Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease

Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease

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In this study, West Nile virus (WNV) envelope (rE) protein and its domain III (rDIII) were efficiently expressed in a cost-effective system based on insect larvae as non-fermentative living biofactories. Mice immunized with the partially purified rE or rDIII elicited high antibodies titers that neutralized viral infectivity in cell culture and in suckling mice. All vaccinated animals were fully protected when challenged with neurovirulent WNV NY99. Passive transfer of protective antibodies from immunized mothers to their offspring occurred both by transplacental and lactation routes. These results indicate that the insect-derived antigens tested may constitute potential vaccine candidates to be further evaluated. © 2010 Elsevier Ltd.

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Main Authors: Alonso-Padilla, J., Jiménez de Oya, N., Blázquez, A. B., Escribano-Romero, E., Escribano, J. M., Saiz, J. C.
Format: journal article biblioteca
Language:eng
Published: 2011
Online Access:http://hdl.handle.net/20.500.12792/6037
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spelling dig-inia-es-20.500.12792-60372020-12-15T09:54:21Z Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease Alonso-Padilla, J. Jiménez de Oya, N. Blázquez, A. B. Escribano-Romero, E. Escribano, J. M. Saiz, J. C. In this study, West Nile virus (WNV) envelope (rE) protein and its domain III (rDIII) were efficiently expressed in a cost-effective system based on insect larvae as non-fermentative living biofactories. Mice immunized with the partially purified rE or rDIII elicited high antibodies titers that neutralized viral infectivity in cell culture and in suckling mice. All vaccinated animals were fully protected when challenged with neurovirulent WNV NY99. Passive transfer of protective antibodies from immunized mothers to their offspring occurred both by transplacental and lactation routes. These results indicate that the insect-derived antigens tested may constitute potential vaccine candidates to be further evaluated. © 2010 Elsevier Ltd. 2020-10-22T22:10:13Z 2020-10-22T22:10:13Z 2011 journal article http://hdl.handle.net/20.500.12792/6037 10.1016/j.vaccine.2010.12.081 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access
institution INIA ES
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country España
countrycode ES
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region Europa del Sur
libraryname Biblioteca del INIA España
language eng
description In this study, West Nile virus (WNV) envelope (rE) protein and its domain III (rDIII) were efficiently expressed in a cost-effective system based on insect larvae as non-fermentative living biofactories. Mice immunized with the partially purified rE or rDIII elicited high antibodies titers that neutralized viral infectivity in cell culture and in suckling mice. All vaccinated animals were fully protected when challenged with neurovirulent WNV NY99. Passive transfer of protective antibodies from immunized mothers to their offspring occurred both by transplacental and lactation routes. These results indicate that the insect-derived antigens tested may constitute potential vaccine candidates to be further evaluated. © 2010 Elsevier Ltd.
format journal article
author Alonso-Padilla, J.
Jiménez de Oya, N.
Blázquez, A. B.
Escribano-Romero, E.
Escribano, J. M.
Saiz, J. C.
spellingShingle Alonso-Padilla, J.
Jiménez de Oya, N.
Blázquez, A. B.
Escribano-Romero, E.
Escribano, J. M.
Saiz, J. C.
Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease
author_facet Alonso-Padilla, J.
Jiménez de Oya, N.
Blázquez, A. B.
Escribano-Romero, E.
Escribano, J. M.
Saiz, J. C.
author_sort Alonso-Padilla, J.
title Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease
title_short Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease
title_full Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease
title_fullStr Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease
title_full_unstemmed Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease
title_sort recombinant west nile virus envelope protein e and domain iii expressed in insect larvae protects mice against west nile disease
publishDate 2011
url http://hdl.handle.net/20.500.12792/6037
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