Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants
This study evaluated the in vitro antibacterial activity of conventional and experimental endodontic irrigants against Enterococcus faecalis. The following substances were evaluated by direct contact test: 2.5% sodium hypochlorite (NaOCl); 2% chlorhexidine (CHX); 1% peracetic acid. After different contact periods (30 s, 1, 3, and 10 min), a neutralizing agent was applied. Serial 10-fold dilutions were prepared and plated onto tryptic soy agar (TSA) and the number of colony-forming units per milliliter (CFU/mL) was determined. Sterile saline was used as a negative control. Both 2.5% NaOCl and 2% CHX eliminated E. faecalis after 30 s of contact. Peracetic acid reduced the bacterial counts by 86% after 3 min and completely eliminated E. faecalis after 10 min. These results allow us to conclude that 1% peracetic acid is effective against E. faecalis, despite its slower action compared with 2.5% NaOCl and 2% CHX.
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Fundação Odontológica de Ribeirão Preto
2011
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oai:scielo:S0103-644020110004000042011-08-12Antibacterial effectiveness of peracetic acid and conventional endodontic irrigantsGuerreiro-Tanomaru,Juliane MariaMorgental,Renata DornellesFaria-Junior,Norberto BatistaBerbert,Fábio Luis Camargo VilelaTanomaru-filho,Mário sodium hypochlorite chlorhexidine peracetic acid root canal irrigants Endodontics This study evaluated the in vitro antibacterial activity of conventional and experimental endodontic irrigants against Enterococcus faecalis. The following substances were evaluated by direct contact test: 2.5% sodium hypochlorite (NaOCl); 2% chlorhexidine (CHX); 1% peracetic acid. After different contact periods (30 s, 1, 3, and 10 min), a neutralizing agent was applied. Serial 10-fold dilutions were prepared and plated onto tryptic soy agar (TSA) and the number of colony-forming units per milliliter (CFU/mL) was determined. Sterile saline was used as a negative control. Both 2.5% NaOCl and 2% CHX eliminated E. faecalis after 30 s of contact. Peracetic acid reduced the bacterial counts by 86% after 3 min and completely eliminated E. faecalis after 10 min. These results allow us to conclude that 1% peracetic acid is effective against E. faecalis, despite its slower action compared with 2.5% NaOCl and 2% CHX.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.22 n.4 20112011-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000400004en10.1590/S0103-64402011000400004 |
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Guerreiro-Tanomaru,Juliane Maria Morgental,Renata Dornelles Faria-Junior,Norberto Batista Berbert,Fábio Luis Camargo Vilela Tanomaru-filho,Mário |
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Guerreiro-Tanomaru,Juliane Maria Morgental,Renata Dornelles Faria-Junior,Norberto Batista Berbert,Fábio Luis Camargo Vilela Tanomaru-filho,Mário Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants |
author_facet |
Guerreiro-Tanomaru,Juliane Maria Morgental,Renata Dornelles Faria-Junior,Norberto Batista Berbert,Fábio Luis Camargo Vilela Tanomaru-filho,Mário |
author_sort |
Guerreiro-Tanomaru,Juliane Maria |
title |
Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants |
title_short |
Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants |
title_full |
Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants |
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Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants |
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Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants |
title_sort |
antibacterial effectiveness of peracetic acid and conventional endodontic irrigants |
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This study evaluated the in vitro antibacterial activity of conventional and experimental endodontic irrigants against Enterococcus faecalis. The following substances were evaluated by direct contact test: 2.5% sodium hypochlorite (NaOCl); 2% chlorhexidine (CHX); 1% peracetic acid. After different contact periods (30 s, 1, 3, and 10 min), a neutralizing agent was applied. Serial 10-fold dilutions were prepared and plated onto tryptic soy agar (TSA) and the number of colony-forming units per milliliter (CFU/mL) was determined. Sterile saline was used as a negative control. Both 2.5% NaOCl and 2% CHX eliminated E. faecalis after 30 s of contact. Peracetic acid reduced the bacterial counts by 86% after 3 min and completely eliminated E. faecalis after 10 min. These results allow us to conclude that 1% peracetic acid is effective against E. faecalis, despite its slower action compared with 2.5% NaOCl and 2% CHX. |
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Fundação Odontológica de Ribeirão Preto |
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2011 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000400004 |
work_keys_str_mv |
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