Cloning and Biochemical Characterization of ToFZY, a Tomato Gene Encoding a Flavin Monooxygenase Involved in a Tryptophan-dependent Auxin Biosynthesis Pathway
Indole-3-acetic acid (IAA), the main endogenous auxin, has been known for decades to be a key regulator for plant growth and development. Multiple routes have been proposed for IAA biosynthesis but physiologic roles or relevance of the different routes are still unclear. Recently, four members of the Arabidopsis thaliana YUC gene family have been implicated in an additional requirement of IAA involved in floral organ and vascular tissue formation. The loss-of-function yuc1yuc4 double mutants in Arabidopsis displayed phenotypes similar to the previously described loss-of-function floozy mutants in petunia (fzy). Moreover, it has been demonstrated that YUC1 encodes a flavin monooxygenase (FMO) that catalyzes a rate-limiting step of a tryptophan-dependent auxin biosynthesis pathway: the conversion of tryptamine to N-hydroxyl-tryptamine. Here we report on the genetic study of ToFZY, the putative tomato ortholog of YUC4 and FZY, including gene and cDNA sequence comparison and a preliminary expression analysis. In addition, we describe a novel conserved amino acid motif that may be considered a hallmark potentially useful for the identification of new YUC-like FMOs. We also demonstrate that ToFZY encodes a protein with the same enzymatic activity as YUC1. Finally, we provide evidence suggesting that the ToFZY gene belongs to a multigenic family whose members may exhibit a temporal and spatial specialization similar to that described in A. thaliana.
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Language: | English |
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Springer
2007-10-20
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Subjects: | Tomato, Flavin monooxygenase-like proteins, Tryptophan-dependent auxin biosynthesis, Tryptamine pathway, |
Online Access: | http://hdl.handle.net/10261/12974 http://dx.doi.org/10.13039/501100003339 |
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dig-ipna-es-10261-129742019-11-19T09:12:10Z Cloning and Biochemical Characterization of ToFZY, a Tomato Gene Encoding a Flavin Monooxygenase Involved in a Tryptophan-dependent Auxin Biosynthesis Pathway Expósito Rodríguez, Marino Borges, Andrés A. Borges-Pérez, Andrés Hernández, Mercedes Pérez Méndez, J. A. Invescan Consultores Consejo Superior de Investigaciones Científicas (España) Tomato Flavin monooxygenase-like proteins Tryptophan-dependent auxin biosynthesis Tryptamine pathway Indole-3-acetic acid (IAA), the main endogenous auxin, has been known for decades to be a key regulator for plant growth and development. Multiple routes have been proposed for IAA biosynthesis but physiologic roles or relevance of the different routes are still unclear. Recently, four members of the Arabidopsis thaliana YUC gene family have been implicated in an additional requirement of IAA involved in floral organ and vascular tissue formation. The loss-of-function yuc1yuc4 double mutants in Arabidopsis displayed phenotypes similar to the previously described loss-of-function floozy mutants in petunia (fzy). Moreover, it has been demonstrated that YUC1 encodes a flavin monooxygenase (FMO) that catalyzes a rate-limiting step of a tryptophan-dependent auxin biosynthesis pathway: the conversion of tryptamine to N-hydroxyl-tryptamine. Here we report on the genetic study of ToFZY, the putative tomato ortholog of YUC4 and FZY, including gene and cDNA sequence comparison and a preliminary expression analysis. In addition, we describe a novel conserved amino acid motif that may be considered a hallmark potentially useful for the identification of new YUC-like FMOs. We also demonstrate that ToFZY encodes a protein with the same enzymatic activity as YUC1. Finally, we provide evidence suggesting that the ToFZY gene belongs to a multigenic family whose members may exhibit a temporal and spatial specialization similar to that described in A. thaliana. This work was funded by an INVESCAN, S.L. grant (No. 402.01) to the CSIC and supported by a fellowship to MER, a kind gift from the same firm. 2009-05-12T06:14:58Z 2009-05-12T06:14:58Z 2007-10-20 artículo http://purl.org/coar/resource_type/c_6501 Journal of Plant Growth Regulation 26(4): 329-340 (2007) 0721-7595 http://hdl.handle.net/10261/12974 10.1007/s00344-007-9019-2 1435-8107 http://dx.doi.org/10.13039/501100003339 en http://dx.doi.org/10.1007/s00344-007-9019-2 none 2373 bytes 407285 bytes text/plain application/pdf Springer |
