Development of elite indica rice lines with wide spectrum of resistance to Thai blast isolates by pyramiding multiple resistance QTLs

Development of elite indica rice lines with wide spectrum of resistance to Thai blast isolates by pyramiding multiple resistance QTLs

The rice varieties IR64 and Jao Hom Nin (JHN) demonstrated a broad-spectrum resistance against the rice blast pathogens in Thailand. A genomic investigation unravelled many resistance genes residing on four genomic regions, chromosome 2 and 12 in IR64 and 1 and 11 in JHN. A cross between these varieties was made to combine resistance genes into a single genotype. Marker-assisted selection (MAS) was employed to identify F2 and F3 plants carrying a combination of four resistance QTLs in a homozygous fusion. Flanking markers RM212/RM319 and RM144/RM139 to blast resistant QTLs on chromosome 1 and 11 in JHN rice and tightly-linked markers RM208 and RM179 to blast resistant QTLs on chromosome 2 and 12 in IR64 rice variety were used for MAS. The stepwise MAS screening was brought in as a strategy to provide a cost-saving and minimum number of PCR performing to select resistant genotypes. F4 generation, lines carrying all resistant QTLs show a broader spectrum of resistance against 11 representatives of Thai blast pathogen isolates.

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Bibliographic Details
Main Authors: Sreewongchai, Tanee, Toojinda, Theerayut, Thanintorn, N., Kosawang, C., Vanavichit, Apichart, Tharreau, Didier, Sirithunya, Pattama
Format: article biblioteca
Language:eng
Subjects:F30 - Génétique et amélioration des plantes, H20 - Maladies des plantes, Oryza sativa, résistance aux maladies, résistance génétique, amélioration des plantes, méthode d'amélioration génétique, sélection, marqueur génétique, variété, croisement, locus des caractères quantitatifs, Magnaporthe grisea, phénotype, génotype, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_35130, http://aims.fao.org/aos/agrovoc/c_5956, http://aims.fao.org/aos/agrovoc/c_1079, http://aims.fao.org/aos/agrovoc/c_6951, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_8157, http://aims.fao.org/aos/agrovoc/c_1976, http://aims.fao.org/aos/agrovoc/c_37974, http://aims.fao.org/aos/agrovoc/c_37090, http://aims.fao.org/aos/agrovoc/c_5776, http://aims.fao.org/aos/agrovoc/c_3225, http://aims.fao.org/aos/agrovoc/c_7701,
Online Access:http://agritrop.cirad.fr/554472/
http://agritrop.cirad.fr/554472/1/document_554472.pdf
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Summary:The rice varieties IR64 and Jao Hom Nin (JHN) demonstrated a broad-spectrum resistance against the rice blast pathogens in Thailand. A genomic investigation unravelled many resistance genes residing on four genomic regions, chromosome 2 and 12 in IR64 and 1 and 11 in JHN. A cross between these varieties was made to combine resistance genes into a single genotype. Marker-assisted selection (MAS) was employed to identify F2 and F3 plants carrying a combination of four resistance QTLs in a homozygous fusion. Flanking markers RM212/RM319 and RM144/RM139 to blast resistant QTLs on chromosome 1 and 11 in JHN rice and tightly-linked markers RM208 and RM179 to blast resistant QTLs on chromosome 2 and 12 in IR64 rice variety were used for MAS. The stepwise MAS screening was brought in as a strategy to provide a cost-saving and minimum number of PCR performing to select resistant genotypes. F4 generation, lines carrying all resistant QTLs show a broader spectrum of resistance against 11 representatives of Thai blast pathogen isolates.

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