Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440

Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440

18 pages, 5 tables, 5 figures.-- PMID: 12534466 [PubMed].

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Main Authors: Jiménez Zarco, José Ignacio, Miñambres Rodríguez, Baltasar, García, José Luis, Díaz, Eduardo
Format: artículo biblioteca
Language:English
Published: Blackwell Publishing 2002
Subjects:Tol plasmid pwwo, Sp strain hr199, Gene-cluster, Biotechnological applications, Transcriptional activator, Agrobacterium-tumefaciens, Phenylalanine-hydroxylase, Sinorhizobium-meliloti, Aspergillus-nidulans, Escherichia-coli,
Online Access:http://hdl.handle.net/10261/12160
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spelling dig-igm-es-10261-121602020-11-23T11:35:45Z Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440 Jiménez Zarco, José Ignacio Miñambres Rodríguez, Baltasar García, José Luis Díaz, Eduardo Tol plasmid pwwo Sp strain hr199 Gene-cluster Biotechnological applications Transcriptional activator Agrobacterium-tumefaciens Phenylalanine-hydroxylase Sinorhizobium-meliloti Aspergillus-nidulans Escherichia-coli 18 pages, 5 tables, 5 figures.-- PMID: 12534466 [PubMed]. Analysis of the catabolic potential of Pseudomonas putida KT2440 against a wide range of natural aromatic compounds and sequence comparisons with the entire genome of this microorganism predicted the existence of at least four main pathways for the catabolism of central aromatic intermediates, that is, the protocatechuate (pca genes) and catechol (cat genes) branches of the beta-ketoadipate pathway, the homogentisate pathway (hmg/fah/mai genes) and the phenylacetate pathway (pha genes). Two additional gene clusters that might be involved in the catabolism of N-heterocyclic aromatic compounds (nic cluster) and in a central meta-cleavage pathway (pcm genes) were also identified. Furthermore, the genes encoding the peripheral pathways for the catabolism of p-hydroxybenzoate (pob), benzoate (ben), quinate (qui), phenylpropenoid compounds (fcs, ech, vdh, cal, van, acd and acs), phenylalanine and tyrosine (phh, hpd) and n-phenylalkanoic acids (fad) were mapped in the chromosome of P. putida KT2440. Although a repetitive extragenic palindromic (REP) element is usually associated with the gene clusters, a supraoperonic clustering of catabolic genes that channel different aromatic compounds into a common central pathway (catabolic island) was not observed in P. putida KT2440. The global view on the mineralization of aromatic compounds by P. putida KT2440 will facilitate the rational manipulation of this strain for improving biodegradation/biotransformation processes, and reveals this bacterium as a useful model system for studying biochemical, genetic, evolutionary and ecological aspects of the catabolism of aromatic compounds. This work was supported by EU contract QLK3-CT2000-00170, by the Spanish Ministry of Science and Technology (MCYT) [Red del Consejo Superior de Investigaciones Científicas (CSIC) de Biorremediación y Fitorremediación] and by grants BMC2000-0125-CO4-02 and GEN2001-4698-C05-02 from the Comisión Interministerial de Ciencia y Tecnología. J.-I.J. was the recipient of a I3P predoctoral fellowship from the CSIC. Peer reviewed 2009-04-07T10:45:15Z 2009-04-07T10:45:15Z 2002 artículo http://purl.org/coar/resource_type/c_6501 Environmental Microbiology 4(12): 824-841 (2002) 1462-2912 http://hdl.handle.net/10261/12160 10.1046/j.1462-2920.2002.00370.x en http://dx.doi.org/10.1046/j.1462-2920.2002.00370.x none 94510 bytes application/pdf Blackwell Publishing
institution IGM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-igm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IGM España
language English
topic Tol plasmid pwwo
Sp strain hr199
Gene-cluster
Biotechnological applications
Transcriptional activator
Agrobacterium-tumefaciens
Phenylalanine-hydroxylase
Sinorhizobium-meliloti
Aspergillus-nidulans
Escherichia-coli
Tol plasmid pwwo
Sp strain hr199
Gene-cluster
Biotechnological applications
Transcriptional activator
Agrobacterium-tumefaciens
Phenylalanine-hydroxylase
Sinorhizobium-meliloti
Aspergillus-nidulans
Escherichia-coli
spellingShingle Tol plasmid pwwo
Sp strain hr199
Gene-cluster
Biotechnological applications
Transcriptional activator
Agrobacterium-tumefaciens
Phenylalanine-hydroxylase
Sinorhizobium-meliloti
Aspergillus-nidulans
Escherichia-coli
Tol plasmid pwwo
Sp strain hr199
Gene-cluster
Biotechnological applications
Transcriptional activator
Agrobacterium-tumefaciens
Phenylalanine-hydroxylase
Sinorhizobium-meliloti
Aspergillus-nidulans
Escherichia-coli
Jiménez Zarco, José Ignacio
Miñambres Rodríguez, Baltasar
García, José Luis
Díaz, Eduardo
Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
description 18 pages, 5 tables, 5 figures.-- PMID: 12534466 [PubMed].
format artículo
topic_facet Tol plasmid pwwo
Sp strain hr199
Gene-cluster
Biotechnological applications
Transcriptional activator
Agrobacterium-tumefaciens
Phenylalanine-hydroxylase
Sinorhizobium-meliloti
Aspergillus-nidulans
Escherichia-coli
author Jiménez Zarco, José Ignacio
Miñambres Rodríguez, Baltasar
García, José Luis
Díaz, Eduardo
author_facet Jiménez Zarco, José Ignacio
Miñambres Rodríguez, Baltasar
García, José Luis
Díaz, Eduardo
author_sort Jiménez Zarco, José Ignacio
title Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
title_short Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
title_full Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
title_fullStr Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
title_full_unstemmed Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
title_sort genomic analysis of the aromatic catabolic pathways from pseudomonas putida kt2440
publisher Blackwell Publishing
publishDate 2002
url http://hdl.handle.net/10261/12160
work_keys_str_mv AT jimenezzarcojoseignacio genomicanalysisofthearomaticcatabolicpathwaysfrompseudomonasputidakt2440
AT minambresrodriguezbaltasar genomicanalysisofthearomaticcatabolicpathwaysfrompseudomonasputidakt2440
AT garciajoseluis genomicanalysisofthearomaticcatabolicpathwaysfrompseudomonasputidakt2440
AT diazeduardo genomicanalysisofthearomaticcatabolicpathwaysfrompseudomonasputidakt2440
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