Genomic approach to study floral development genes in Rosa sp.
Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and flowers to establish a precise morphological calendar from early rose flower development stages to senescing flowers. Global gene expression was investigated from floral meristem initiation up to flower senescence in three rose genotypes exhibiting contrasted floral traits including continuous versus once flowering and simple versus double flower architecture, using a newly developed Affymetrix microarray (Rosa1_Affyarray) tool containing sequences representing 4765 unigenes expressed during flower development. Data analyses permitted the identification of genes associated with floral transition, floral organs initiation up to flower senescence. Quantitative real time PCR analyses validated the mRNA accumulation changes observed in microarray hybridizations for a selection of 24 genes expressed at either high or low levels. Our data describe the early flower development stages in Rosa sp, the production of a rose microarray and demonstrate its usefulness and reliability to study gene expression during extensive development phases, from the vegetative meristem to the senescent flower.
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Subjects: | F30 - Génétique et amélioration des plantes, F62 - Physiologie végétale - Croissance et développement, phytogénétique, génomique, expression des gènes, stade de développement végétal, floraison, méristème, Rosa, http://aims.fao.org/aos/agrovoc/c_49985, http://aims.fao.org/aos/agrovoc/c_92382, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_5959, http://aims.fao.org/aos/agrovoc/c_2992, http://aims.fao.org/aos/agrovoc/c_4754, http://aims.fao.org/aos/agrovoc/c_6655, |
Online Access: | http://agritrop.cirad.fr/600453/ http://agritrop.cirad.fr/600453/1/Dubois_et_al_2011_Plos_One.pdf |
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dig-cirad-fr-6004532024-01-29T04:03:04Z http://agritrop.cirad.fr/600453/ http://agritrop.cirad.fr/600453/ Genomic approach to study floral development genes in Rosa sp. Dubois Annick, Remay Arnaud, Raymond Olivier, Balzergue Sandrine, Chauvet Aurélie, Maene Marion, Pecrix Yann, Yang Shu-Hua, Jeauffre julien, Thouroude Tatiana, Boltz Véronique, Martin-Magniette Marie-Laure, Janczarski Stéphane, Legeai Fabrice, Renou Jean-Pierre, Vergne Philippe, Le Bris Manuel, Foucher Fabrice, Bendahmane Mohammed. 2011. PloS One, 6 (12):e28455, 14 p.https://doi.org/10.1371/journal.pone.0028455 <https://doi.org/10.1371/journal.pone.0028455> Genomic approach to study floral development genes in Rosa sp. Dubois, Annick Remay, Arnaud Raymond, Olivier Balzergue, Sandrine Chauvet, Aurélie Maene, Marion Pecrix, Yann Yang, Shu-Hua Jeauffre, julien Thouroude, Tatiana Boltz, Véronique Martin-Magniette, Marie-Laure Janczarski, Stéphane Legeai, Fabrice Renou, Jean-Pierre Vergne, Philippe Le Bris, Manuel Foucher, Fabrice Bendahmane, Mohammed eng 2011 PloS One F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement phytogénétique génomique expression des gènes stade de développement végétal floraison méristème Rosa http://aims.fao.org/aos/agrovoc/c_49985 http://aims.fao.org/aos/agrovoc/c_92382 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_2992 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_6655 Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and flowers to establish a precise morphological calendar from early rose flower development stages to senescing flowers. Global gene expression was investigated from floral meristem initiation up to flower senescence in three rose genotypes exhibiting contrasted floral traits including continuous versus once flowering and simple versus double flower architecture, using a newly developed Affymetrix microarray (Rosa1_Affyarray) tool containing sequences representing 4765 unigenes expressed during flower development. Data analyses permitted the identification of genes associated with floral transition, floral organs initiation up to flower senescence. Quantitative real time PCR analyses validated the mRNA accumulation changes observed in microarray hybridizations for a selection of 24 genes expressed at either high or low levels. Our data describe the early flower development stages in Rosa sp, the production of a rose microarray and demonstrate its usefulness and reliability to study gene expression during extensive development phases, from the vegetative meristem to the senescent flower. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/600453/1/Dubois_et_al_2011_Plos_One.