Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidisin wild Asian amphibians
Population declines and extinctions of amphibians have been attributed to the chytrid fungus Batrachochytrium dendrobatidis (Bd), especially one globally emerging recombinant lineage ('Bd-GPL'). We used PCR assays that target the ribosomal internal transcribed spacer region (ITS) of Bd to determine the prevalence and genetic diversity of Bd in South Korea, where Bd is widely distributed but is not known to cause morbidity or mortality in wild populations. We isolated Korean Bd strains from native amphibians with low infection loads and compared them to known worldwide Bd strains using 19 polymorphic SNP and microsatellite loci. Bd prevalence ranged between 12.5 and 48.0%, in 11 of 17 native Korean species, and 24.7% in the introduced bullfrog Lithobates catesbeianus. Based on ITS sequence variation, 47 of the 50 identified Korean haplotypes formed a group closely associated with a native Brazilian Bd lineage, separated from the Bd-GPL lineage. However, multilocus genotyping of three Korean Bd isolates revealed strong divergence from both Bd-GPL and the native Brazilian Bd lineages. Thus, the ITS region resolves genotypes that diverge from Bd-GPL but otherwise generates ambiguous phylogenies. Our results point to the presence of highly diversified endemic strains of Bd across Asian amphibian species. The rarity of Bd-GPL-associated haplotypes suggests that either this lineage was introduced into Korea only recently or Bd-GPL has been outcompeted by native Bd strains. Our results highlight the need to consider possible complex interactions among native Bd lineages, Bd-GPL and their associated amphibian hosts when assessing the spread and impact of Bd-GPL on worldwide amphibian populations.
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dig-cirad-fr-5782212022-04-15T12:55:56Z http://agritrop.cirad.fr/578221/ http://agritrop.cirad.fr/578221/ Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidisin wild Asian amphibians. Bataille Arnaud, Fong Jonathan J., Cha Moonsuk, Wogan Guinevere O.U., Baek Hae Jun, Lee Hang, Min Mi-Sook, Waldman Bruce. 2013. Molecular Ecology, 22 (16) : 4196-4209.https://doi.org/10.1111/mec.12385 <https://doi.org/10.1111/mec.12385> Researchers Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidisin wild Asian amphibians Bataille, Arnaud Fong, Jonathan J. Cha, Moonsuk Wogan, Guinevere O.U. Baek, Hae Jun Lee, Hang Min, Mi-Sook Waldman, Bruce eng 2013 Molecular Ecology L73 - Maladies des animaux L20 - Écologie animale Population declines and extinctions of amphibians have been attributed to the chytrid fungus Batrachochytrium dendrobatidis (Bd), especially one globally emerging recombinant lineage ('Bd-GPL'). We used PCR assays that target the ribosomal internal transcribed spacer region (ITS) of Bd to determine the prevalence and genetic diversity of Bd in South Korea, where Bd is widely distributed but is not known to cause morbidity or mortality in wild populations. We isolated Korean Bd strains from native amphibians with low infection loads and compared them to known worldwide Bd strains using 19 polymorphic SNP and microsatellite loci. Bd prevalence ranged between 12.5 and 48.0%, in 11 of 17 native Korean species, and 24.7% in the introduced bullfrog Lithobates catesbeianus. Based on ITS sequence variation, 47 of the 50 identified Korean haplotypes formed a group closely associated with a native Brazilian Bd lineage, separated from the Bd-GPL lineage. However, multilocus genotyping of three Korean Bd isolates revealed strong divergence from both Bd-GPL and the native Brazilian Bd lineages. Thus, the ITS region resolves genotypes that diverge from Bd-GPL but otherwise generates ambiguous phylogenies. Our results point to the presence of highly diversified endemic strains of Bd across Asian amphibian species. The rarity of Bd-GPL-associated haplotypes suggests that either this lineage was introduced into Korea only recently or Bd-GPL has been outcompeted by native Bd strains. Our results highlight the need to consider possible complex interactions among native Bd lineages, Bd-GPL and their associated amphibian hosts when assessing the spread and impact of Bd-GPL on worldwide amphibian populations. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/578221/7/578221_version_editee.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/mec.12385 10.1111/mec.12385 info:eu-repo/semantics/altIdentifier/doi/10.1111/mec.12385 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/mec.12385 info:eu-repo/semantics/dataset/purl/http://www.ncbi.nlm.nih.gov/nuccore/jX983045 info:eu-repo/semantics/dataset/purl/http://www.ncbi.nlm.nih.gov/nuccore/JX983089 |
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L73 - Maladies des animaux L20 - Écologie animale L73 - Maladies des animaux L20 - Écologie animale Bataille, Arnaud Fong, Jonathan J. Cha, Moonsuk Wogan, Guinevere O.U. Baek, Hae Jun Lee, Hang Min, Mi-Sook Waldman, Bruce Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidisin wild Asian amphibians |
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Population declines and extinctions of amphibians have been attributed to the chytrid fungus Batrachochytrium dendrobatidis (Bd), especially one globally emerging recombinant lineage ('Bd-GPL'). We used PCR assays that target the ribosomal internal transcribed spacer region (ITS) of Bd to determine the prevalence and genetic diversity of Bd in South Korea, where Bd is widely distributed but is not known to cause morbidity or mortality in wild populations. We isolated Korean Bd strains from native amphibians with low infection loads and compared them to known worldwide Bd strains using 19 polymorphic SNP and microsatellite loci. Bd prevalence ranged between 12.5 and 48.0%, in 11 of 17 native Korean species, and 24.7% in the introduced bullfrog Lithobates catesbeianus. Based on ITS sequence variation, 47 of the 50 identified Korean haplotypes formed a group closely associated with a native Brazilian Bd lineage, separated from the Bd-GPL lineage. However, multilocus genotyping of three Korean Bd isolates revealed strong divergence from both Bd-GPL and the native Brazilian Bd lineages. Thus, the ITS region resolves genotypes that diverge from Bd-GPL but otherwise generates ambiguous phylogenies. Our results point to the presence of highly diversified endemic strains of Bd across Asian amphibian species. The rarity of Bd-GPL-associated haplotypes suggests that either this lineage was introduced into Korea only recently or Bd-GPL has been outcompeted by native Bd strains. Our results highlight the need to consider possible complex interactions among native Bd lineages, Bd-GPL and their associated amphibian hosts when assessing the spread and impact of Bd-GPL on worldwide amphibian populations. |
format |
article |
topic_facet |
L73 - Maladies des animaux L20 - Écologie animale |
author |
Bataille, Arnaud Fong, Jonathan J. Cha, Moonsuk Wogan, Guinevere O.U. Baek, Hae Jun Lee, Hang Min, Mi-Sook Waldman, Bruce |
author_facet |
Bataille, Arnaud Fong, Jonathan J. Cha, Moonsuk Wogan, Guinevere O.U. Baek, Hae Jun Lee, Hang Min, Mi-Sook Waldman, Bruce |
author_sort |
Bataille, Arnaud |
title |
Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidisin wild Asian amphibians |
title_short |
Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidisin wild Asian amphibians |
title_full |
Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidisin wild Asian amphibians |
title_fullStr |
Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidisin wild Asian amphibians |
title_full_unstemmed |
Genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus Batrachochytrium dendrobatidisin wild Asian amphibians |
title_sort |
genetic evidence for a high diversity and wide distribution of endemic strains of the pathogenic chytrid fungus batrachochytrium dendrobatidisin wild asian amphibians |
url |
http://agritrop.cirad.fr/578221/ http://agritrop.cirad.fr/578221/7/578221_version_editee.pdf |
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