Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae
The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound.
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Subjects: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, Oryza sativa, Magnaporthe, expression des gènes, avirulence, interactions biologiques, relation hôte pathogène, résistance aux maladies, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_31827, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_35004, http://aims.fao.org/aos/agrovoc/c_49896, http://aims.fao.org/aos/agrovoc/c_34017, http://aims.fao.org/aos/agrovoc/c_2328, |
Online Access: | http://agritrop.cirad.fr/577195/ http://agritrop.cirad.fr/577195/1/Song%20Heterologous%20exp%20Ace1%20Chemical%20Sc%202015.pdf |
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dig-cirad-fr-5771952024-01-28T22:50:16Z http://agritrop.cirad.fr/577195/ http://agritrop.cirad.fr/577195/ Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae. Song Zhongshu, Bakeer Walid, Marshall James W., Yakasai Ahmed A., Mohd Khalid Rozida, Collemare Jérôme, Skellam Elizabeth, Tharreau Didier, Lebrun Marc-Henri, Lazarus Colin M., Bailey Andrew M., Simpson Thomas J., Cox Russel J.. 2015. Chemical Science, 6 (8) : 4837-4845.https://doi.org/10.1039/c4sc03707c <https://doi.org/10.1039/c4sc03707c> Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae Song, Zhongshu Bakeer, Walid Marshall, James W. Yakasai, Ahmed A. Mohd Khalid, Rozida Collemare, Jérôme Skellam, Elizabeth Tharreau, Didier Lebrun, Marc-Henri Lazarus, Colin M. Bailey, Andrew M. Simpson, Thomas J. Cox, Russel J. eng 2015 Chemical Science H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Oryza sativa Magnaporthe expression des gènes avirulence interactions biologiques relation hôte pathogène résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35004 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2328 The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/577195/1/Song%20Heterologous%20exp%20Ace1%20Chemical%20Sc%202015.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1039/c4sc03707c 10.1039/c4sc03707c info:eu-repo/semantics/altIdentifier/doi/10.1039/c4sc03707c info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1039/c4sc03707c |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Oryza sativa Magnaporthe expression des gènes avirulence interactions biologiques relation hôte pathogène résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35004 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2328 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Oryza sativa Magnaporthe expression des gènes avirulence interactions biologiques relation hôte pathogène résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35004 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2328 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Oryza sativa Magnaporthe expression des gènes avirulence interactions biologiques relation hôte pathogène résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35004 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2328 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Oryza sativa Magnaporthe expression des gènes avirulence interactions biologiques relation hôte pathogène résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35004 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2328 Song, Zhongshu Bakeer, Walid Marshall, James W. Yakasai, Ahmed A. Mohd Khalid, Rozida Collemare, Jérôme Skellam, Elizabeth Tharreau, Didier Lebrun, Marc-Henri Lazarus, Colin M. Bailey, Andrew M. Simpson, Thomas J. Cox, Russel J. Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae |
description |
The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound. |
format |
article |
topic_facet |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Oryza sativa Magnaporthe expression des gènes avirulence interactions biologiques relation hôte pathogène résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_31827 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35004 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2328 |
author |
Song, Zhongshu Bakeer, Walid Marshall, James W. Yakasai, Ahmed A. Mohd Khalid, Rozida Collemare, Jérôme Skellam, Elizabeth Tharreau, Didier Lebrun, Marc-Henri Lazarus, Colin M. Bailey, Andrew M. Simpson, Thomas J. Cox, Russel J. |
author_facet |
Song, Zhongshu Bakeer, Walid Marshall, James W. Yakasai, Ahmed A. Mohd Khalid, Rozida Collemare, Jérôme Skellam, Elizabeth Tharreau, Didier Lebrun, Marc-Henri Lazarus, Colin M. Bailey, Andrew M. Simpson, Thomas J. Cox, Russel J. |
author_sort |
Song, Zhongshu |
title |
Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae |
title_short |
Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae |
title_full |
Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae |
title_fullStr |
Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae |
title_full_unstemmed |
Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae |
title_sort |
heterologous expression of the avirulence gene ace1 from the fungal rice pathogen magnaporthe oryzae |
url |
http://agritrop.cirad.fr/577195/ http://agritrop.cirad.fr/577195/1/Song%20Heterologous%20exp%20Ace1%20Chemical%20Sc%202015.pdf |
work_keys_str_mv |
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