A Metagenomic and Amplicon Sequencing Combined Approach Reveals the Best Primers to Study Marine Aerobic Anoxygenic Phototrophs
12 pages, 3 figures, 1 table, supplementary information https://doi.org/10.1007/s00248-023-02220-y.-- Data Availability: Most of the analyses were performed in R version 4.2.0 (R Core Team 2022), and code is available in the following repository: https://gitlab.com/crgazulla/analysing-pufm-primers-for-marine-aap-studies. The pufM database is available in the Supplementary Material. Amplicon sequences have been deposited in the NCBI Sequence Read Archive (SRA) under BioProject ID PRJNA919028. Sequences from the BBMO metagenomic dataset were published in Auladell et al., [28], and those from the Malaspina Expedition dataset are deposited under BioProject ID PRJEB52452
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| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Springer
2023-10
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| Subjects: | pufM gene, Primer evaluation, Metagenomics, Amplicon sequencing, AAP bacteria, Conserve and sustainably use the oceans, seas and marine resources for sustainable development, |
| Online Access: | http://hdl.handle.net/10261/337803 |
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| Summary: | 12 pages, 3 figures, 1 table, supplementary information https://doi.org/10.1007/s00248-023-02220-y.-- Data Availability: Most of the analyses were performed in R version 4.2.0 (R Core Team 2022), and code is available in the following repository: https://gitlab.com/crgazulla/analysing-pufm-primers-for-marine-aap-studies. The pufM database is available in the Supplementary Material. Amplicon sequences have been deposited in the NCBI Sequence Read Archive (SRA) under BioProject ID PRJNA919028. Sequences from the BBMO metagenomic dataset were published in Auladell et al., [28], and those from the Malaspina Expedition dataset are deposited under BioProject ID PRJEB52452 |
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