Application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein
8 Pág.
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier BV
2020-04-22
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| Subjects: | 5-hydroxy-equol, Bifidobacterium, Daidzein reductase, Dihydrodaidzein, Dihydrogenistein, Equol, Lactic acid bacteria, Soy beverage, |
| Online Access: | http://hdl.handle.net/10261/321788 https://api.elsevier.com/content/abstract/scopus_id/85083654958 |
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dig-inia-es-10261-3217882024-05-15T20:38:46Z Application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein Peirotén, Ángela Gaya Sicilia, María Pilar Landete, José María Ministerio de Ciencia, Innovación y Universidades (España) Peirotén, Ángela [0000-0002-1532-8530] Gaya Sicilia, María Pilar [0000-0003-1172-3119] Landete, José María [0000-0002-5147-3989] 5-hydroxy-equol Bifidobacterium Daidzein reductase Dihydrodaidzein Dihydrogenistein Equol Lactic acid bacteria Soy beverage 8 Pág. Dihydrodaidzein (DHD) and dihydrogenistein (DHG) are intermediate compounds in the production of equol and 5-hydroxy-equol from daidzein and genistein by certain intestinal bacteria. In this work, we explored the heterologous expression of the daidzein reductase gene from Slackia isoflavoniconvertens DSM22006, responsible for the formation of DHD and DHG, in nine lactic acid bacteria and Bifidobacterium strains under a strong constitutive promoter in the plasmid pNZ:TuR.dzr. All the transformed strains showed high production of DHD and DHG both from pure daidzein and genistein and from the isoflavone glycosides present in soy beverage. In addition, Lactococcus lactis MG1363 pNZ:TuR.dzr, expressing the recombinant daidzein reductase, incremented the production of equol by equol-producing intestinal microbiotas in a colonic environment. Nevertheless, other recombinant strains tested showed the opposite effect to L. lactis MG1363 pNZ:TuR.dzr in the production of equol by intestinal microbiota. Here, we describe for the first time the production of 5-hydroxy-equol by human intestinal microbiota, both from genistein and DHG. The use of DHD and DHG as substrates, compared to daidzein and genistein, resulted in an increment of equol and 5-hydroxy-equol production by intestinal microbiota. The recombinant strains developed would be of value for the development of fermented soy beverage enriched in DHD and DHG with the aim of facilitate equol and 5-hydroxy-equol production by intestinal microbiota. This work was supported by project RTA2017-00002-00-00 from the Spanish Ministry of Science, Innovation and University. Peer reviewed 2023-06-21T07:39:27Z 2023-06-21T07:39:27Z 2020-04-22 artículo http://purl.org/coar/resource_type/c_6501 Food Research International 134: e109257 (2020) 0963-9969 http://hdl.handle.net/10261/321788 10.1016/j.foodres.2020.109257 1873-7145 32517924 2-s2.0-85083654958 https://api.elsevier.com/content/abstract/scopus_id/85083654958 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICIU//RTA2017-00002-00-00 Departamento de Tecnología de Alimentos Publisher's version https://doi.org/10.1016/j.foodres.2020.109257 Sí open application/pdf Elsevier BV |
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5-hydroxy-equol Bifidobacterium Daidzein reductase Dihydrodaidzein Dihydrogenistein Equol Lactic acid bacteria Soy beverage 5-hydroxy-equol Bifidobacterium Daidzein reductase Dihydrodaidzein Dihydrogenistein Equol Lactic acid bacteria Soy beverage |
| spellingShingle |
5-hydroxy-equol Bifidobacterium Daidzein reductase Dihydrodaidzein Dihydrogenistein Equol Lactic acid bacteria Soy beverage 5-hydroxy-equol Bifidobacterium Daidzein reductase Dihydrodaidzein Dihydrogenistein Equol Lactic acid bacteria Soy beverage Peirotén, Ángela Gaya Sicilia, María Pilar Landete, José María Application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein |
| description |
8 Pág. |
| author2 |
Ministerio de Ciencia, Innovación y Universidades (España) |
| author_facet |
Ministerio de Ciencia, Innovación y Universidades (España) Peirotén, Ángela Gaya Sicilia, María Pilar Landete, José María |
| format |
artículo |
| topic_facet |
5-hydroxy-equol Bifidobacterium Daidzein reductase Dihydrodaidzein Dihydrogenistein Equol Lactic acid bacteria Soy beverage |
| author |
Peirotén, Ángela Gaya Sicilia, María Pilar Landete, José María |
| author_sort |
Peirotén, Ángela |
| title |
Application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein |
| title_short |
Application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein |
| title_full |
Application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein |
| title_fullStr |
Application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein |
| title_full_unstemmed |
Application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein |
| title_sort |
application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein |
| publisher |
Elsevier BV |
| publishDate |
2020-04-22 |
| url |
http://hdl.handle.net/10261/321788 https://api.elsevier.com/content/abstract/scopus_id/85083654958 |
| work_keys_str_mv |
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| _version_ |
1802819750135332864 |