Germination characteristics and storage behavior of Codonopsis pilosula Nannf. seeds
<i>Codonopsis pilosula</i> Nannf. is classified as an endangered species (VU) in Korea. Ex situ conservation through long-term storage of seeds is, in principle, possible for a significant proportion of plants. However, it is not known whether these seeds are orthodox; that is, if they can survive under long-term storage conditions (-20 °C, 40 % relative humidity (RH) or less) without being damaged. We determined the germination characteristics and storage behavior of C. pilosula. The seeds were collected from plants growing at the Baekdudaegan Arboretum on September 17, 2020. Immediately after collection, the equilibrium relative humidity (eRH) of the seeds was measured. To determine the germination characteristics of the seeds, a germination test was conducted at temperatures of 15, 20, and 25 °C (12/12 h) with 100 and 250 ppm GA3. To determine the storage behavior, their moisture content was measured under five conditions: 15 °C and RH at 15, 20, 30, 40, and 50 %, at 250 ppm GA3. Vitality was calculated as the ratio of the number of germinated seeds and the survived seeds of the unsprouted through the TZ test. Viability tests were conducted in the order of X-ray, germination test, and TZ test. The average germination percentage (GP) of untreated seeds was below 11 ± 3 %. The highest GP of seeds following GA3 treatment was 90 ± 0.70 %. The eRH was 45 % after collection. The initial seed viability percentage was 90.3 ± 2 %. The highest viability percentage (VP) was identified at 91 ± 0.25 % in equilibrium with 20 % (eRH) at 15 °C with a moisture content of 3.23 % Fresh weight. The results of our study on the behavior of C. pilosula seeds indicate that orthodox seeds did not reduce in viability even when dried in equilibrium with 20 % RH; thus, these seeds can be kept under long-term storage conditions. Keywords: Biodiversity conservation, Climate change, Agriculture ID: 3622450
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| Format: | Document biblioteca |
| Language: | English |
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FAO ;
2022
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| Online Access: | https://openknowledge.fao.org/handle/20.500.14283/CC2638EN http://www.fao.org/3/cc2638en/cc2638en.pdf |
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| Summary: | <i>Codonopsis pilosula</i> Nannf. is classified as an endangered species (VU) in Korea. Ex situ conservation through long-term storage of seeds is, in principle, possible for a significant proportion of plants. However, it is not known whether these seeds are orthodox; that is, if they can survive under long-term storage conditions (-20 °C, 40 % relative humidity (RH) or less) without being damaged. We determined the germination characteristics and storage behavior of C. pilosula. The seeds were collected from plants growing at the Baekdudaegan Arboretum on September 17, 2020. Immediately after collection, the equilibrium relative humidity (eRH) of the seeds was measured. To determine the germination characteristics of the seeds, a germination test was conducted at temperatures of 15, 20, and 25 °C (12/12 h) with 100 and 250 ppm GA3. To determine the storage behavior, their moisture content was measured under five conditions: 15 °C and RH at 15, 20, 30, 40, and 50 %, at 250 ppm GA3. Vitality was calculated as the ratio of the number of germinated seeds and the survived seeds of the unsprouted through the TZ test. Viability tests were conducted in the order of X-ray, germination test, and TZ test. The average germination percentage (GP) of untreated seeds was below 11 ± 3 %. The highest GP of seeds following GA3 treatment was 90 ± 0.70 %. The eRH was 45 % after collection. The initial seed viability percentage was 90.3 ± 2 %. The highest viability percentage (VP) was identified at 91 ± 0.25 % in equilibrium with 20 % (eRH) at 15 °C with a moisture content of 3.23 % Fresh weight. The results of our study on the behavior of C. pilosula seeds indicate that orthodox seeds did not reduce in viability even when dried in equilibrium with 20 % RH; thus, these seeds can be kept under long-term storage conditions.
Keywords: Biodiversity conservation, Climate change, Agriculture
ID: 3622450 |
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