Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules of Medicago truncatula: Two Ugly Items That Hinder Coping with Salt Stress Effects
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Multidisciplinary Digital Publishing Institute
2022-09-02
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| Subjects: | Symbiosis, Ion transport, Salt stress, Sodium, Potassium, NHX Na+/K+ exchangers, Medicago truncatula, Root nodule, |
| Online Access: | http://hdl.handle.net/10261/356388 http://dx.doi.org/10.13039/501100002261 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 |
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Symbiosis Ion transport Salt stress Sodium Potassium NHX Na+/K+ exchangers Medicago truncatula Root nodule Symbiosis Ion transport Salt stress Sodium Potassium NHX Na+/K+ exchangers Medicago truncatula Root nodule |
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Symbiosis Ion transport Salt stress Sodium Potassium NHX Na+/K+ exchangers Medicago truncatula Root nodule Symbiosis Ion transport Salt stress Sodium Potassium NHX Na+/K+ exchangers Medicago truncatula Root nodule Trifonova, Natalia A. Kamyshinsky, Roman Coba de la Peña, Teodoro Koroleva, Maria I. Kulikova, Olga Lara-Dampier, Victoria Pashkovskiy, Pavel Presniakov, Mikhail Pueyo, José Javier Lucas, M. Mercedes Elena E. Fedorova Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules of Medicago truncatula: Two Ugly Items That Hinder Coping with Salt Stress Effects |
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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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Russian Foundation for Basic Research |
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Russian Foundation for Basic Research Trifonova, Natalia A. Kamyshinsky, Roman Coba de la Peña, Teodoro Koroleva, Maria I. Kulikova, Olga Lara-Dampier, Victoria Pashkovskiy, Pavel Presniakov, Mikhail Pueyo, José Javier Lucas, M. Mercedes Elena E. Fedorova |
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artículo |
| topic_facet |
Symbiosis Ion transport Salt stress Sodium Potassium NHX Na+/K+ exchangers Medicago truncatula Root nodule |
| author |
Trifonova, Natalia A. Kamyshinsky, Roman Coba de la Peña, Teodoro Koroleva, Maria I. Kulikova, Olga Lara-Dampier, Victoria Pashkovskiy, Pavel Presniakov, Mikhail Pueyo, José Javier Lucas, M. Mercedes Elena E. Fedorova |
| author_sort |
Trifonova, Natalia A. |
| title |
Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules of Medicago truncatula: Two Ugly Items That Hinder Coping with Salt Stress Effects |
| title_short |
Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules of Medicago truncatula: Two Ugly Items That Hinder Coping with Salt Stress Effects |
| title_full |
Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules of Medicago truncatula: Two Ugly Items That Hinder Coping with Salt Stress Effects |
| title_fullStr |
Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules of Medicago truncatula: Two Ugly Items That Hinder Coping with Salt Stress Effects |
| title_full_unstemmed |
Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules of Medicago truncatula: Two Ugly Items That Hinder Coping with Salt Stress Effects |
| title_sort |
sodium accumulation in infected cells and ion transporters mistargeting in nodules of medicago truncatula: two ugly items that hinder coping with salt stress effects |
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Multidisciplinary Digital Publishing Institute |
| publishDate |
2022-09-02 |
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http://hdl.handle.net/10261/356388 http://dx.doi.org/10.13039/501100002261 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 |
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dig-ica-es-10261-3563882024-05-08T15:55:40Z Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules of Medicago truncatula: Two Ugly Items That Hinder Coping with Salt Stress Effects Trifonova, Natalia A. Kamyshinsky, Roman Coba de la Peña, Teodoro Koroleva, Maria I. Kulikova, Olga Lara-Dampier, Victoria Pashkovskiy, Pavel Presniakov, Mikhail Pueyo, José Javier Lucas, M. Mercedes Elena E. Fedorova Russian Foundation for Basic Research Ministry of Science and Higher Education of the Russian Federation Comunidad de Madrid Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) European Commission Ministerio de Educación (España) Coba de la Peña, Teodoro [0000-0002-6354-297X] Pueyo, José Javier [0000-0003-0337-4078] Lucas, M. Mercedes [0000-0002-3252-0145] Symbiosis Ion transport Salt stress Sodium Potassium NHX Na+/K+ exchangers Medicago truncatula Root nodule © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). The maintenance of intracellular nitrogen-fixing bacteria causes changes in proteins’ location and in gene expression that may be detrimental to the host cell fitness. We hypothesized that the nodule’s high vulnerability toward salt stress might be due to alterations in mechanisms involved in the exclusion of Na+ from the host cytoplasm. Confocal and electron microscopy immunolocalization analyses of Na+/K+ exchangers in the root nodule showed the plasma membrane (MtNHX7) and endosome/tonoplast (MtNHX6) signal in non-infected cells; however, in mature infected cells the proteins were depleted from their target membranes and expelled to vacuoles. This mistargeting suggests partial loss of the exchanger’s functionality in these cells. In the mature part of the nodule 7 of the 20 genes encoding ion transporters, channels, and Na+/K+ exchangers were either not expressed or substantially downregulated. In nodules from plants subjected to salt treatments, low temperature-scanning electron microscopy and X-ray microanalysis revealed the accumulation of 5–6 times more Na+ per infected cell versus non-infected one. Hence, the infected cells’ inability to withstand the salt may be the integral result of preexisting defects in the localization of proteins involved in Na+ exclusion and the reduced expression of key genes of ion homeostasis, resulting in premature senescence and termination of symbiosis. The work was supported by the RFBR (Russian Foundation for Basic Research, grant 19-04-00570 to EEF), and the results of IDA were obtained by funding within the state assignment of Ministry of Science and Higher Education of the Russian Federation (theme No. АААА-А19-119041690042-7 and theme No. 122042700044-6), Comunidad de Madrid (grant S2009/AMB-151 to MML), AEI/FEDER-UE (grants AGL2017-88381-R and PID2021-11253710B-100 to JJP and MML), and the Spanish Ministry of Education (sabbatical year contract ref. SAB2010-0086 to EEF). Peer reviewed 2024-05-08T15:55:39Z 2024-05-08T15:55:39Z 2022-09-02 artículo http://purl.org/coar/resource_type/c_6501 International Journal of Molecular Sciences 23(18): 10618 (2022) http://hdl.handle.net/10261/356388 10.3390/ijms231810618 1422-0067 http://dx.doi.org/10.13039/501100002261 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# S2009/AMB-151 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88381-R/ES/RESPUESTA DE LA SIMBIOSIS RIZOBIO-LEGUMINOSA A METALES PESADOS: DE ANALISIS OMICOS A LA FITORREMEDIACION DE SUELOS CONTAMINADOS/ info:eu-repo/grantAgreement/AEI//PID2021-11253710B-I00 info:eu-repo/grantAgreement/ME//SAB2010-0086/ES/SAB2010-0086/ Publisher's version The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.3390/ijms231810618 https://doi.org/10.3390/ijms231810618 Sí open application/pdf Multidisciplinary Digital Publishing Institute |