Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina
Red stripe is a bacterial disease of sugarcane causing important economic losses in Argentina that affects 30 % of the milling stems and consequently the juice quality. In this study, sugarcane leaves exhibiting red stripe symptoms were sampled in the 2008–09 growing season from 13 different sugarcane producing areas of Tucumán and Salta (northwest of Argentina). To achieve the identification and characterization of the causal agent of red stripe, bacterial isolation was performed. Species-specific PCR using Oaf1/Oar1 primers allowed the amplification of a fragment of 550 bp from approximately 50 % of the isolates; 16S rDNA sequences analysis displayed a similarity greater than 99 % with Acidovorax avenae subsp. avenae. By means of RAPD-PCR the presence of at least four different biotypes among the analyzed isolates was detected. Results of pathogenicity test allowed us to confirm A. avenae subsp. avenae as the pathogenic agent for red stripe. This study constitutes the first report on the identification and molecular characterization of this plant pathogen from the Argentina sugarcane production areas. The genetic diversity observed among A. avenae is an important factor to be considered to improve an accurate diagnosis and/or the selection of sugarcane tolerant clones.
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2013-11
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Subjects: | Caña de Azúcar, Enfermedades de las Plantas, Variación Genética, Aislamiento, PCR, Sugarcane, Plant Diseases, Genetic Variation, Isolation, Acidovorax avenae, Región Noroeste, Argentina, |
Online Access: | https://link.springer.com/article/10.1007/s10658-013-0263-y http://hdl.handle.net/20.500.12123/3102 https://doi.org/10.1007/s10658-013-0263-y |
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oai:localhost:20.500.12123-31022019-09-12T11:17:51Z Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina Fontana, Paola Daniela Rago, Alejandro Mario Fontana, Cecilia Alejandra Vignolo, Graciela Margarita Cocconcelli, Pier Sandro Mariotti Martinez, Jorge Alberto Caña de Azúcar Enfermedades de las Plantas Variación Genética Aislamiento PCR Sugarcane Plant Diseases Genetic Variation Isolation Acidovorax avenae Región Noroeste, Argentina Red stripe is a bacterial disease of sugarcane causing important economic losses in Argentina that affects 30 % of the milling stems and consequently the juice quality. In this study, sugarcane leaves exhibiting red stripe symptoms were sampled in the 2008–09 growing season from 13 different sugarcane producing areas of Tucumán and Salta (northwest of Argentina). To achieve the identification and characterization of the causal agent of red stripe, bacterial isolation was performed. Species-specific PCR using Oaf1/Oar1 primers allowed the amplification of a fragment of 550 bp from approximately 50 % of the isolates; 16S rDNA sequences analysis displayed a similarity greater than 99 % with Acidovorax avenae subsp. avenae. By means of RAPD-PCR the presence of at least four different biotypes among the analyzed isolates was detected. Results of pathogenicity test allowed us to confirm A. avenae subsp. avenae as the pathogenic agent for red stripe. This study constitutes the first report on the identification and molecular characterization of this plant pathogen from the Argentina sugarcane production areas. The genetic diversity observed among A. avenae is an important factor to be considered to improve an accurate diagnosis and/or the selection of sugarcane tolerant clones. EEA Famaillá Fil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Rago, Alejandro Mario Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fontana, Cecilia Alejandra. Universita Cattolica del Sacro Cuore. Istituto di Microbiologia-Centro di Ricerche Biotecnologiche (CRB); Italia Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos; Argentina Fil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Istituto di Microbiologia-Centro di Ricerche Biotecnologiche (CRB); Italia Fil: Mariotti Martinez, Jorge Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina 2018-08-16T13:47:40Z 2018-08-16T13:47:40Z 2013-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s10658-013-0263-y http://hdl.handle.net/20.500.12123/3102 0929-1873 1573-8469 https://doi.org/10.1007/s10658-013-0263-y eng info:eu-repo/semantics/restrictedAccess application/pdf European Journal of Plant Pathology 137 (3) : 525–534 (November 2013) |
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Caña de Azúcar Enfermedades de las Plantas Variación Genética Aislamiento PCR Sugarcane Plant Diseases Genetic Variation Isolation Acidovorax avenae Región Noroeste, Argentina Caña de Azúcar Enfermedades de las Plantas Variación Genética Aislamiento PCR Sugarcane Plant Diseases Genetic Variation Isolation Acidovorax avenae Región Noroeste, Argentina |
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Caña de Azúcar Enfermedades de las Plantas Variación Genética Aislamiento PCR Sugarcane Plant Diseases Genetic Variation Isolation Acidovorax avenae Región Noroeste, Argentina Caña de Azúcar Enfermedades de las Plantas Variación Genética Aislamiento PCR Sugarcane Plant Diseases Genetic Variation Isolation Acidovorax avenae Región Noroeste, Argentina Fontana, Paola Daniela Rago, Alejandro Mario Fontana, Cecilia Alejandra Vignolo, Graciela Margarita Cocconcelli, Pier Sandro Mariotti Martinez, Jorge Alberto Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina |
description |
Red stripe is a bacterial disease of sugarcane causing important economic losses in Argentina that affects 30 % of the milling stems and consequently the juice quality. In this study, sugarcane leaves exhibiting red stripe symptoms were sampled in the 2008–09 growing season from 13 different sugarcane producing areas of Tucumán and Salta (northwest of Argentina). To achieve the identification and characterization of the causal agent of red stripe, bacterial isolation was performed. Species-specific PCR using Oaf1/Oar1 primers allowed the amplification of a fragment of 550 bp from approximately 50 % of the isolates; 16S rDNA sequences analysis displayed a similarity greater than 99 % with Acidovorax avenae subsp. avenae. By means of RAPD-PCR the presence of at least four different biotypes among the analyzed isolates was detected. Results of pathogenicity test allowed us to confirm A. avenae subsp. avenae as the pathogenic agent for red stripe. This study constitutes the first report on the identification and molecular characterization of this plant pathogen from the Argentina sugarcane production areas. The genetic diversity observed among A. avenae is an important factor to be considered to improve an accurate diagnosis and/or the selection of sugarcane tolerant clones. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Caña de Azúcar Enfermedades de las Plantas Variación Genética Aislamiento PCR Sugarcane Plant Diseases Genetic Variation Isolation Acidovorax avenae Región Noroeste, Argentina |
author |
Fontana, Paola Daniela Rago, Alejandro Mario Fontana, Cecilia Alejandra Vignolo, Graciela Margarita Cocconcelli, Pier Sandro Mariotti Martinez, Jorge Alberto |
author_facet |
Fontana, Paola Daniela Rago, Alejandro Mario Fontana, Cecilia Alejandra Vignolo, Graciela Margarita Cocconcelli, Pier Sandro Mariotti Martinez, Jorge Alberto |
author_sort |
Fontana, Paola Daniela |
title |
Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina |
title_short |
Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina |
title_full |
Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina |
title_fullStr |
Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina |
title_full_unstemmed |
Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina |
title_sort |
isolation and genetic characterization of acidovorax avenae from red stripe infected sugarcane in northwestern argentina |
publishDate |
2013-11 |
url |
https://link.springer.com/article/10.1007/s10658-013-0263-y http://hdl.handle.net/20.500.12123/3102 https://doi.org/10.1007/s10658-013-0263-y |
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