High‑throughput sequencing of complete genomes of ipomoviruses associated with an epidemic of cassava brown streak disease in the Comoros Archipelago

Using high-throughput sequencing of small interfering RNAs (siRNAs), virion-associated nucleic acid (VANA), and double stranded RNAs (dsRNAs), we have determined the complete genome sequences of Comorian isolates of two ipomoviruses, cassava brown streak virus (CBSV) and a divergent isolate of Ugandan cassava brown streak virus (UCBSV-KM) representing a new strain of this virus. While the large ORF of CBSV shares the highest nucleotide sequence identity (95.9%) with a Tanzanian isolate of CBSV, the large UCBSV-KM ORF shares the highest nucleotide sequence identity (77.5%) with a Malawian isolate of UCBSV. This low value is near the species demarcation threshold for the family Potyviridae (<76%). Phylogenetic analysis confirms that UCBSV-KM represents a new lineage that is genetically distinct from the currently described UCBSV strains.

Bibliographic Details

Main Authors:	Scussel, Sarah, Candresse, Thierry, Marais, Armelle, Claverie, Sohini, Hoareau, Murielle, Azali Hamza, Abdou, Verdin, Eric, Tepfer, Mark, Filloux, Denis, Fernandez, Emmanuel, Roumagnac, Philippe, Robene, Isabelle, Lefeuvre, Pierre, Jourda, Cyril, Roux-Cuvelier, Michel, Lett, Jean-Michel
Format:	article biblioteca
Language:	eng
Subjects:	H20 - Maladies des plantes, manioc, Potyviridae, génome, séquence nucléotidique, http://aims.fao.org/aos/agrovoc/c_9649, http://aims.fao.org/aos/agrovoc/c_3379026b, http://aims.fao.org/aos/agrovoc/c_3224, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_1790,
Online Access:	http://agritrop.cirad.fr/592647/ http://agritrop.cirad.fr/592647/7/Scussel2019_Article_High-throughputSequencingOfCom.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!