Direct enantiomeric analysis of Mentha essential oils
A rapid and fully automated screening of chiral compounds in essential oils, aimed to the selection of natural sources of pure enantiomers of limonene and carvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatography and mass spectrometry (RPLC-GC-MS). Essential oils obtained from Mentha spicata and Mentha piperita were analysed by direct injection into RPLC. The reported procedure includes fractionation and clean-up in RPLC, selection of the fraction to be transferred from RPLC to GC, trapping and concentration of the target compounds in the interface, thermal desorption and, finally, enantiomeric resolution and identification of chiral compounds by GC-MS. The presence of (S)-limonene and (R)-carvone as the unique enantiomeric forms existing for both compounds could be unambiguously established by transferring different volume fractions from RPLC to GC. Data obtained demonstrate high separation efficiency and well tunable selectivity in the on-line coupled RPLC-GC-MS analysis of chiral compounds. © 2013 Elsevier Ltd. All rights reserved.
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dig-ictan-es-10261-875502016-12-21T08:46:07Z Direct enantiomeric analysis of Mentha essential oils Barba, Carmen Santa-María, Guillermo Herraiz Carasa, Marta Martínez, Rosa M. A rapid and fully automated screening of chiral compounds in essential oils, aimed to the selection of natural sources of pure enantiomers of limonene and carvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatography and mass spectrometry (RPLC-GC-MS). Essential oils obtained from Mentha spicata and Mentha piperita were analysed by direct injection into RPLC. The reported procedure includes fractionation and clean-up in RPLC, selection of the fraction to be transferred from RPLC to GC, trapping and concentration of the target compounds in the interface, thermal desorption and, finally, enantiomeric resolution and identification of chiral compounds by GC-MS. The presence of (S)-limonene and (R)-carvone as the unique enantiomeric forms existing for both compounds could be unambiguously established by transferring different volume fractions from RPLC to GC. Data obtained demonstrate high separation efficiency and well tunable selectivity in the on-line coupled RPLC-GC-MS analysis of chiral compounds. © 2013 Elsevier Ltd. All rights reserved. Peer Reviewed 2013-11-26T10:58:12Z 2013-11-26T10:58:12Z 2013 2013-11-26T10:58:13Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.foodchem.2013.03.013 issn: 0308-8146 Food Chemistry 141: 542- 547 (2013) http://hdl.handle.net/10261/87550 10.1016/j.foodchem.2013.03.013 en none Elsevier |
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A rapid and fully automated screening of chiral compounds in essential oils, aimed to the selection of natural sources of pure enantiomers of limonene and carvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatography and mass spectrometry (RPLC-GC-MS). Essential oils obtained from Mentha spicata and Mentha piperita were analysed by direct injection into RPLC. The reported procedure includes fractionation and clean-up in RPLC, selection of the fraction to be transferred from RPLC to GC, trapping and concentration of the target compounds in the interface, thermal desorption and, finally, enantiomeric resolution and identification of chiral compounds by GC-MS. The presence of (S)-limonene and (R)-carvone as the unique enantiomeric forms existing for both compounds could be unambiguously established by transferring different volume fractions from RPLC to GC. Data obtained demonstrate high separation efficiency and well tunable selectivity in the on-line coupled RPLC-GC-MS analysis of chiral compounds. © 2013 Elsevier Ltd. All rights reserved. |
format |
artículo |
author |
Barba, Carmen Santa-María, Guillermo Herraiz Carasa, Marta Martínez, Rosa M. |
spellingShingle |
Barba, Carmen Santa-María, Guillermo Herraiz Carasa, Marta Martínez, Rosa M. Direct enantiomeric analysis of Mentha essential oils |
author_facet |
Barba, Carmen Santa-María, Guillermo Herraiz Carasa, Marta Martínez, Rosa M. |
author_sort |
Barba, Carmen |
title |
Direct enantiomeric analysis of Mentha essential oils |
title_short |
Direct enantiomeric analysis of Mentha essential oils |
title_full |
Direct enantiomeric analysis of Mentha essential oils |
title_fullStr |
Direct enantiomeric analysis of Mentha essential oils |
title_full_unstemmed |
Direct enantiomeric analysis of Mentha essential oils |
title_sort |
direct enantiomeric analysis of mentha essential oils |
publisher |
Elsevier |
publishDate |
2013 |
url |
http://hdl.handle.net/10261/87550 |
work_keys_str_mv |
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