Functional and Phenotypic Characterization of B Cells in the Teleost Adipose Tissue

Functional and Phenotypic Characterization of B Cells in the Teleost Adipose Tissue

15 Pág.

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Bibliographic Details
Main Authors: Simón, Rocío, Martín-Martín, Alba, Morel, Esther, Díaz-Rosales, Patricia, Tafalla, Carolina
Other Authors: European Commission
Format: artículo biblioteca
Language:English
Published: Frontiers Media 2022-05-10
Subjects:B cells, IgM, Adipose tissue, Peritoneal antigens, Rainbow trout, Teleost fish,
Online Access:http://hdl.handle.net/10261/272864
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/100012818
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spelling dig-inia-es-10261-2728642022-07-16T01:15:25Z Functional and Phenotypic Characterization of B Cells in the Teleost Adipose Tissue Simón, Rocío Martín-Martín, Alba Morel, Esther Díaz-Rosales, Patricia Tafalla, Carolina European Commission Comunidad de Madrid Simón, Rocío [0000-0001-6584-5141] Martín-Martín, Alba [0000-0003-3092-2893] Morel, Esther [0000-0002-4768-082X] Díaz-Rosales, Patricia [0000-0001-6965-3702] Tafalla, Carolina [0000-0002-0860-2976] B cells IgM Adipose tissue Peritoneal antigens Rainbow trout Teleost fish 15 Pág. The immune response of the adipose tissue (AT) has been neglected in most animal models until investigations in human and mice linked obesity to chronic inflammation, highlighting the immune nature of this tissue. Despite this, in teleost fish, only a few studies have addressed the immune role of the AT. These studies have mostly focused on reporting transcriptional changes in the AT in response to diverse intraperitoneally delivered stimuli. Although the presence of B cells within the AT was also previously revealed, these cells have never been phenotypically or functionally characterized and this is what we have addressed in the current study. Initially, the B cell populations present in the rainbow trout (Oncorhynchus mykiss) AT were characterized in comparison to B cells from other sources. As occurs in other rainbow trout tissues, IgM+IgD+, IgM+IgD- and IgD+IgM- B cell subsets were identified in the AT. Interestingly, AT IgM+IgD- B cells showed a transcriptional profile that agrees with that of cells that have committed to plasmablasts/plasma cells, being this profile much more pronounced towards a differentiation state than that of blood IgM+IgD- B cells. Accordingly, the IgM-secreting capacity of AT B cells is significantly higher than that of blood B cells. Additionally, AT IgM+IgD+ B cells also showed specific phenotypic traits when compared to their counterparts in other tissues. Finally, we established how these B cell subsets responded when rainbow trout were intraperitoneally injected with a model antigen. Our results demonstrate that the AT hosts plasmablasts/plasma cells that secrete specific IgMs, as happens in the peritoneal cavity and systemic immune tissues. Although the presence of these antigen-specific IgM-secreting cells was more abundant in the peritoneal cavity, these specific differentiated B cells were detected in the AT for long time periods at levels similar to those of spleen and head kidney. Our results provide new evidence regarding the immune role of the teleost AT, indicating that it functions as a secondary lymphoid organ that promotes immunity to peritoneal antigens. This work was supported by the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM) and by the Comunidad de Madrid (grant 2016-T1/BIO-1672). Peer reviewed 2022-06-20T08:18:59Z 2022-06-20T08:18:59Z 2022-05-10 artículo Frontiers in Immunology 13: 868551(2022) 1664-3224 http://hdl.handle.net/10261/272864 10.3389/fimmu.2022.868551 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 35619704 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/725061/TEMUBLYM S2016-T1/BIO-1672 Frontiers in immunology Publisher's version https://doi.org/10.3389/fimmu.2022.868551 Sí open Frontiers Media
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic B cells
IgM
Adipose tissue
Peritoneal antigens
Rainbow trout
Teleost fish
B cells
IgM
Adipose tissue
Peritoneal antigens
Rainbow trout
Teleost fish
spellingShingle B cells
IgM
Adipose tissue
Peritoneal antigens
Rainbow trout
Teleost fish
B cells
IgM
Adipose tissue
Peritoneal antigens
Rainbow trout
Teleost fish
Simón, Rocío
Martín-Martín, Alba
Morel, Esther
Díaz-Rosales, Patricia
Tafalla, Carolina
Functional and Phenotypic Characterization of B Cells in the Teleost Adipose Tissue
description 15 Pág.
author2 European Commission
author_facet European Commission
Simón, Rocío
Martín-Martín, Alba
Morel, Esther
Díaz-Rosales, Patricia
Tafalla, Carolina
format artículo
topic_facet B cells
IgM
Adipose tissue
Peritoneal antigens
Rainbow trout
Teleost fish
author Simón, Rocío
Martín-Martín, Alba
Morel, Esther
Díaz-Rosales, Patricia
Tafalla, Carolina
author_sort Simón, Rocío
title Functional and Phenotypic Characterization of B Cells in the Teleost Adipose Tissue
title_short Functional and Phenotypic Characterization of B Cells in the Teleost Adipose Tissue
title_full Functional and Phenotypic Characterization of B Cells in the Teleost Adipose Tissue
title_fullStr Functional and Phenotypic Characterization of B Cells in the Teleost Adipose Tissue
title_full_unstemmed Functional and Phenotypic Characterization of B Cells in the Teleost Adipose Tissue
title_sort functional and phenotypic characterization of b cells in the teleost adipose tissue
publisher Frontiers Media
publishDate 2022-05-10
url http://hdl.handle.net/10261/272864
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/100012818
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AT tafallacarolina functionalandphenotypiccharacterizationofbcellsintheteleostadiposetissue
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