Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata
11 pages, 8 figures
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| Language: | English |
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American Physiological Society
2008-03-01
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| Subjects: | Gilthead sea bream, SaAqp1a, SaAqp1b, Functional expression, Salinity, Gastrointestinal tract, |
| Online Access: | http://hdl.handle.net/10261/21304 |
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dig-icm-es-10261-213042020-11-19T07:24:00Z Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata Raldúa, Demetrio Otero, David Fabra, Mercedes Cerdà, Joan Gilthead sea bream SaAqp1a SaAqp1b Functional expression Salinity Gastrointestinal tract 11 pages, 8 figures Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata. Am J Physiol Regul Integr Comp Physiol 294: R993–R1003, 2008. First published January 2, 2008; doi:10.1152/ajpregu.00695.2007.—Aquaporin (AQP)-mediated intestinal water absorption may play a major osmoregulatory role in euryhaline teleosts, although the molecular identity and anatomical distribution of AQPs in the fish gastrointestinal tract is poorly known. Here, we have investigated the functional properties and cellular localization in the intestine of two gilthead seabream (Sparus aurata) homologs of mammalian aquaporin-1 (AQP1), named SaAqp1a and SaAqp1b. Heterologous expression in Xenopus laevis oocytes showed that SaAqp1a and SaAqp1b were water-selective channels. Real-time quantitative RT-PCR and Western blot using specific antisera indicated that abundance of SaAqp1a mRNA and protein was higher in duodenum and hindgut than in the rectum, whereas abundance of SaAqp1b was higher in rectum. In duodenum and hindgut, SaAqp1a localized at the apical brush border and lateral membrane of columnar enterocytes, whereas SaAqp1b was detected occasionally and at very low levels at the apical membrane. In the rectum, however, SaAqp1a was mainly accumulated in the cytoplasm of a subpopulation of enterocytes spread in groups over the surface of the epithelia, including the intervillus pockets, whereas SaAqp1b was detected exclusively at the apical brush border of all rectal enterocytes. Freshwater acclimation reduced the synthesis of SaAqp1a protein in all intestinal segments, but it only reduced SaAqp1b abundance in the rectum. These results show for the first time in teleosts a differential distribution and regulation of two functional AQP1 homologs in the intestinal epithelium, which suggest that they may play specialized functions during water movement across the intestine This work was supported by Grants from the Spanish Ministry of Education and Science (MEC; AGL2001-0364/ACU and AGL2004-00316) and the European Commission (Q5RS-2002-00784-CRYOCYTE) and by the Reference Center in Aquaculture (Generalitat de Catalunya, Spain) to J. Cerdà. Participation of M. Fabra and D. Raldúa was financed by a fellowship from the Catalan Government (DURSI, Spain) and by a “Ramón y Cajal” contract (MEC), respectively. Peer reviewed 2010-02-18T11:44:20Z 2010-02-18T11:44:20Z 2008-03-01 artículo http://purl.org/coar/resource_type/c_6501 American Journal Physiology Regulation Integrative and Comparative Physiology 294(3): 993-1003 (2008) 0363-6119 http://hdl.handle.net/10261/21304 10.1152/ajpregu.00695.2007 en https://doi.org/10.1152/ajpregu.00695.2007 none 22195 bytes application/pdf American Physiological Society |
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Gilthead sea bream SaAqp1a SaAqp1b Functional expression Salinity Gastrointestinal tract Gilthead sea bream SaAqp1a SaAqp1b Functional expression Salinity Gastrointestinal tract |
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Gilthead sea bream SaAqp1a SaAqp1b Functional expression Salinity Gastrointestinal tract Gilthead sea bream SaAqp1a SaAqp1b Functional expression Salinity Gastrointestinal tract Raldúa, Demetrio Otero, David Fabra, Mercedes Cerdà, Joan Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata |
| description |
11 pages, 8 figures |
| format |
artículo |
| topic_facet |
Gilthead sea bream SaAqp1a SaAqp1b Functional expression Salinity Gastrointestinal tract |
| author |
Raldúa, Demetrio Otero, David Fabra, Mercedes Cerdà, Joan |
| author_facet |
Raldúa, Demetrio Otero, David Fabra, Mercedes Cerdà, Joan |
| author_sort |
Raldúa, Demetrio |
| title |
Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata |
| title_short |
Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata |
| title_full |
Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata |
| title_fullStr |
Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata |
| title_full_unstemmed |
Differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost Sparus aurata |
| title_sort |
differential localization and regulation of two aquaporin-1 homologs in the intestinal epithelia of the marine teleost sparus aurata |
| publisher |
American Physiological Society |
| publishDate |
2008-03-01 |
| url |
http://hdl.handle.net/10261/21304 |
| work_keys_str_mv |
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