IGF-I and IGF-II mRNA expression in primary cultures of fetal hepatocytes
[EN] Secretion of IGFs in adult animals is mainly regulated by growth hormone (GH), but the role of other factors becomes principal during the perinatal period of the rat, when the IGF response to GH is not yet fully established. "In vitro" models overcome the simultaneous fluctuations of fuels and hormones that occur in live animals and are useful to investigate the underlying mechanisms by which hormones and nutrients regulate IGF secretion. A selective primary culture of fetal rat hepatocytes has been established in our laboratory in order to elucidate the molecular mechanism of action of different factors and conditions on IGF-I and -II gene expression during the perinatal period of the rat. In this preliminary study we report the regulatory role of serum on IGF-I and -II gene expression during stages of perinatal development. This system should be a useful tool for further studies of molecular mechanisms of IGF-I and -II regulation.
| Main Authors: | , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Universidad de Granada
1996
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| Subjects: | IGFs regulation, Serum factors, Fetal hepatocyte culture, Regulación de IGFs, Factores séricos, Cultivo de hepatocitos fetales, |
| Online Access: | http://hdl.handle.net/10261/190107 |
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| Summary: | [EN] Secretion of IGFs in adult animals is mainly regulated by growth hormone (GH), but the role of other factors becomes principal during the perinatal period of the rat, when the IGF response to GH is not yet fully established. "In vitro" models overcome the simultaneous fluctuations of fuels and hormones that occur in live animals and are useful to investigate the underlying mechanisms by which hormones and nutrients regulate IGF secretion. A selective primary culture of fetal rat hepatocytes has been established in our laboratory in order to elucidate the molecular mechanism of action of different factors and conditions on IGF-I and -II gene expression during the perinatal period of the rat. In this preliminary study we report the regulatory role of serum on IGF-I and -II gene expression during stages of perinatal development. This system should be a useful tool for further studies of molecular mechanisms of IGF-I and -II regulation. |
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