Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning
Abstract Purpose Patients with diabetes are vulnerable to myocardial I/R (ischaemia/reperfusion) injury, but are not responsive to IPO (ischaemic post-conditioning). We hypothesized that decreased cardiac Adiponectin (APN) is responsible for the loss of diabetic heart sensitivity to IPO cardioprotecton. Methods Diabetic rats were subjected to I/R injury (30 min of LAD occlusion followed by 120 min of reperfusion). Myocardial infarct area was determined by TTC staining. Cardiac function was monitored by a microcatheter. ANP, 15-F2t-isoprostane, nitrotyrosine and MDA were measured by assay kits. Levels of p-Akt, total-Akt and GAPDH were determined by Western Blot. Results Diabetic rats subjected to myocardial IR exhibited severe myocardial infarction and oxidative stress injury, lower APN in the plasma and cardiac p-Akt expression ( P <0.05). IPO significantly attenuated myocardial injury and up-regulated plasma APN content and cardiac p-Akt expression in non-diabetic rats but not in diabetic rats. Linear correlation analysis showed that the expression of adiponectin was positively correlated with p-Akt and negatively correlated with myocardial infarction area ( P <0.01). Conclusion Protective effect of IPO was tightly correlated with the expression of adiponectin, exacerbation of I/R injury and ineffectiveness of IPO was partially due to the decline of adiponectin and inactivation of Akt in diabetes mellitus.
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2020
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000100204 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0102-86502020000100204 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0102-865020200001002042020-03-18Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioningCao,ChenLiu,Hui-minLi,WeiWu,YangLeng,YanXue,RuiChen,RongTang,Ling-huaSun,QianXia,ZhongyuanTang,Qi-zhuShen,Di-feiMeng,Qing-tao Adiponectin Diabetes Reperfusion Injury Ischemic Postconditioning Abstract Purpose Patients with diabetes are vulnerable to myocardial I/R (ischaemia/reperfusion) injury, but are not responsive to IPO (ischaemic post-conditioning). We hypothesized that decreased cardiac Adiponectin (APN) is responsible for the loss of diabetic heart sensitivity to IPO cardioprotecton. Methods Diabetic rats were subjected to I/R injury (30 min of LAD occlusion followed by 120 min of reperfusion). Myocardial infarct area was determined by TTC staining. Cardiac function was monitored by a microcatheter. ANP, 15-F2t-isoprostane, nitrotyrosine and MDA were measured by assay kits. Levels of p-Akt, total-Akt and GAPDH were determined by Western Blot. Results Diabetic rats subjected to myocardial IR exhibited severe myocardial infarction and oxidative stress injury, lower APN in the plasma and cardiac p-Akt expression ( P <0.05). IPO significantly attenuated myocardial injury and up-regulated plasma APN content and cardiac p-Akt expression in non-diabetic rats but not in diabetic rats. Linear correlation analysis showed that the expression of adiponectin was positively correlated with p-Akt and negatively correlated with myocardial infarction area ( P <0.01). Conclusion Protective effect of IPO was tightly correlated with the expression of adiponectin, exacerbation of I/R injury and ineffectiveness of IPO was partially due to the decline of adiponectin and inactivation of Akt in diabetes mellitus.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.35 n.1 20202020-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000100204en10.1590/s0102-865020200010000007 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Cao,Chen Liu,Hui-min Li,Wei Wu,Yang Leng,Yan Xue,Rui Chen,Rong Tang,Ling-hua Sun,Qian Xia,Zhongyuan Tang,Qi-zhu Shen,Di-fei Meng,Qing-tao |
spellingShingle |
Cao,Chen Liu,Hui-min Li,Wei Wu,Yang Leng,Yan Xue,Rui Chen,Rong Tang,Ling-hua Sun,Qian Xia,Zhongyuan Tang,Qi-zhu Shen,Di-fei Meng,Qing-tao Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning |
author_facet |
Cao,Chen Liu,Hui-min Li,Wei Wu,Yang Leng,Yan Xue,Rui Chen,Rong Tang,Ling-hua Sun,Qian Xia,Zhongyuan Tang,Qi-zhu Shen,Di-fei Meng,Qing-tao |
author_sort |
Cao,Chen |
title |
Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning |
title_short |
Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning |
title_full |
Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning |
title_fullStr |
Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning |
title_full_unstemmed |
Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning |
title_sort |
role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning |
description |
Abstract Purpose Patients with diabetes are vulnerable to myocardial I/R (ischaemia/reperfusion) injury, but are not responsive to IPO (ischaemic post-conditioning). We hypothesized that decreased cardiac Adiponectin (APN) is responsible for the loss of diabetic heart sensitivity to IPO cardioprotecton. Methods Diabetic rats were subjected to I/R injury (30 min of LAD occlusion followed by 120 min of reperfusion). Myocardial infarct area was determined by TTC staining. Cardiac function was monitored by a microcatheter. ANP, 15-F2t-isoprostane, nitrotyrosine and MDA were measured by assay kits. Levels of p-Akt, total-Akt and GAPDH were determined by Western Blot. Results Diabetic rats subjected to myocardial IR exhibited severe myocardial infarction and oxidative stress injury, lower APN in the plasma and cardiac p-Akt expression ( P <0.05). IPO significantly attenuated myocardial injury and up-regulated plasma APN content and cardiac p-Akt expression in non-diabetic rats but not in diabetic rats. Linear correlation analysis showed that the expression of adiponectin was positively correlated with p-Akt and negatively correlated with myocardial infarction area ( P <0.01). Conclusion Protective effect of IPO was tightly correlated with the expression of adiponectin, exacerbation of I/R injury and ineffectiveness of IPO was partially due to the decline of adiponectin and inactivation of Akt in diabetes mellitus. |
publisher |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publishDate |
2020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000100204 |
work_keys_str_mv |
AT caochen roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT liuhuimin roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT liwei roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT wuyang roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT lengyan roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT xuerui roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT chenrong roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT tanglinghua roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT sunqian roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT xiazhongyuan roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT tangqizhu roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT shendifei roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning AT mengqingtao roleofadiponectinindiabetesmyocardialischemiareperfusioninjuryandischemicpostconditioning |
_version_ |
1756401047743496192 |