Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus
Recombinant insect nucleopolyhedroviruses lacking the egt gene generally kill their hosts faster than wild-type strains, but the response of insects to mixtures of virus genotypes is less well known. Here, we compared the survival time, lethal dose and occlusion body yield in third instar larvae of Helicoverpa armigera (Hübner) after challenge with wild-type H. armigera SNPV (HaSNPV-wt), a strain with a deletion of the egt gene, HaSNPV-LM2, and a 1:1 mixture of these two virus strains. A range of doses was used to determine whether the total number of OBs influenced the response to challenge with a mixture of virus strains versus single strains. At high virus doses, HaSNPV-LM2 killed H. armigera larvae significantly faster (ca. 20 h) than HaSNPV-wt, but at low doses, there was no significant difference in survival time between the viruses. The survival time after challenge with mixed virus inoculum was significantly different from and intermediate between that of the single viruses at high doses, and not different from that of the single viruses at low doses. No differences in lethal dose were found between single and mixed infections or between virus genotypes. The number of occlusion bodies produced per larva increased with time to death and decreased with virus dose, but no significant differences among virus types were found.
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Academic Press
2010
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Subjects: | Mixed infection, Genetically modified baculovirus, HaSNPV, Helicoverpa armigera, Dose range, |
Online Access: | http://hdl.handle.net/10261/97496 |
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dig-idab-es-10261-974962016-09-07T09:43:16Z Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus Georgievska, Liljana Hoover, Kelli Werf, Wopke van der Muñoz, Delia Caballero, Primitivo Cory, Jenny S. Vlak, Just M. Mixed infection Genetically modified baculovirus HaSNPV Helicoverpa armigera Dose range Recombinant insect nucleopolyhedroviruses lacking the egt gene generally kill their hosts faster than wild-type strains, but the response of insects to mixtures of virus genotypes is less well known. Here, we compared the survival time, lethal dose and occlusion body yield in third instar larvae of Helicoverpa armigera (Hübner) after challenge with wild-type H. armigera SNPV (HaSNPV-wt), a strain with a deletion of the egt gene, HaSNPV-LM2, and a 1:1 mixture of these two virus strains. A range of doses was used to determine whether the total number of OBs influenced the response to challenge with a mixture of virus strains versus single strains. At high virus doses, HaSNPV-LM2 killed H. armigera larvae significantly faster (ca. 20 h) than HaSNPV-wt, but at low doses, there was no significant difference in survival time between the viruses. The survival time after challenge with mixed virus inoculum was significantly different from and intermediate between that of the single viruses at high doses, and not different from that of the single viruses at low doses. No differences in lethal dose were found between single and mixed infections or between virus genotypes. The number of occlusion bodies produced per larva increased with time to death and decreased with virus dose, but no significant differences among virus types were found. L.G. was supported by a PhD fellowship from NWO-WOTRO (Netherlands Foundation for Advancement in Tropical Research), Grant W 83-180 and by an Erasmus-Socrates grant for a stay at UPNA, Pamplona, Spain. K.H. was supported by a senior visitor’s Grant from Wageningen University Graduate School PE&RC. We thank Jacques Withagen for statistical advice. Peer Reviewed 2014-06-02T06:53:40Z 2014-06-02T06:53:40Z 2010 2014-06-02T06:53:40Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.jip.2010.01.008 issn: 0022-2011 Journal of Invertebrate Pathology 104(1): 44-50 (2010) http://hdl.handle.net/10261/97496 10.1016/j.jip.2010.01.008 none Academic Press |
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Mixed infection Genetically modified baculovirus HaSNPV Helicoverpa armigera Dose range Mixed infection Genetically modified baculovirus HaSNPV Helicoverpa armigera Dose range |
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Mixed infection Genetically modified baculovirus HaSNPV Helicoverpa armigera Dose range Mixed infection Genetically modified baculovirus HaSNPV Helicoverpa armigera Dose range Georgievska, Liljana Hoover, Kelli Werf, Wopke van der Muñoz, Delia Caballero, Primitivo Cory, Jenny S. Vlak, Just M. Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus |
description |
Recombinant insect nucleopolyhedroviruses lacking the egt gene generally kill their hosts faster than wild-type strains, but the response of insects to mixtures of virus genotypes is less well known. Here, we compared the survival time, lethal dose and occlusion body yield in third instar larvae of Helicoverpa armigera (Hübner) after challenge with wild-type H. armigera SNPV (HaSNPV-wt), a strain with a deletion of the egt gene, HaSNPV-LM2, and a 1:1 mixture of these two virus strains. A range of doses was used to determine whether the total number of OBs influenced the response to challenge with a mixture of virus strains versus single strains. At high virus doses, HaSNPV-LM2 killed H. armigera larvae significantly faster (ca. 20 h) than HaSNPV-wt, but at low doses, there was no significant difference in survival time between the viruses. The survival time after challenge with mixed virus inoculum was significantly different from and intermediate between that of the single viruses at high doses, and not different from that of the single viruses at low doses. No differences in lethal dose were found between single and mixed infections or between virus genotypes. The number of occlusion bodies produced per larva increased with time to death and decreased with virus dose, but no significant differences among virus types were found. |
format |
artículo |
topic_facet |
Mixed infection Genetically modified baculovirus HaSNPV Helicoverpa armigera Dose range |
author |
Georgievska, Liljana Hoover, Kelli Werf, Wopke van der Muñoz, Delia Caballero, Primitivo Cory, Jenny S. Vlak, Just M. |
author_facet |
Georgievska, Liljana Hoover, Kelli Werf, Wopke van der Muñoz, Delia Caballero, Primitivo Cory, Jenny S. Vlak, Just M. |
author_sort |
Georgievska, Liljana |
title |
Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus |
title_short |
Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus |
title_full |
Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus |
title_fullStr |
Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus |
title_full_unstemmed |
Dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of Helicoverpa armigera nucleopolyhedrovirus |
title_sort |
dose dependency of time to death in single and mixed infections with a wildtype and egt deletion strain of helicoverpa armigera nucleopolyhedrovirus |
publisher |
Academic Press |
publishDate |
2010 |
url |
http://hdl.handle.net/10261/97496 |
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