Analysis of Benzalkonium Chloride Resistance and Potential Virulence of Listeria monocytogenes Isolates Obtained from Different Stages of a Poultry Production Chain in Spain

Analysis of Benzalkonium Chloride Resistance and Potential Virulence of Listeria monocytogenes Isolates Obtained from Different Stages of a Poultry Production Chain in Spain

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Main Authors: López, Victoria, Ortiz, Sagrario, Corujo, Alfredo, Martínez Suarez, Joaquín V.
Other Authors: Ministerio de Ciencia, Innovación y Universidades (España)
Format: artículo biblioteca
Language:English
Published: Elsevier BV 2020-03-01
Subjects:Listeria monocytogenes, Biocide resistance, Potential virulence, Poultry,
Online Access:http://hdl.handle.net/10261/311677
http://dx.doi.org/10.13039/501100000780
https://api.elsevier.com/content/abstract/scopus_id/85079334353
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spelling dig-inia-es-10261-3116772024-05-21T20:36:31Z Analysis of Benzalkonium Chloride Resistance and Potential Virulence of Listeria monocytogenes Isolates Obtained from Different Stages of a Poultry Production Chain in Spain López, Victoria Ortiz, Sagrario Corujo, Alfredo Martínez Suarez, Joaquín V. Ministerio de Ciencia, Innovación y Universidades (España) European Commission Nutreco López, Victoria [0000-0001-8129-8352] Martínez Suarez, Joaquín V. [0000-0003-4306-3223] Listeria monocytogenes Biocide resistance Potential virulence Poultry 9 Pág. Listeria monocytogenes can survive in food production facilities and can be transmitted via contamination of food during the various stages of food production. This study was conducted to compile the results of three independent previous studies on the genetic diversity of L. monocytogenes in a poultry production company in Spain and to determine the potential virulence and sanitizer resistance of the strains by using both genotype and phenotype analyses. L. monocytogenes was detected at three production stages: a broiler abattoir, a processing plant, and retail stores marketing fresh poultry products from the same company. These three stages spanned three locations in three provinces of Spain. A set of 347 L. monocytogenes isolates representing 39 subtypes was obtained using pulsed-field gel electrophoresis (PFGE). A total of 28 subtypes (68%) had a full-length internalin A gene, and two subtypes had a phenotype with low potential for virulence because of a mutation in the prfA gene. A total of 32 subtypes (82%) were classified as benzalkonium chloride resistant (BAC-R) and contained the resistance determinant bcrABC (21 subtypes, 54%) or the resistance gene qacH (11 subtypes, 28%). A total of 13 persistent BAC-R subtypes (minimum of 3 months between the first and last sample from with the isolate was recovered) were identified at the abattoir and processing plant. The three production stages shared a unique subtype (PFGE type 1), which had the mutation in the prfA gene and the bcrABC resistance determinant. Whole genome sequencing revealed this subtype to be sequence type 31. Limited genetic diversity was noted in the isolates studied, including some subtypes that were persistent in the environment of the investigated facilities. Given the high prevalence of BAC-R subtypes, these results support the association between resistance to biocides and persistence of L. monocytogenes. This work was supported in part by research project grants TA2014-00045-C03-01 and RTI2018-098267-R-C31 (INIA FEDER) from the Spanish Ministry of Science, Innovation and Universities. The support of Nutreco (Amersfoort, The Netherlands) is also gratefully acknowledged. Peer reviewed 2023-06-16T07:56:17Z 2023-06-16T07:56:17Z 2020-03-01 artículo http://purl.org/coar/resource_type/c_6501 Journal of Food Protection 83(3): 443-451 (2020) 0362-028X http://hdl.handle.net/10261/311677 10.4315/0362-028X.JFP-19-289 1944-9097 http://dx.doi.org/10.13039/501100000780 32053831 2-s2.0-85079334353 https://api.elsevier.com/content/abstract/scopus_id/85079334353 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//RTA2014-00045-C03-01/ES/Supervivencia de Listeria monocytogenes sobre superficies de contacto con alimentos: un abordaje multidisciplinar de un problema complejo/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098267-R-C31/ES/ALTERNATIVAS AL USO DE DESINFECTANTES EN LA INDUSTRIA ALIMENTARIA DIRIGIDAS A REDUCIR LA SUPERVIVENCIA DE LISTERIA MONOCYTOGENES Y SALMONELLA ENTERICA SOBRE LAS SUPERFICIES/ Departamento de Tecnología de Alimentos​​ Publisher's version https://doi.org/10.4315/0362-028X.JFP-19-289 Sí open application/pdf Elsevier BV
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Listeria monocytogenes
Biocide resistance
Potential virulence
Poultry
Listeria monocytogenes
Biocide resistance
Potential virulence
Poultry
spellingShingle Listeria monocytogenes
Biocide resistance
Potential virulence
Poultry
Listeria monocytogenes
Biocide resistance
Potential virulence
Poultry
López, Victoria
Ortiz, Sagrario
Corujo, Alfredo
Martínez Suarez, Joaquín V.
Analysis of Benzalkonium Chloride Resistance and Potential Virulence of Listeria monocytogenes Isolates Obtained from Different Stages of a Poultry Production Chain in Spain
description 9 Pág.
author2 Ministerio de Ciencia, Innovación y Universidades (España)
author_facet Ministerio de Ciencia, Innovación y Universidades (España)
López, Victoria
Ortiz, Sagrario
Corujo, Alfredo
Martínez Suarez, Joaquín V.
format artículo
topic_facet Listeria monocytogenes
Biocide resistance
Potential virulence
Poultry
author López, Victoria
Ortiz, Sagrario
Corujo, Alfredo
Martínez Suarez, Joaquín V.
author_sort López, Victoria
title Analysis of Benzalkonium Chloride Resistance and Potential Virulence of Listeria monocytogenes Isolates Obtained from Different Stages of a Poultry Production Chain in Spain
title_short Analysis of Benzalkonium Chloride Resistance and Potential Virulence of Listeria monocytogenes Isolates Obtained from Different Stages of a Poultry Production Chain in Spain
title_full Analysis of Benzalkonium Chloride Resistance and Potential Virulence of Listeria monocytogenes Isolates Obtained from Different Stages of a Poultry Production Chain in Spain
title_fullStr Analysis of Benzalkonium Chloride Resistance and Potential Virulence of Listeria monocytogenes Isolates Obtained from Different Stages of a Poultry Production Chain in Spain
title_full_unstemmed Analysis of Benzalkonium Chloride Resistance and Potential Virulence of Listeria monocytogenes Isolates Obtained from Different Stages of a Poultry Production Chain in Spain
title_sort analysis of benzalkonium chloride resistance and potential virulence of listeria monocytogenes isolates obtained from different stages of a poultry production chain in spain
publisher Elsevier BV
publishDate 2020-03-01
url http://hdl.handle.net/10261/311677
http://dx.doi.org/10.13039/501100000780
https://api.elsevier.com/content/abstract/scopus_id/85079334353
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