Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types
Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for the accurate detection of the distinct mating types of H. capsulatum. Among the 26 isolates tested, 20 had MAT1-1 genotype, while six showed MAT1-2 genotype, in agreement with the uniplex PCR results. These results suggest that multiplex PCR is a fast and specific tool for screening H. capsulatum mating types.
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Main Authors: | Almeida-Silva,Fernando, Barbedo,Leonardo Silva, Taylor,Maria Lucia, Muniz,Mauro de Medeiros, Guimarães,Allan Jefferson, Zancopé-Oliveira,Rosely Maria |
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Format: | Digital revista |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2018
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018001000402 |
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