Fermentation of hexoses and pentoses from hydrolyzed soybean hull into ethanol and xylitol by Candida guilliermondii BL 13
Abstract This work investigated the ability of a recently isolated strain of Candida guilliermondii to convert hexoses and pentoses obtained from acid-enzymatic soybean hull hydrolysates into ethanol and, in smaller amounts, into xylitol. Operational conditions and media formulation were optimized concerning ethanol production using experimental designs (Plackett-Burman and Central Composite Design). Results showed that C. guilliermondii BL 13 was capable of growing in non-supplemented, non-detoxified biomass hydrolysates, and the best culture conditions were determined to be 28 °C, pH 5.0, and 109 CFU mL-1 of inoculum size. Ethanol productivity reached 1.4 g L-1 h-1, and maximal yields of 0.41 g g-1 were obtained, representing 80.4 % of the expected theoretical yields, whereas small amounts of xylitol were also produced. These results suggest that C. guilliermondii BL13 is a potentially useful yeast strain to be applied in second-generation ethanol production from lignocellulosic biomass based on its natural capacity to metabolize C-5 and C-6 sugars.
| Main Authors: | , , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Brazilian Society of Chemical Engineering
2017
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000400927 |
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| Summary: | Abstract This work investigated the ability of a recently isolated strain of Candida guilliermondii to convert hexoses and pentoses obtained from acid-enzymatic soybean hull hydrolysates into ethanol and, in smaller amounts, into xylitol. Operational conditions and media formulation were optimized concerning ethanol production using experimental designs (Plackett-Burman and Central Composite Design). Results showed that C. guilliermondii BL 13 was capable of growing in non-supplemented, non-detoxified biomass hydrolysates, and the best culture conditions were determined to be 28 °C, pH 5.0, and 109 CFU mL-1 of inoculum size. Ethanol productivity reached 1.4 g L-1 h-1, and maximal yields of 0.41 g g-1 were obtained, representing 80.4 % of the expected theoretical yields, whereas small amounts of xylitol were also produced. These results suggest that C. guilliermondii BL13 is a potentially useful yeast strain to be applied in second-generation ethanol production from lignocellulosic biomass based on its natural capacity to metabolize C-5 and C-6 sugars. |
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