MEMBRANAS CELULARES
MEDICINA
COLESTEROL
RECEPTORES
NEUROTRANSMISORES
MEMBRANAS CELULARES
spellingShingle MEDICINA
COLESTEROL
RECEPTORES
NEUROTRANSMISORES
MEMBRANAS CELULARES
MEDICINA
COLESTEROL
RECEPTORES
NEUROTRANSMISORES
MEMBRANAS CELULARES
Barrantes, Francisco José
Cell-surface translational dynamics of nicotinic acetylcholine receptors
description Abstract: Synapse efficacy heavily relies on the number of neurotransmitter receptors available at a given time. In addition to the equilibrium between the biosynthetic production, exocytic delivery and recycling of receptors on the one hand, and the endocytic internalization on the other, lateral diffusion and clustering of receptors at the cell membrane play key roles in determining the amount of active receptors at the synapse. Mobile receptors traffic between reservoir compartments and the synapse by thermally driven Brownian motion, and become immobilized at the peri-synaptic region or the synapse by: (a) clustering mediated by homotropic inter-molecular receptor-receptor associations; (b) heterotropic associations with non-receptor scaffolding proteins or the subjacent cytoskeletal meshwork, leading to diffusional "trapping," and (c) protein-lipid interactions, particularly with the neutral lipid cholesterol. This review assesses the contribution of some of these mechanisms to the supramolecular organization and dynamics of the paradigm neurotransmitter receptor of muscle and neuronal cells -the nicotinic acetylcholine receptor (nAChR). Currently available information stemming from various complementary biophysical techniques commonly used to interrogate the dynamics of cell-surface components is critically discussed. The translational mobility of nAChRs at the cell surface differs between muscle and neuronal receptors in terms of diffusion coefficients and residence intervals at the synapse, which cover an ample range of time regimes. A peculiar feature of brain α7 nAChR is its ability to spend much of its time confined peri-synaptically, vicinal to glutamatergic (excitatory) and GABAergic (inhibitory) synapses. An important function of the α7 nAChR may thus be visiting the territories of other neurotransmitter receptors, differentially regulating the dynamic equilibrium between excitation and inhibition, depending on its residence time in each domain.
format Artículo
topic_facet MEDICINA
COLESTEROL
RECEPTORES
NEUROTRANSMISORES
MEMBRANAS CELULARES
author Barrantes, Francisco José
author_facet Barrantes, Francisco José
author_sort Barrantes, Francisco José
title Cell-surface translational dynamics of nicotinic acetylcholine receptors
title_short Cell-surface translational dynamics of nicotinic acetylcholine receptors
title_full Cell-surface translational dynamics of nicotinic acetylcholine receptors
title_fullStr Cell-surface translational dynamics of nicotinic acetylcholine receptors
title_full_unstemmed Cell-surface translational dynamics of nicotinic acetylcholine receptors
title_sort cell-surface translational dynamics of nicotinic acetylcholine receptors
publisher Frontiers
publishDate 2014
url https://repositorio.uca.edu.ar/handle/123456789/8750
work_keys_str_mv AT barrantesfranciscojose cellsurfacetranslationaldynamicsofnicotinicacetylcholinereceptors
_version_ 1756275845609029632