Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
The ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce dsRNA is the use of bacteria capable of achieving high levels of in vivo synthesis. In the present study, dsRNA synthetized in vivo was used to induce gene silencing in V. destructor and evaluate their effect on the survival of both honey bees and the parasitic Varroa mites. The results evidenced that dsRNA fed to the bees engendered gene silencing in mites, inhibiting expression levels of target genes by 50%. Indeed, a reduction of 50% in Varroa survival was observed when bacterially expressed dsRNAs were administered to mite-parasitized bees. Worker bees that were fed with Varroa-targeted dsRNA by oral route showed no survival differences compared to control bees, fed with sucrose or dsRNA-GFP solutions. Our results demonstrated that specific dsRNA over-expressed in bacteria is capable of reducing mite survival by bee-mediated oral administration. This study provides an efficient and low-cost method for dsRNA production to control parasites and honey bee diseases.
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Format: | info:ar-repo/semantics/artículo biblioteca |
Language: | eng |
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Taylor and Francis
2022-02
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Subjects: | Varroa destructor, Abeja Melífera, Silenciamiento Genético, Experimentación in Vivo, Síntesis, Apis mellifera, Honey Bees, Gene Silencing, In Vivo Experimentation, dsRNA viruses, Synthesis, |
Online Access: | http://hdl.handle.net/20.500.12123/11611 https://www.tandfonline.com/doi/abs/10.1080/00218839.2022.2028967 https://doi.org/10.1080/00218839.2022.2028967 |
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Varroa destructor Abeja Melífera Silenciamiento Genético Experimentación in Vivo Síntesis Apis mellifera Honey Bees Gene Silencing In Vivo Experimentation dsRNA viruses Synthesis Varroa destructor Abeja Melífera Silenciamiento Genético Experimentación in Vivo Síntesis Apis mellifera Honey Bees Gene Silencing In Vivo Experimentation dsRNA viruses Synthesis |
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Varroa destructor Abeja Melífera Silenciamiento Genético Experimentación in Vivo Síntesis Apis mellifera Honey Bees Gene Silencing In Vivo Experimentation dsRNA viruses Synthesis Varroa destructor Abeja Melífera Silenciamiento Genético Experimentación in Vivo Síntesis Apis mellifera Honey Bees Gene Silencing In Vivo Experimentation dsRNA viruses Synthesis Muntaabski, Irina Scannapieco, Alejandra Carla Liendo, María Clara Niz, José María Russo, Romina Maria Salvador, Ricardo Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration |
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The ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce dsRNA is the use of bacteria capable of achieving high levels of in vivo synthesis. In the present study, dsRNA synthetized in vivo was used to induce gene silencing in V. destructor and evaluate their effect on the survival of both honey bees and the parasitic Varroa mites. The results evidenced that dsRNA fed to the bees engendered gene silencing in mites, inhibiting expression levels of target genes by 50%. Indeed, a reduction of 50% in Varroa survival was observed when bacterially expressed dsRNAs were administered to mite-parasitized bees. Worker bees that were fed with Varroa-targeted dsRNA by oral route showed no survival differences compared to control bees, fed with sucrose or dsRNA-GFP solutions. Our results demonstrated that specific dsRNA over-expressed in bacteria is capable of reducing mite survival by bee-mediated oral administration. This study provides an efficient and low-cost method for dsRNA production to control parasites and honey bee diseases. |
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Varroa destructor Abeja Melífera Silenciamiento Genético Experimentación in Vivo Síntesis Apis mellifera Honey Bees Gene Silencing In Vivo Experimentation dsRNA viruses Synthesis |
author |
Muntaabski, Irina Scannapieco, Alejandra Carla Liendo, María Clara Niz, José María Russo, Romina Maria Salvador, Ricardo |
author_facet |
Muntaabski, Irina Scannapieco, Alejandra Carla Liendo, María Clara Niz, José María Russo, Romina Maria Salvador, Ricardo |
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Muntaabski, Irina |
title |
Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration |
title_short |
Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration |
title_full |
Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration |
title_fullStr |
Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration |
title_full_unstemmed |
Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration |
title_sort |
bacterially expressed dsrna induces varroa destructor gene knockdown by honey bee-mediated oral administration |
publisher |
Taylor and Francis |
publishDate |
2022-02 |
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http://hdl.handle.net/20.500.12123/11611 https://www.tandfonline.com/doi/abs/10.1080/00218839.2022.2028967 https://doi.org/10.1080/00218839.2022.2028967 |
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oai:localhost:20.500.12123-116112022-04-08T17:53:25Z Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration Muntaabski, Irina Scannapieco, Alejandra Carla Liendo, María Clara Niz, José María Russo, Romina Maria Salvador, Ricardo Varroa destructor Abeja Melífera Silenciamiento Genético Experimentación in Vivo Síntesis Apis mellifera Honey Bees Gene Silencing In Vivo Experimentation dsRNA viruses Synthesis The ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce dsRNA is the use of bacteria capable of achieving high levels of in vivo synthesis. In the present study, dsRNA synthetized in vivo was used to induce gene silencing in V. destructor and evaluate their effect on the survival of both honey bees and the parasitic Varroa mites. The results evidenced that dsRNA fed to the bees engendered gene silencing in mites, inhibiting expression levels of target genes by 50%. Indeed, a reduction of 50% in Varroa survival was observed when bacterially expressed dsRNAs were administered to mite-parasitized bees. Worker bees that were fed with Varroa-targeted dsRNA by oral route showed no survival differences compared to control bees, fed with sucrose or dsRNA-GFP solutions. Our results demonstrated that specific dsRNA over-expressed in bacteria is capable of reducing mite survival by bee-mediated oral administration. This study provides an efficient and low-cost method for dsRNA production to control parasites and honey bee diseases. Instituto de Genética Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular); Argentina Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina 2022-04-08T17:46:49Z 2022-04-08T17:46:49Z 2022-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11611 https://www.tandfonline.com/doi/abs/10.1080/00218839.2022.2028967 0021-8839 https://doi.org/10.1080/00218839.2022.2028967 eng info:eu-repograntAgreement/INTA/PNAPI-1112042/AR./Estrategias multidisciplinarias para mitigar el efecto del nuevo contexto ambiental y productivo sobre la colmena. info:eu-repograntAgreement/INTA/2019-PE-E1-I017-001/2019-PE-E1-I017-001/AR./DESARROLLO DEL SECTOR APÍCOLA ORGANIZADO, SUSTENTABLE Y COMPETITIVO info:eu-repo/semantics/restrictedAccess application/pdf Taylor and Francis Journal of Apicultural Research (Published online: 01 Feb 2022) |