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Tomato Flavin monooxygenase-like proteins Tryptophan-dependent auxin biosynthesis Tryptamine pathway Tomato Flavin monooxygenase-like proteins Tryptophan-dependent auxin biosynthesis Tryptamine pathway |
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Tomato Flavin monooxygenase-like proteins Tryptophan-dependent auxin biosynthesis Tryptamine pathway Tomato Flavin monooxygenase-like proteins Tryptophan-dependent auxin biosynthesis Tryptamine pathway Expósito Rodríguez, Marino Borges, Andrés A. Borges-Pérez, Andrés Hernández, Mercedes Pérez Méndez, J. A. Cloning and Biochemical Characterization of ToFZY, a Tomato Gene Encoding a Flavin Monooxygenase Involved in a Tryptophan-dependent Auxin Biosynthesis Pathway |
description |
Indole-3-acetic acid (IAA), the main endogenous auxin, has been known for decades to be a key regulator for plant growth and development. Multiple routes have been proposed for IAA biosynthesis but physiologic roles or relevance of the different routes are still unclear. Recently, four members of the Arabidopsis thaliana YUC gene family have been implicated in an additional requirement of IAA involved in floral organ and vascular tissue formation. The loss-of-function yuc1yuc4 double mutants in Arabidopsis displayed phenotypes similar to the previously described loss-of-function floozy mutants in petunia (fzy). Moreover, it has been demonstrated that YUC1 encodes a flavin monooxygenase (FMO) that catalyzes a rate-limiting step of a tryptophan-dependent auxin biosynthesis pathway: the conversion of tryptamine to N-hydroxyl-tryptamine. Here we report on the genetic study of ToFZY, the putative tomato ortholog of YUC4 and FZY, including gene and cDNA sequence comparison and a preliminary expression analysis. In addition, we describe a novel conserved amino acid motif that may be considered a hallmark potentially useful for the identification of new YUC-like FMOs. We also demonstrate that ToFZY encodes a protein with the same enzymatic activity as YUC1. Finally, we provide evidence suggesting that the ToFZY gene belongs to a multigenic family whose members may exhibit a temporal and spatial specialization similar to that described in A. thaliana. |
author2 |
Invescan Consultores |
author_facet |
Invescan Consultores Expósito Rodríguez, Marino Borges, Andrés A. Borges-Pérez, Andrés Hernández, Mercedes Pérez Méndez, J. A. |
format |
artículo |
topic_facet |
Tomato Flavin monooxygenase-like proteins Tryptophan-dependent auxin biosynthesis Tryptamine pathway |
author |
Expósito Rodríguez, Marino Borges, Andrés A. Borges-Pérez, Andrés Hernández, Mercedes Pérez Méndez, J. A. |
author_sort |
Expósito Rodríguez, Marino |
title |
Cloning and Biochemical Characterization of ToFZY, a Tomato Gene Encoding a Flavin Monooxygenase Involved in a Tryptophan-dependent Auxin Biosynthesis Pathway |
title_short |
Cloning and Biochemical Characterization of ToFZY, a Tomato Gene Encoding a Flavin Monooxygenase Involved in a Tryptophan-dependent Auxin Biosynthesis Pathway |
title_full |
Cloning and Biochemical Characterization of ToFZY, a Tomato Gene Encoding a Flavin Monooxygenase Involved in a Tryptophan-dependent Auxin Biosynthesis Pathway |
title_fullStr |
Cloning and Biochemical Characterization of ToFZY, a Tomato Gene Encoding a Flavin Monooxygenase Involved in a Tryptophan-dependent Auxin Biosynthesis Pathway |
title_full_unstemmed |
Cloning and Biochemical Characterization of ToFZY, a Tomato Gene Encoding a Flavin Monooxygenase Involved in a Tryptophan-dependent Auxin Biosynthesis Pathway |
title_sort |
cloning and biochemical characterization of tofzy, a tomato gene encoding a flavin monooxygenase involved in a tryptophan-dependent auxin biosynthesis pathway |
publisher |
Springer |
publishDate |
2007-10-20 |
url |
http://hdl.handle.net/10261/12974 http://dx.doi.org/10.13039/501100003339 |
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