Restriction enzyme analysis of the chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro
The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to identified more than 20 fragments of 18 to 0.65kb. The size of Phaseolus vulgaris L. cp DNA was estimated in 140kb with the presence of a repeat sequence of about 22kb.
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| Main Authors: | , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Universidade Federal de Santa Maria
1996
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000300030 |
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| Summary: | The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to identified more than 20 fragments of 18 to 0.65kb. The size of Phaseolus vulgaris L. cp DNA was estimated in 140kb with the presence of a repeat sequence of about 22kb. |
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