Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds
The SHAQKYF R1MYB transcription factor (TF) HvMYBS3 from barley is an activator of gene expression both during endosperm development and in aleurone cells upon seed germination. Its mRNA was detected as early as 10 days after flowering in developing barley endosperm, with a peak at 18 days, and in aleurone cells at 8 h after water imbibition, as shown by Northern blot and in situ hybridization analyses. The HvMYBS3 protein expressed in bacteria binds to oligonucleotides containing a GATA core derived from the promoters of (i) the developing endosperm gene ltr1 (5′-GATAAGATA-3′) encoding trypsin inhibitor BTI-CMe, and (ii) the post-germinating aleurone gene Amy6.4 (5′-TATCCAC-3′/5′-GTGGATA-3′) encoding a high-pl α-amylase. Transient expression experiments in co-bombarded developing endosperms and in barley aleurone layers demonstrated that HvMYBS3 trans-activated transcription both from ltr1 and Amy6.4 promoters, in contrast with a previously reported seed-expressed R1MYB, HvMCB1, which was an activator of ltr1 and a transcriptional repressor of the Amy6.4 gene. In the yeast three-hybrid system, the HvMYBS3 protein formed a ternary complex with BPBF and BLZ2, two important seed TFs. However, no binary interactions could be detected between HvMYBS3 and BLZ2 or between HvMYBS3 and BPBF. © 2006 The Authors.
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dig-inia-es-20.500.12792-54422020-12-15T09:55:03Z Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds Rubio-Somoza, I. Martinez, M. Abraham, Z. Diaz, I. Carbonero, P. The SHAQKYF R1MYB transcription factor (TF) HvMYBS3 from barley is an activator of gene expression both during endosperm development and in aleurone cells upon seed germination. Its mRNA was detected as early as 10 days after flowering in developing barley endosperm, with a peak at 18 days, and in aleurone cells at 8 h after water imbibition, as shown by Northern blot and in situ hybridization analyses. The HvMYBS3 protein expressed in bacteria binds to oligonucleotides containing a GATA core derived from the promoters of (i) the developing endosperm gene ltr1 (5′-GATAAGATA-3′) encoding trypsin inhibitor BTI-CMe, and (ii) the post-germinating aleurone gene Amy6.4 (5′-TATCCAC-3′/5′-GTGGATA-3′) encoding a high-pl α-amylase. Transient expression experiments in co-bombarded developing endosperms and in barley aleurone layers demonstrated that HvMYBS3 trans-activated transcription both from ltr1 and Amy6.4 promoters, in contrast with a previously reported seed-expressed R1MYB, HvMCB1, which was an activator of ltr1 and a transcriptional repressor of the Amy6.4 gene. In the yeast three-hybrid system, the HvMYBS3 protein formed a ternary complex with BPBF and BLZ2, two important seed TFs. However, no binary interactions could be detected between HvMYBS3 and BLZ2 or between HvMYBS3 and BPBF. © 2006 The Authors. 2020-10-22T20:05:51Z 2020-10-22T20:05:51Z 2006 journal article http://hdl.handle.net/20.500.12792/5442 10.1111/j.1365-313X.2006.02777.x eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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The SHAQKYF R1MYB transcription factor (TF) HvMYBS3 from barley is an activator of gene expression both during endosperm development and in aleurone cells upon seed germination. Its mRNA was detected as early as 10 days after flowering in developing barley endosperm, with a peak at 18 days, and in aleurone cells at 8 h after water imbibition, as shown by Northern blot and in situ hybridization analyses. The HvMYBS3 protein expressed in bacteria binds to oligonucleotides containing a GATA core derived from the promoters of (i) the developing endosperm gene ltr1 (5′-GATAAGATA-3′) encoding trypsin inhibitor BTI-CMe, and (ii) the post-germinating aleurone gene Amy6.4 (5′-TATCCAC-3′/5′-GTGGATA-3′) encoding a high-pl α-amylase. Transient expression experiments in co-bombarded developing endosperms and in barley aleurone layers demonstrated that HvMYBS3 trans-activated transcription both from ltr1 and Amy6.4 promoters, in contrast with a previously reported seed-expressed R1MYB, HvMCB1, which was an activator of ltr1 and a transcriptional repressor of the Amy6.4 gene. In the yeast three-hybrid system, the HvMYBS3 protein formed a ternary complex with BPBF and BLZ2, two important seed TFs. However, no binary interactions could be detected between HvMYBS3 and BLZ2 or between HvMYBS3 and BPBF. © 2006 The Authors. |
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journal article |
author |
Rubio-Somoza, I. Martinez, M. Abraham, Z. Diaz, I. Carbonero, P. |
spellingShingle |
Rubio-Somoza, I. Martinez, M. Abraham, Z. Diaz, I. Carbonero, P. Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds |
author_facet |
Rubio-Somoza, I. Martinez, M. Abraham, Z. Diaz, I. Carbonero, P. |
author_sort |
Rubio-Somoza, I. |
title |
Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds |
title_short |
Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds |
title_full |
Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds |
title_fullStr |
Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds |
title_full_unstemmed |
Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds |
title_sort |
ternary complex formation between hvmybs3 and other factors involved in transcriptional control in barley seeds |
publishDate |
2006 |
url |
http://hdl.handle.net/20.500.12792/5442 |
work_keys_str_mv |
AT rubiosomozai ternarycomplexformationbetweenhvmybs3andotherfactorsinvolvedintranscriptionalcontrolinbarleyseeds AT martinezm ternarycomplexformationbetweenhvmybs3andotherfactorsinvolvedintranscriptionalcontrolinbarleyseeds AT abrahamz ternarycomplexformationbetweenhvmybs3andotherfactorsinvolvedintranscriptionalcontrolinbarleyseeds AT diazi ternarycomplexformationbetweenhvmybs3andotherfactorsinvolvedintranscriptionalcontrolinbarleyseeds AT carbonerop ternarycomplexformationbetweenhvmybs3andotherfactorsinvolvedintranscriptionalcontrolinbarleyseeds |
_version_ |
1758004952552177664 |