Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet
The interaction between diet and intestinal health has been widely discussed, although in vivo approaches have reported limitations. The intestine explant culture system developed provides an advantage since it reduces the number of experimental fish and increases the time of incubation compared to similar methods, becoming a valuable tool in the study of the interactions between pathogenic bacteria, rearing conditions, or dietary components and fish gut immune response. The objective of this study was to determine the influence of the total substitution of fish meal by plants on the immune intestinal status of seabream using an ex vivo bacterial challenge. For this aim, two growth stages of fish were assayed (12 g): phase I (90 days), up to 68 g, and phase II (305 days), up to 250 g. Additionally, in phase II, the effects of long term and short term exposure (15 days) to a plant protein (PP) diet were determined. PP diet altered the mucosal immune homeostasis, the younger fish being more sensitive, and the intestine from fish fed short-term plant diets showed a higher immune response than with long-term feeding. Vibrio alginolyticus (V. alginolyticus) triggered the highest immune and inflammatory response, while COX-2 expression was significantly induced by Photobacterium damselae subsp. Piscicida (P. damselae subsp. Piscicida), showing a positive high correlation between the pro-inflammatory genes encoding interleukin 1β (IL1-β), interleukin 6 (IL-6) and cyclooxygenase 2(COX-2).
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Language: | English |
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Multidisciplinary Digital Publishing Institute
2020-10-14
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Subjects: | Gilthead seabream, ex vivo, Intestine explants culture, RT-qPCR, Inflammation, Plant protein, |
Online Access: | http://hdl.handle.net/10261/239154 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 |
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dig-iata-es-10261-2391542021-12-27T15:38:16Z Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet Sánchez Peñaranda, David Bäuerl, Christine Tomás Vidal, Ana Jover Cerdá, Miguel Estruch, Guillem Pérez Martínez, Gaspar Martínez Llorens, Silvia Ministerio de Economía y Competitividad (España) Generalitat Valenciana Gilthead seabream ex vivo Intestine explants culture RT-qPCR Inflammation Plant protein The interaction between diet and intestinal health has been widely discussed, although in vivo approaches have reported limitations. The intestine explant culture system developed provides an advantage since it reduces the number of experimental fish and increases the time of incubation compared to similar methods, becoming a valuable tool in the study of the interactions between pathogenic bacteria, rearing conditions, or dietary components and fish gut immune response. The objective of this study was to determine the influence of the total substitution of fish meal by plants on the immune intestinal status of seabream using an ex vivo bacterial challenge. For this aim, two growth stages of fish were assayed (12 g): phase I (90 days), up to 68 g, and phase II (305 days), up to 250 g. Additionally, in phase II, the effects of long term and short term exposure (15 days) to a plant protein (PP) diet were determined. PP diet altered the mucosal immune homeostasis, the younger fish being more sensitive, and the intestine from fish fed short-term plant diets showed a higher immune response than with long-term feeding. Vibrio alginolyticus (V. alginolyticus) triggered the highest immune and inflammatory response, while COX-2 expression was significantly induced by Photobacterium damselae subsp. Piscicida (P. damselae subsp. Piscicida), showing a positive high correlation between the pro-inflammatory genes encoding interleukin 1β (IL1-β), interleukin 6 (IL-6) and cyclooxygenase 2(COX-2). The research was supported by a grant financed by the Spanish Ministerio de Economía y Competitividad AGL2015-70487-P and Generalitat Valenciana, IDIFEDER/2020/029 Peer reviewed 2021-04-22T18:07:31Z 2021-04-22T18:07:31Z 2020-10-14 artículo http://purl.org/coar/resource_type/c_6501 International Journal of Molecular Sciences 1(20): 7584 (2020) http://hdl.handle.net/10261/239154 10.3390/ijms21207584 1422-0067 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 33066515 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-70487-P Publisher's version https://doi.org/10.3390/ijms21207584 Sí open Multidisciplinary Digital Publishing Institute |
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Gilthead seabream ex vivo Intestine explants culture RT-qPCR Inflammation Plant protein Gilthead seabream ex vivo Intestine explants culture RT-qPCR Inflammation Plant protein |
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Gilthead seabream ex vivo Intestine explants culture RT-qPCR Inflammation Plant protein Gilthead seabream ex vivo Intestine explants culture RT-qPCR Inflammation Plant protein Sánchez Peñaranda, David Bäuerl, Christine Tomás Vidal, Ana Jover Cerdá, Miguel Estruch, Guillem Pérez Martínez, Gaspar Martínez Llorens, Silvia Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet |
description |
The interaction between diet and intestinal health has been widely discussed, although in vivo approaches have reported limitations. The intestine explant culture system developed provides an advantage since it reduces the number of experimental fish and increases the time of incubation compared to similar methods, becoming a valuable tool in the study of the interactions between pathogenic bacteria, rearing conditions, or dietary components and fish gut immune response. The objective of this study was to determine the influence of the total substitution of fish meal by plants on the immune intestinal status of seabream using an ex vivo bacterial challenge. For this aim, two growth stages of fish were assayed (12 g): phase I (90 days), up to 68 g, and phase II (305 days), up to 250 g. Additionally, in phase II, the effects of long term and short term exposure (15 days) to a plant protein (PP) diet were determined. PP diet altered the mucosal immune homeostasis, the younger fish being more sensitive, and the intestine from fish fed short-term plant diets showed a higher immune response than with long-term feeding. Vibrio alginolyticus (V. alginolyticus) triggered the highest immune and inflammatory response, while COX-2 expression was significantly induced by Photobacterium damselae subsp. Piscicida (P. damselae subsp. Piscicida), showing a positive high correlation between the pro-inflammatory genes encoding interleukin 1β (IL1-β), interleukin 6 (IL-6) and cyclooxygenase 2(COX-2). |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Sánchez Peñaranda, David Bäuerl, Christine Tomás Vidal, Ana Jover Cerdá, Miguel Estruch, Guillem Pérez Martínez, Gaspar Martínez Llorens, Silvia |
format |
artículo |
topic_facet |
Gilthead seabream ex vivo Intestine explants culture RT-qPCR Inflammation Plant protein |
author |
Sánchez Peñaranda, David Bäuerl, Christine Tomás Vidal, Ana Jover Cerdá, Miguel Estruch, Guillem Pérez Martínez, Gaspar Martínez Llorens, Silvia |
author_sort |
Sánchez Peñaranda, David |
title |
Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet |
title_short |
Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet |
title_full |
Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet |
title_fullStr |
Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet |
title_full_unstemmed |
Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet |
title_sort |
intestinal explant cultures from gilthead seabream (sparus aurata, l.) allowed the determination of mucosal sensitivity to bacterial pathogens and the impact of a plant protein diet |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020-10-14 |
url |
http://hdl.handle.net/10261/239154 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 |
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