Brazilian ground beef authentication by multiplex polymerase chain reaction

Brazilian ground beef authentication by multiplex polymerase chain reaction

ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.

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Main Authors: Oliveira,Andrey Carlos do Sacramento de, Pedroso,Silvia Cristina da Silva, Cardilli,Diogo José, Leite,Fábio Pereira Leivas, Ferreira,Gabrielle Virgínia Lopes, Silva,Andréia Silva da, Roos,Talita Bandeira, Moraes,Carina Martins de, Sousa,Roberta Sales, Monteiro,Roseli do Socorro Dias
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2018
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652
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spelling oai:scielo:S0103-847820180002006522018-03-06Brazilian ground beef authentication by multiplex polymerase chain reactionOliveira,Andrey Carlos do Sacramento dePedroso,Silvia Cristina da SilvaCardilli,Diogo JoséLeite,Fábio Pereira LeivasFerreira,Gabrielle Virgínia LopesSilva,Andréia Silva daRoos,Talita BandeiraMoraes,Carina Martins deSousa,Roberta SalesMonteiro,Roseli do Socorro Dias species identification Bos taurus Bubalusbubalis DNA adulteration ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.48 n.2 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652en10.1590/0103-8478cr20160574
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Oliveira,Andrey Carlos do Sacramento de
Pedroso,Silvia Cristina da Silva
Cardilli,Diogo José
Leite,Fábio Pereira Leivas
Ferreira,Gabrielle Virgínia Lopes
Silva,Andréia Silva da
Roos,Talita Bandeira
Moraes,Carina Martins de
Sousa,Roberta Sales
Monteiro,Roseli do Socorro Dias
spellingShingle Oliveira,Andrey Carlos do Sacramento de
Pedroso,Silvia Cristina da Silva
Cardilli,Diogo José
Leite,Fábio Pereira Leivas
Ferreira,Gabrielle Virgínia Lopes
Silva,Andréia Silva da
Roos,Talita Bandeira
Moraes,Carina Martins de
Sousa,Roberta Sales
Monteiro,Roseli do Socorro Dias
Brazilian ground beef authentication by multiplex polymerase chain reaction
author_facet Oliveira,Andrey Carlos do Sacramento de
Pedroso,Silvia Cristina da Silva
Cardilli,Diogo José
Leite,Fábio Pereira Leivas
Ferreira,Gabrielle Virgínia Lopes
Silva,Andréia Silva da
Roos,Talita Bandeira
Moraes,Carina Martins de
Sousa,Roberta Sales
Monteiro,Roseli do Socorro Dias
author_sort Oliveira,Andrey Carlos do Sacramento de
title Brazilian ground beef authentication by multiplex polymerase chain reaction
title_short Brazilian ground beef authentication by multiplex polymerase chain reaction
title_full Brazilian ground beef authentication by multiplex polymerase chain reaction
title_fullStr Brazilian ground beef authentication by multiplex polymerase chain reaction
title_full_unstemmed Brazilian ground beef authentication by multiplex polymerase chain reaction
title_sort brazilian ground beef authentication by multiplex polymerase chain reaction
description ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.
publisher Universidade Federal de Santa Maria
publishDate 2018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652
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