A one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herd
Bovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July to 15.25% in November. The confinement within pens resulted in prevalence of 11%. Most animals (61.86%; 75/118) shed E. coli O157 at least in one sampling occasion. Of the positive animals, 82.19%, 16.44%, and 1.37% were stx positive on one, two and three sampling occasions, respectively. All the E. coli O157 isolated strains carried the genes eae and rfbO157, whereas 11%, 33% and 56% contained stx1, stx2 and stx1/stx2, respectively. The stx1/stx2 and stx2 types were significantly higher during the grazing and finishing periods, respectively, in comparison with the rearing and grazing periods. The presence of stx2a subtype was evident in four isolates, whereas stx2c was present in at least seven. However, both subtypes were present simultaneously in two isolates. The stx1/stx2c, stx1/stx2d and stx1/stx2NT genotypes occurred in 24, 2 and 15 isolates, respectively. The simultaneous occurrence of stx1 and stx2c significantly increased during grazing. Some cases of within-pen and between-pen transmission occurred throughout the study. Contagion levels during in-field grazing were higher than during permanent confinement in the pens. Thus, the individual patterns of shedding varied depending on the proportion of animals shedding the bacteria within pens and the time of shedding.
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Elsevier
2019-12-01
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Subjects: | Escherichia coli, Vacas de cría, Virulencia, Genética, Suckler Cows, Genetics, Beef Cattle, Virulence, Ganado de Carne, |
Online Access: | http://hdl.handle.net/20.500.12123/6345 https://www.sciencedirect.com/science/article/pii/S0034528819303959 https://doi.org/10.1016/j.rvsc.2019.10.001 |
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Escherichia coli Vacas de cría Virulencia Genética Suckler Cows Genetics Beef Cattle Virulence Ganado de Carne Escherichia coli Vacas de cría Virulencia Genética Suckler Cows Genetics Beef Cattle Virulence Ganado de Carne |
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Escherichia coli Vacas de cría Virulencia Genética Suckler Cows Genetics Beef Cattle Virulence Ganado de Carne Escherichia coli Vacas de cría Virulencia Genética Suckler Cows Genetics Beef Cattle Virulence Ganado de Carne Rhades, Luis Larzabal, Mariano Bentancor, Adriana Sabio Y Garcia, Julia Veronica Babinec, Francisco Jose Cataldi, Angel Adrian Amigo, Natalia Baldone, Valeria Natalia Urquiza, Lucas Federico Delicia, Pablo Javier Fort, Marcelo Cristian A one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herd |
description |
Bovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July to 15.25% in November. The confinement within pens resulted in prevalence
of 11%. Most animals (61.86%; 75/118) shed E. coli O157 at least in one sampling occasion. Of the
positive animals, 82.19%, 16.44%, and 1.37% were stx positive on one, two and three sampling occasions,
respectively. All the E. coli O157 isolated strains carried the genes eae and rfbO157, whereas 11%, 33% and 56% contained stx1, stx2 and stx1/stx2, respectively. The stx1/stx2 and stx2 types were significantly higher during the grazing and finishing periods, respectively, in comparison with the rearing and grazing periods. The presence of stx2a subtype was evident in four isolates, whereas stx2c was present in at least seven. However, both subtypes
were present simultaneously in two isolates. The stx1/stx2c, stx1/stx2d and stx1/stx2NT genotypes occurred in 24, 2 and 15 isolates, respectively. The simultaneous occurrence of stx1 and stx2c significantly increased during grazing. Some cases of within-pen and between-pen transmission occurred throughout the study. Contagion levels during in-field grazing were higher than during permanent confinement in the pens. Thus, the individual
patterns of shedding varied depending on the proportion of animals shedding the bacteria within pens and the time of shedding. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Escherichia coli Vacas de cría Virulencia Genética Suckler Cows Genetics Beef Cattle Virulence Ganado de Carne |
author |
Rhades, Luis Larzabal, Mariano Bentancor, Adriana Sabio Y Garcia, Julia Veronica Babinec, Francisco Jose Cataldi, Angel Adrian Amigo, Natalia Baldone, Valeria Natalia Urquiza, Lucas Federico Delicia, Pablo Javier Fort, Marcelo Cristian |
author_facet |
Rhades, Luis Larzabal, Mariano Bentancor, Adriana Sabio Y Garcia, Julia Veronica Babinec, Francisco Jose Cataldi, Angel Adrian Amigo, Natalia Baldone, Valeria Natalia Urquiza, Lucas Federico Delicia, Pablo Javier Fort, Marcelo Cristian |
author_sort |
Rhades, Luis |
title |
A one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herd |
title_short |
A one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herd |
title_full |
A one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herd |
title_fullStr |
A one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herd |
title_full_unstemmed |
A one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herd |
title_sort |
one-year longitudinal study of enterohemorrhagic escherichia coli o157 fecal shedding in a beef cattle herd |
publisher |
Elsevier |
publishDate |
2019-12-01 |
url |
http://hdl.handle.net/20.500.12123/6345 https://www.sciencedirect.com/science/article/pii/S0034528819303959 https://doi.org/10.1016/j.rvsc.2019.10.001 |
work_keys_str_mv |
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oai:localhost:20.500.12123-63452019-11-20T13:58:37Z A one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herd Rhades, Luis Larzabal, Mariano Bentancor, Adriana Sabio Y Garcia, Julia Veronica Babinec, Francisco Jose Cataldi, Angel Adrian Amigo, Natalia Baldone, Valeria Natalia Urquiza, Lucas Federico Delicia, Pablo Javier Fort, Marcelo Cristian Escherichia coli Vacas de cría Virulencia Genética Suckler Cows Genetics Beef Cattle Virulence Ganado de Carne Bovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July to 15.25% in November. The confinement within pens resulted in prevalence of 11%. Most animals (61.86%; 75/118) shed E. coli O157 at least in one sampling occasion. Of the positive animals, 82.19%, 16.44%, and 1.37% were stx positive on one, two and three sampling occasions, respectively. All the E. coli O157 isolated strains carried the genes eae and rfbO157, whereas 11%, 33% and 56% contained stx1, stx2 and stx1/stx2, respectively. The stx1/stx2 and stx2 types were significantly higher during the grazing and finishing periods, respectively, in comparison with the rearing and grazing periods. The presence of stx2a subtype was evident in four isolates, whereas stx2c was present in at least seven. However, both subtypes were present simultaneously in two isolates. The stx1/stx2c, stx1/stx2d and stx1/stx2NT genotypes occurred in 24, 2 and 15 isolates, respectively. The simultaneous occurrence of stx1 and stx2c significantly increased during grazing. Some cases of within-pen and between-pen transmission occurred throughout the study. Contagion levels during in-field grazing were higher than during permanent confinement in the pens. Thus, the individual patterns of shedding varied depending on the proportion of animals shedding the bacteria within pens and the time of shedding. Estación Experimental Agropecuaria Anguil Fil: Rhades, Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Bentancor, Adriana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Larzabal, Mariano.Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sabio Y Garcia, Julia Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Babinec, Francisco.Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Baldone, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Urquiza, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Delicia, Pablo Javier. Universidad Tecnológica Nacional. Facultad Regional Rosario; Argentina Fil: Fort, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina 2019-11-20T13:37:50Z 2019-11-20T13:37:50Z 2019-12-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6345 https://www.sciencedirect.com/science/article/pii/S0034528819303959 0034-5288 https://doi.org/10.1016/j.rvsc.2019.10.001 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Research in Veterinary Science 127 : 27-32 (December 2019) |