The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost
16 pages, 9 figures, 1 table
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Anatomical Society of Great Britain and Ireland
2018-08
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| Subjects: | Water channel, Glycerol, Aquaglyceroporin, Sperm duct, Sperm, Immunolocalization, |
| Online Access: | http://hdl.handle.net/10261/172905 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100002809 |
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dig-icm-es-10261-1729052021-12-27T16:18:44Z The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost Chauvigné, François Parhi, Janmejay Ducat, Carla Ollé, Judith Finn, Roderick N. Cerdà, Joan Generalitat de Catalunya Ministerio de Ciencia e Innovación (España) Research Council of Norway Ministry of Science and Technology (India) Water channel Glycerol Aquaglyceroporin Sperm duct Sperm Immunolocalization 16 pages, 9 figures, 1 table Aquaporin-mediated fluid transport in the mammalian efferent duct and epididymis is believed to play a role in sperm maturation and concentration. In fish, such as the marine teleost gilthead seabream (Sparus aurata), the control of fluid homeostasis in the spermatic duct seems also to be crucial for male fertility, but no information exists on the expression and distribution of aquaporins. In this study, reverse transcriptase-polymerase chain reaction and immunoblotting analyses, employing available and newly raised paralog-specific antibodies for seabream aquaporins, indicate that up to nine functional aquaporins, Aqp0a, -1aa, -1ab, -3a, -4a, -7, -8bb, -9b and -10b, are expressed in the spermatic duct. Immunolocalization of the channels in the resting spermatic duct reveals that Aqp0a, -1aa, -4a, -7 and -10b are expressed in the monolayered luminal epithelium, Aqp8b and -9b in smooth muscle fibers, and Aqp1ab and -3a in different interstitial lamina cells. In the epithelial cells, Aqp0a and -1aa are localized in the short apical microvilli, and Aqp4a and -10b show apical and basolateral staining, whereas Aqp7 is solely detected in vesicular compartments. Upon spermiation, an elongation of the epithelial cells sterocilia, as well as the folding of the epithelium, is observed. At this stage, single- and double-immunostaining, using two aquaporin paralogs or the Na/K-ATPase membrane marker, indicate that Aqp1ab, -3a, -7, -8bb and -9b staining remains unchanged, whereas in epithelial cells Aqp1aa translation is supressed, Aqp4a internalizes, and Aqp0a and -10b accumulate in the apical, lateral and basal plasma membrane. These findings uncover a cell type- and region-specific distribution of multiple aquaporins in the piscine spermatic duct, which shares conserved features of the mammalian system. The data therefore suggest that aquaporins may play different roles in the regulation of fluid homeostasis and sperm maturation in the male reproductive tract of fish This work was supported by the CERCA Programme/Government of Catalonia, and partially funded by the Spanish Ministry of Science and Innovation (Grant No. AGL2016‐76802‐R to J.C.) and the Research Council of Norway (Grant 254872/E40 to R.N.F.). The participation of J.P. was financed by an award of the Biotechnology Overseas Associateship, Indian Ministry of Science and Technology Peer Reviewed 2018-12-03T13:35:43Z 2018-12-03T13:35:43Z 2018-08 2018-12-03T13:35:45Z artículo http://purl.org/coar/resource_type/c_6501 issn: 0021-8782 e-issn: 1469-7580 Journal of Anatomy 233(2): 177-192 (2018) http://hdl.handle.net/10261/172905 10.1111/joa.12829 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100002809 29806093 https://doi.org/10.1111/joa.12829 Sí open Anatomical Society of Great Britain and Ireland |
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Water channel Glycerol Aquaglyceroporin Sperm duct Sperm Immunolocalization Water channel Glycerol Aquaglyceroporin Sperm duct Sperm Immunolocalization |
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Water channel Glycerol Aquaglyceroporin Sperm duct Sperm Immunolocalization Water channel Glycerol Aquaglyceroporin Sperm duct Sperm Immunolocalization Chauvigné, François Parhi, Janmejay Ducat, Carla Ollé, Judith Finn, Roderick N. Cerdà, Joan The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost |
| description |
16 pages, 9 figures, 1 table |
| author2 |
Generalitat de Catalunya |
| author_facet |
Generalitat de Catalunya Chauvigné, François Parhi, Janmejay Ducat, Carla Ollé, Judith Finn, Roderick N. Cerdà, Joan |
| format |
artículo |
| topic_facet |
Water channel Glycerol Aquaglyceroporin Sperm duct Sperm Immunolocalization |
| author |
Chauvigné, François Parhi, Janmejay Ducat, Carla Ollé, Judith Finn, Roderick N. Cerdà, Joan |
| author_sort |
Chauvigné, François |
| title |
The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost |
| title_short |
The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost |
| title_full |
The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost |
| title_fullStr |
The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost |
| title_full_unstemmed |
The cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost |
| title_sort |
cellular localization and redistribution of multiple aquaporin paralogs in the spermatic duct epithelium of a maturing marine teleost |
| publisher |
Anatomical Society of Great Britain and Ireland |
| publishDate |
2018-08 |
| url |
http://hdl.handle.net/10261/172905 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100002809 |
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| _version_ |
1777666832976576512 |