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1371/journal.pone.0028455 10.1371/journal.pone.0028455 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0028455 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1371/journal.pone.0028455 |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement phytogénétique génomique expression des gènes stade de développement végétal floraison méristème Rosa http://aims.fao.org/aos/agrovoc/c_49985 http://aims.fao.org/aos/agrovoc/c_92382 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_2992 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_6655 F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement phytogénétique génomique expression des gènes stade de développement végétal floraison méristème Rosa http://aims.fao.org/aos/agrovoc/c_49985 http://aims.fao.org/aos/agrovoc/c_92382 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_2992 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_6655 |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement phytogénétique génomique expression des gènes stade de développement végétal floraison méristème Rosa http://aims.fao.org/aos/agrovoc/c_49985 http://aims.fao.org/aos/agrovoc/c_92382 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_2992 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_6655 F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement phytogénétique génomique expression des gènes stade de développement végétal floraison méristème Rosa http://aims.fao.org/aos/agrovoc/c_49985 http://aims.fao.org/aos/agrovoc/c_92382 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_2992 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_6655 Dubois, Annick Remay, Arnaud Raymond, Olivier Balzergue, Sandrine Chauvet, Aurélie Maene, Marion Pecrix, Yann Yang, Shu-Hua Jeauffre, julien Thouroude, Tatiana Boltz, Véronique Martin-Magniette, Marie-Laure Janczarski, Stéphane Legeai, Fabrice Renou, Jean-Pierre Vergne, Philippe Le Bris, Manuel Foucher, Fabrice Bendahmane, Mohammed Genomic approach to study floral development genes in Rosa sp. |
description |
Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and flowers to establish a precise morphological calendar from early rose flower development stages to senescing flowers. Global gene expression was investigated from floral meristem initiation up to flower senescence in three rose genotypes exhibiting contrasted floral traits including continuous versus once flowering and simple versus double flower architecture, using a newly developed Affymetrix microarray (Rosa1_Affyarray) tool containing sequences representing 4765 unigenes expressed during flower development. Data analyses permitted the identification of genes associated with floral transition, floral organs initiation up to flower senescence. Quantitative real time PCR analyses validated the mRNA accumulation changes observed in microarray hybridizations for a selection of 24 genes expressed at either high or low levels. Our data describe the early flower development stages in Rosa sp, the production of a rose microarray and demonstrate its usefulness and reliability to study gene expression during extensive development phases, from the vegetative meristem to the senescent flower. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement phytogénétique génomique expression des gènes stade de développement végétal floraison méristème Rosa http://aims.fao.org/aos/agrovoc/c_49985 http://aims.fao.org/aos/agrovoc/c_92382 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5959 http://aims.fao.org/aos/agrovoc/c_2992 http://aims.fao.org/aos/agrovoc/c_4754 http://aims.fao.org/aos/agrovoc/c_6655 |
author |
Dubois, Annick Remay, Arnaud Raymond, Olivier Balzergue, Sandrine Chauvet, Aurélie Maene, Marion Pecrix, Yann Yang, Shu-Hua Jeauffre, julien Thouroude, Tatiana Boltz, Véronique Martin-Magniette, Marie-Laure Janczarski, Stéphane Legeai, Fabrice Renou, Jean-Pierre Vergne, Philippe Le Bris, Manuel Foucher, Fabrice Bendahmane, Mohammed |
author_facet |
Dubois, Annick Remay, Arnaud Raymond, Olivier Balzergue, Sandrine Chauvet, Aurélie Maene, Marion Pecrix, Yann Yang, Shu-Hua Jeauffre, julien Thouroude, Tatiana Boltz, Véronique Martin-Magniette, Marie-Laure Janczarski, Stéphane Legeai, Fabrice Renou, Jean-Pierre Vergne, Philippe Le Bris, Manuel Foucher, Fabrice Bendahmane, Mohammed |
author_sort |
Dubois, Annick |
title |
Genomic approach to study floral development genes in Rosa sp. |
title_short |
Genomic approach to study floral development genes in Rosa sp. |
title_full |
Genomic approach to study floral development genes in Rosa sp. |
title_fullStr |
Genomic approach to study floral development genes in Rosa sp. |
title_full_unstemmed |
Genomic approach to study floral development genes in Rosa sp. |
title_sort |
genomic approach to study floral development genes in rosa sp. |
url |
http://agritrop.cirad.fr/600453/ http://agritrop.cirad.fr/600453/1/Dubois_et_al_2011_Plos_One.pdf |
work_keys_str_mv |
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_version_ |
1792500317059809280 |