Evidence for the Involvement of Aquaporins in Sperm Motility Activation of the Teleost Gilthead Sea Bream (Sparus aurata)

Evidence for the Involvement of Aquaporins in Sperm Motility Activation of the Teleost Gilthead Sea Bream (Sparus aurata)

9 pages,5 figures, 1a table

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Main Authors: Zilli, Loredana, Schiavone, Roberta, Chauvigné, François, Cerdà, Joan, Storelli, Carlo, Vilella, Sebastiano
Format: artículo biblioteca
Language:English
Italian
Published: Society for the Study of Reproduction 2009-05-01
Subjects:Aquaporins, Fish sperm, Gamete biology, Sperm, Sperm motility and transpor, Teleost,
Online Access:http://hdl.handle.net/10261/22430
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spelling dig-icm-es-10261-224302020-11-19T08:28:41Z Evidence for the Involvement of Aquaporins in Sperm Motility Activation of the Teleost Gilthead Sea Bream (Sparus aurata) Zilli, Loredana Schiavone, Roberta Chauvigné, François Cerdà, Joan Storelli, Carlo Vilella, Sebastiano Aquaporins Fish sperm Gamete biology Sperm Sperm motility and transpor Teleost 9 pages,5 figures, 1a table The expression of aquaporins in the spermatozoa of the marine teleost gilthead sea bream (Sparus aurata) and their involvement in the motility activation process were investigated. Sperm motility was activated by a hyperosmotic shock, but it was completely inhibited by 10 lM HgCl2, such inhibition being partially recovered by beta-mercaptoethanol (ME). Conventional RT-PCR using primers specific for S. aurata aquaglyceroporin (glp) and aquaporin 1a (aqp1a) demonstrated the presence of both mRNAs in spermatozoa. Heterologous expression in Xenopus laevis oocytes showed that 10 and 100 lM HgCl2 equally inhibited water and solute transport through S. aurata aquaporin 1a and S. aurata aquaglyceroporin, but treatment with ME only recovered aquaporin 1a-mediated water permeability. Western blot analysis using isoform-specific antisera on protein extracts from spermatozoa revealed bands that corresponded to the predicted molecular mass of S. aurata aquaglyceroporin (31 kDa) and S. aurata aquaporin 1a (28 kDa). The antisera also demonstrated that both aquaporins were localized in the head and flagellum of the spermatozoa. However, the immunoreaction at the plasma membrane of the spermatozoa head was more intense after the hyperosmotic activation, suggesting the translocation of both aquaporin 1a and aquaglyceroporin into the plasma membrane after the osmotic shock. This study therefore provides the first direct demonstration for the presence of aquaporins in fish sperm. The different sensitivities of S. aurata aquaporin 1a and S. aurata aquaglyceroporin to ME may explain the failure of this reducing agent to fully recover the mercurial inhibition of sperm motility, suggesting that these aquaporins may play different physiological roles during the activation and maintenance of sperm motility in sea bream. 1Supported by the Ministry of Agriculture and Aquaculture of the Region Puglia (Italy) under the program between the Region of Puglia and the University of Salento, and by Spanish Ministry of Education and Science grant AGL2007-60262/ACU to J.C. F.C. was supported by the Marie Curie Research Training Network Aqua(glycero)porins grant MRTN-CT-2006-035995 from the European Commission. Peer reviewed 2010-03-16T11:48:42Z 2010-03-16T11:48:42Z 2009-05-01 artículo http://purl.org/coar/resource_type/c_6501 Biology of Reproduction 81(5): 880–888 (2009) 0006-3363 http://hdl.handle.net/10261/22430 10.1095/biolreprod.109.077933 en it https://doi.org/10.1095/biolreprod.109.077933 none 22195 bytes application/pdf Society for the Study of Reproduction
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
Italian
topic Aquaporins
Fish sperm
Gamete biology
Sperm
Sperm motility and transpor
Teleost
Aquaporins
Fish sperm
Gamete biology
Sperm
Sperm motility and transpor
Teleost
spellingShingle Aquaporins
Fish sperm
Gamete biology
Sperm
Sperm motility and transpor
Teleost
Aquaporins
Fish sperm
Gamete biology
Sperm
Sperm motility and transpor
Teleost
Zilli, Loredana
Schiavone, Roberta
Chauvigné, François
Cerdà, Joan
Storelli, Carlo
Vilella, Sebastiano
Evidence for the Involvement of Aquaporins in Sperm Motility Activation of the Teleost Gilthead Sea Bream (Sparus aurata)
description 9 pages,5 figures, 1a table
format artículo
topic_facet Aquaporins
Fish sperm
Gamete biology
Sperm
Sperm motility and transpor
Teleost
author Zilli, Loredana
Schiavone, Roberta
Chauvigné, François
Cerdà, Joan
Storelli, Carlo
Vilella, Sebastiano
author_facet Zilli, Loredana
Schiavone, Roberta
Chauvigné, François
Cerdà, Joan
Storelli, Carlo
Vilella, Sebastiano
author_sort Zilli, Loredana
title Evidence for the Involvement of Aquaporins in Sperm Motility Activation of the Teleost Gilthead Sea Bream (Sparus aurata)
title_short Evidence for the Involvement of Aquaporins in Sperm Motility Activation of the Teleost Gilthead Sea Bream (Sparus aurata)
title_full Evidence for the Involvement of Aquaporins in Sperm Motility Activation of the Teleost Gilthead Sea Bream (Sparus aurata)
title_fullStr Evidence for the Involvement of Aquaporins in Sperm Motility Activation of the Teleost Gilthead Sea Bream (Sparus aurata)
title_full_unstemmed Evidence for the Involvement of Aquaporins in Sperm Motility Activation of the Teleost Gilthead Sea Bream (Sparus aurata)
title_sort evidence for the involvement of aquaporins in sperm motility activation of the teleost gilthead sea bream (sparus aurata)
publisher Society for the Study of Reproduction
publishDate 2009-05-01
url http://hdl.handle.net/10261/22430
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