First step of ABA-mediated response to drought in Coffea
Drought and high temperatures are key factors which affect coffee plant development and production. Coffea canephora drought tolerant clones (Cc14, Cc73 and Cc120) have been characterized as vigorous plants with high productivity throughout years under water deficit. Recently, a new mechanism of ABA transduction was proposed, involving PYR/PYL/RCARs receptors interacting with PP2Cs phosphatases and SnRK2 protein kinases. The goal of this study was to identify and characterize orthologous genes of this tripartite system in coffee. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, and tomato were chosen as query to search orthologous genes in sequence databases. This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCAR, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. Phylogenetic analyses allowed to classify coffee polypeptides sequences between subclasses and subfamilies expected. The gene structures of these three gene families were functionally annotated in the reference genome (Coffee Genome Hub - http://coffee-genome.org/). Tissue (leaf, seed, root and floral organ) differential expression was verified through in silico analyses. Regarding drought conditions, data from root transcriptome showed contrasting gene expression for those genes between the tolerant (Cc14, Cc73 and Cc120) and susceptible (Cc22) clones. Moreover, differential responses were also observed comparing gene expression profiles among drought tolerant clones under water deficit which suggest the existence of multiple biological mechanisms for drought tolerance in coffee. All those evidences will help to identify the genetic determinism of drought tolerance essential to obtain molecular markers that could be used in coffee breeding programs. (Texte intégral)
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Subjects: | F62 - Physiologie végétale - Croissance et développement, F30 - Génétique et amélioration des plantes, H50 - Troubles divers des plantes, P40 - Météorologie et climatologie, |
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dig-cirad-fr-5782902019-12-01T17:02:27Z http://agritrop.cirad.fr/578290/ http://agritrop.cirad.fr/578290/ First step of ABA-mediated response to drought in Coffea. Cotta Michelle G., Marraccini Pierre, Rego Erica C.S., Sidibé-Bocs Stéphanie, Carneiro Fernanda A, This Dominique, Andrade Alan Carvalho. 2015. . Iguaçu Falls : ISPMB, Résumé, 1 p. International Congress of Plant Molecular Biology. 11, Iguaçu Falls, Brésil, 25 Octobre 2015/30 Octobre 2015.http://www.ipmb2015.org/Overview.php <http://www.ipmb2015.org/Overview.php> Researchers First step of ABA-mediated response to drought in Coffea Cotta, Michelle G. Marraccini, Pierre Rego, Erica C.S. Sidibé-Bocs, Stéphanie Carneiro, Fernanda A This, Dominique Andrade, Alan Carvalho eng 2015 ISPMB F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes P40 - Météorologie et climatologie Drought and high temperatures are key factors which affect coffee plant development and production. Coffea canephora drought tolerant clones (Cc14, Cc73 and Cc120) have been characterized as vigorous plants with high productivity throughout years under water deficit. Recently, a new mechanism of ABA transduction was proposed, involving PYR/PYL/RCARs receptors interacting with PP2Cs phosphatases and SnRK2 protein kinases. The goal of this study was to identify and characterize orthologous genes of this tripartite system in coffee. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, and tomato were chosen as query to search orthologous genes in sequence databases. This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCAR, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. Phylogenetic analyses allowed to classify coffee polypeptides sequences between subclasses and subfamilies expected. The gene structures of these three gene families were functionally annotated in the reference genome (Coffee Genome Hub - http://coffee-genome.org/). Tissue (leaf, seed, root and floral organ) differential expression was verified through in silico analyses. Regarding drought conditions, data from root transcriptome showed contrasting gene expression for those genes between the tolerant (Cc14, Cc73 and Cc120) and susceptible (Cc22) clones. Moreover, differential responses were also observed comparing gene expression profiles among drought tolerant clones under water deficit which suggest the existence of multiple biological mechanisms for drought tolerance in coffee. All those evidences will help to identify the genetic determinism of drought tolerance essential to obtain molecular markers that could be used in coffee breeding programs. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/578290/1/854.pdf text cc_by_nd info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nd/4.0/ http://www.ipmb2015.org/Overview.php info:eu-repo/semantics/altIdentifier/purl/http://www.ipmb2015.org/Overview.php |
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F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes P40 - Météorologie et climatologie F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes P40 - Météorologie et climatologie |
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F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes P40 - Météorologie et climatologie F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes P40 - Météorologie et climatologie Cotta, Michelle G. Marraccini, Pierre Rego, Erica C.S. Sidibé-Bocs, Stéphanie Carneiro, Fernanda A This, Dominique Andrade, Alan Carvalho First step of ABA-mediated response to drought in Coffea |
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Drought and high temperatures are key factors which affect coffee plant development and production. Coffea canephora drought tolerant clones (Cc14, Cc73 and Cc120) have been characterized as vigorous plants with high productivity throughout years under water deficit. Recently, a new mechanism of ABA transduction was proposed, involving PYR/PYL/RCARs receptors interacting with PP2Cs phosphatases and SnRK2 protein kinases. The goal of this study was to identify and characterize orthologous genes of this tripartite system in coffee. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, and tomato were chosen as query to search orthologous genes in sequence databases. This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCAR, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. Phylogenetic analyses allowed to classify coffee polypeptides sequences between subclasses and subfamilies expected. The gene structures of these three gene families were functionally annotated in the reference genome (Coffee Genome Hub - http://coffee-genome.org/). Tissue (leaf, seed, root and floral organ) differential expression was verified through in silico analyses. Regarding drought conditions, data from root transcriptome showed contrasting gene expression for those genes between the tolerant (Cc14, Cc73 and Cc120) and susceptible (Cc22) clones. Moreover, differential responses were also observed comparing gene expression profiles among drought tolerant clones under water deficit which suggest the existence of multiple biological mechanisms for drought tolerance in coffee. All those evidences will help to identify the genetic determinism of drought tolerance essential to obtain molecular markers that could be used in coffee breeding programs. (Texte intégral) |
format |
conference_item |
topic_facet |
F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes P40 - Météorologie et climatologie |
author |
Cotta, Michelle G. Marraccini, Pierre Rego, Erica C.S. Sidibé-Bocs, Stéphanie Carneiro, Fernanda A This, Dominique Andrade, Alan Carvalho |
author_facet |
Cotta, Michelle G. Marraccini, Pierre Rego, Erica C.S. Sidibé-Bocs, Stéphanie Carneiro, Fernanda A This, Dominique Andrade, Alan Carvalho |
author_sort |
Cotta, Michelle G. |
title |
First step of ABA-mediated response to drought in Coffea |
title_short |
First step of ABA-mediated response to drought in Coffea |
title_full |
First step of ABA-mediated response to drought in Coffea |
title_fullStr |
First step of ABA-mediated response to drought in Coffea |
title_full_unstemmed |
First step of ABA-mediated response to drought in Coffea |
title_sort |
first step of aba-mediated response to drought in coffea |
publisher |
ISPMB |
url |
http://agritrop.cirad.fr/578290/ http://agritrop.cirad.fr/578290/1/854.pdf |
work_keys_str_mv |
AT cottamichelleg firststepofabamediatedresponsetodroughtincoffea AT marraccinipierre firststepofabamediatedresponsetodroughtincoffea AT regoericacs firststepofabamediatedresponsetodroughtincoffea AT sidibebocsstephanie firststepofabamediatedresponsetodroughtincoffea AT carneirofernandaa firststepofabamediatedresponsetodroughtincoffea AT thisdominique firststepofabamediatedresponsetodroughtincoffea AT andradealancarvalho firststepofabamediatedresponsetodroughtincoffea |
_version_ |
1758024732213510144 |