Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
30 Pág.
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| Language: | English |
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Elsevier
2024-04-19
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| Subjects: | Aquaporin, Domestic ruminants, Sperm, Cryoresistance, |
| Online Access: | http://hdl.handle.net/10261/354526 http://dx.doi.org/10.13039/501100011033 |
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dig-inia-es-10261-3545262024-05-20T09:52:59Z Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat Pequeño, Belén Millán de la Blanca, María Gemma Castaño, Cristina Toledano-Díaz, A. Esteso, Milagros C. Alba, Esther Arrebola, Francisco A. Ungerfeld, Rodolfo Martínez-Madrid, Belén Álvarez-Rodríguez, Manuel Rodriguez-Martinez, Heriberto Santiago Moreno, Julián Agencia Estatal de Investigación (España) Pequeño, Belén [0000-0003-3962-1982] Millán de la Blanca, María Gemma [0000-0002-2810-8510] Castaño, Cristina [0000-0003-1134-1436] Toledano-Díaz, A. [0000-0001-6679-485X] Esteso, Milagros C. [0000-0002-8963-5736] Arrebola, Francisco A. [0000-0002-5571-7258] Ungerfeld, Rodolfo [0000-0003-4685-2105] Martínez-Madrid, Belén [0000-0001-6852-4597] Álvarez-Rodríguez, Manuel [0000-0003-0120-354X] Rodriguez-Martinez, Heriberto [0000-0002-5194-2124] Santiago Moreno, Julián [0000-0001-5551-8120] Aquaporin Domestic ruminants Sperm Cryoresistance 30 Pág. The freeze-thawing process induces osmotic changes that may affect the membrane domain location of aquaporins’ (AQP) in spermatozoa. Recent studies suggest that changes in AQP3 localization allows better sperm osmo-adaptation, improving the cryoresistance. Ultra-rapid freezing is an alternative cryopreservation technique that requires less equipment than conventional freezing, and it is faster, simpler and can be used in the field. This study aimed to determine the influence of freezing-thawing rates (slow (control) vs. ultra-rapid) on AQP3 expression and location in the spermatozoa from small ruminants (sheep and goats) and its relationship with sperm cryo-damage. Spermatozoa were collected from 10 Merino rams and 10 Murciano-Granadina bucks. The presence and distribution of AQP3 were assessed by Western blotting and immunocytochemistry (ICC), employing a commercial rabbit polyclonal antibody. Sperm motility was CASA system-analyzed, and membrane and acrosome integrity assessed by fluorescence (PI/PNA-FITC). Western blotting did not detect a significant effect of freezing-thawing rate on the amount of AQP3 while ICC found freezing-thawing rate affecting AQP3 location (P<0.05). In both species, the percentages of spermatozoa showing AQP3 in the post-acrosome region, mid-piece, and principal piece of the tail were greater in samples cryopreserved by slow freezing-thawing (control) than ultra-rapid freezing-thawing rates (P<0.05). Spermatozoa cryopreserved using ultra-rapid freezing-thawing showed decrease motility, plasma membrane, and acrosome integrity (P<0.05), which might be related, at least in part, to a lower expression of AQP3. In conclusion, the cooling rate modifies the location of AQP3 in spermatozoa of sheep and goat, which might be associated with sperm cryosurvival. This study was supported by the project PID2020-113288RB-I00/AEI/10.13039/501100011033. B. Pequeño was the recipient of a grant for pre-doctoral researchers from AEI (PRE2018-085637). Peer reviewed 2024-04-22T10:25:30Z 2024-04-22T10:25:30Z 2024-04-19 artículo http://purl.org/coar/resource_type/c_6501 Theriogenology : (2024) 0093-691X http://hdl.handle.net/10261/354526 10.1016/j.theriogenology.2024.04.008 1879-3231 http://dx.doi.org/10.13039/501100011033 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113288RB-I00/ES/MECANISMOS DE CRIO-RESISTENCIA ESPERMATICA Y NUEVOS PROCEDIMIENTOS DE CRIOPRESERVACION ESPERMATICA Y TEJIDO TESTICULAR EN RUMIANTES DOMESTICOS Y SILVESTRES/ Departamento de Reproducción Animal Postprint https://doi.org/10.1016/j.theriogenology.2024.04.008 Sí open application/pdf Elsevier |
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Aquaporin Domestic ruminants Sperm Cryoresistance Aquaporin Domestic ruminants Sperm Cryoresistance |
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Aquaporin Domestic ruminants Sperm Cryoresistance Aquaporin Domestic ruminants Sperm Cryoresistance Pequeño, Belén Millán de la Blanca, María Gemma Castaño, Cristina Toledano-Díaz, A. Esteso, Milagros C. Alba, Esther Arrebola, Francisco A. Ungerfeld, Rodolfo Martínez-Madrid, Belén Álvarez-Rodríguez, Manuel Rodriguez-Martinez, Heriberto Santiago Moreno, Julián Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| description |
30 Pág. |
| author2 |
Agencia Estatal de Investigación (España) |
| author_facet |
Agencia Estatal de Investigación (España) Pequeño, Belén Millán de la Blanca, María Gemma Castaño, Cristina Toledano-Díaz, A. Esteso, Milagros C. Alba, Esther Arrebola, Francisco A. Ungerfeld, Rodolfo Martínez-Madrid, Belén Álvarez-Rodríguez, Manuel Rodriguez-Martinez, Heriberto Santiago Moreno, Julián |
| format |
artículo |
| topic_facet |
Aquaporin Domestic ruminants Sperm Cryoresistance |
| author |
Pequeño, Belén Millán de la Blanca, María Gemma Castaño, Cristina Toledano-Díaz, A. Esteso, Milagros C. Alba, Esther Arrebola, Francisco A. Ungerfeld, Rodolfo Martínez-Madrid, Belén Álvarez-Rodríguez, Manuel Rodriguez-Martinez, Heriberto Santiago Moreno, Julián |
| author_sort |
Pequeño, Belén |
| title |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title_short |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title_full |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title_fullStr |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title_full_unstemmed |
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| title_sort |
cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat |
| publisher |
Elsevier |
| publishDate |
2024-04-19 |
| url |
http://hdl.handle.net/10261/354526 http://dx.doi.org/10.13039/501100011033 |
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